دورية أكاديمية
菊芋SRAP-PCR反应体系优化及引物筛选
| العنوان: | 菊芋SRAP-PCR反应体系优化及引物筛选 |
|---|---|
| المؤلفون: | 韩睿, 赵孟良, 马胜超, 李莉 |
| المصدر: | 《分子植物育种》网络版; MPB 2012 VOL10 |
| بيانات النشر: | Sophia Publishing Group Inc. |
| سنة النشر: | 2012 |
| المجموعة: | Biopublisher / 中文出版平台在线稿件处理系统 |
| مصطلحات موضوعية: | 菊芋；SRAP-PCR；反应体系；优化；引物筛选 |
| الوصف: | 本研究以菊芋为材料，通过单因子优化试验和L16(45)正交试验设计对影响SRAP-PCR扩增结果的重要参数进行探讨，建立了适合菊芋SRAP-PCR分析的反应体系，即20 μL反应体系中含10×PCR 扩增缓冲液，2.5 mmol/L Mg2+，0.24 mmol/L dNTPs，0.25 μmol/L正反引物，0.8 U TaqDNA聚合酶和80 ng模板DNA。利用该优化体系进行稳定性检测，并从110 个SRAP 引物组合中筛选出100个具有扩增条带清晰丰富且重复性好的引物组合和22个清晰且多态性高的引物组合，表明该体系稳定可靠，并且能够有效地用于菊芋种质资源鉴定及遗传多样性分析等研究。 |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | Chinese |
| العلاقة: | https://biopublisher.cn/index.php/mpb/article/view/735/pdf_84Test; https://biopublisher.cn/index.php/mpb/article/downloadSuppFile/735/166Test; https://biopublisher.cn/index.php/mpb/article/downloadSuppFile/735/167Test; https://biopublisher.cn/index.php/mpb/article/view/735Test |
| الإتاحة: | https://biopublisher.cn/index.php/mpb/article/view/735Test |
| حقوق: | Authors retain copyright for any article that is published with open access.Authors grant Sophia a license to publish the article and identify itself as the original publisher.Authors also grant any third party the right to use the article freely as long as its integrity is maintained and its original authors, citation details and publisher are identified.The Sophia Copyright and License Agreement (identical to the Creative Commons Attribution License) formalizes these and other terms and conditions. |
| رقم الانضمام: | edsbas.579E7476 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |