دورية أكاديمية
CXCL12 secreted by pancreatic stellate cells accelerates gemcitabine resistance of pancreatic cancer by enhancing glycolytic reprogramming
| العنوان: | CXCL12 secreted by pancreatic stellate cells accelerates gemcitabine resistance of pancreatic cancer by enhancing glycolytic reprogramming |
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| المؤلفون: | Xiangyu Lu, Yilei Wu, Rui Cao, Xiaojiong Yu, Jun Gong |
| المصدر: | Animal Cells and Systems, Vol 26, Iss 4, Pp 148-157 (2022) |
| بيانات النشر: | Taylor & Francis Group, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Medicine (General) LCC:Biology (General) |
| مصطلحات موضوعية: | Pancreatic stellate cells, CXCL12, glycolysis, resistance, pancreatic cancer, Medicine (General), R5-920, Biology (General), QH301-705.5 |
| الوصف: | Pancreatic stellate cells (PSCs) are the primary cell components of pancreatic cancer (PC) and are involved in tumor growth, metastasis and resistance. However, the role and the mechanism of PSCs in gemcitabine (GEM) resistance to PC still need more investigation. We found that CXCL12 mRNA and secreted CXCL12 protein were higher in PSCs after GEM treatment. The conditioned medium (CM) from GEM-treated PSCs reduced the GEM sensitivity of PC cells. Blocking of CXCL12 in CM by anti-CXCL12 antibody partly restored the GEM sensitivity of PC cells. Blocking of CXCL12 decreased glucose consumption, lactate production, ECAR, and glycolysis-related gene expression in PC cells. The PI3K/AKT/mTOR pathway was activated by the binding of CXCL12 and CXCR4. Moreover, CXCR4 mRNA and protein expressions in PC cells were increased after GEM treatment. Our results indicated the cross-talk between PSCs and PC cells during GEM chemotherapy. CXCL12 secreted by PSCs reduces GEM sensitivity of PC cells by binding to CXCR4 and activating PI3K/AKT/mTOR-glycolysis pathway in PC. Our findings would lay the foundation for solving GEM resistance in PC. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 19768354 2151-2485 1976-8354 |
| العلاقة: | https://doaj.org/toc/1976-8354Test; https://doaj.org/toc/2151-2485Test |
| DOI: | 10.1080/19768354.2022.2091019 |
| الوصول الحر: | https://doaj.org/article/c225b42dd0e04f26a8d37fdddedb5d0aTest |
| رقم الانضمام: | edsdoj.225b42dd0e04f26a8d37fdddedb5d0a |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 19768354 21512485 |
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| DOI: | 10.1080/19768354.2022.2091019 |