دورية أكاديمية
Development and validation of a capillary electrophoresis method for the characterization of herpes simplex virus type 1 (HSV-1) thymidine kinase substrates and inhibitors
| العنوان: | Development and validation of a capillary electrophoresis method for the characterization of herpes simplex virus type 1 (HSV-1) thymidine kinase substrates and inhibitors |
|---|---|
| المؤلفون: | Iqbal, Jamshed, Scapozza, Leonardo, Folkers, Gerd, Müller, Christa E. |
| المصدر: | ISSN: 1570-0232 ; Journal of chromatography. B, vol. 846, no. 1-2 (2007) p. 281-290. |
| سنة النشر: | 2007 |
| المجموعة: | Université de Genève: Archive ouverte UNIGE |
| مصطلحات موضوعية: | info:eu-repo/classification/ddc/615, Electrophoresis, Capillary/methods, Enzyme Inhibitors/analysis, Herpesvirus 1, Human/enzymology, Kinetics, Reference Standards, Reproducibility of Results, Sensitivity and Specificity, Substrate Specificity, Thymidine Kinase/antagonists & inhibitors/metabolism |
| الوصف: | A fast, convenient capillary electrophoresis (CE) method was developed for monitoring the enzymatic reaction of herpes simplex virus type 1 thymidine kinase (HSV-1 TK). The reaction was performed in a test tube followed by quantitative analysis of the products. The optimized CE conditions were as follows: polyacrylamide-coated capillary (20 cm effective length x 50 microm), electrokinetic injection for 30s, 50 mM phosphate buffer at pH 6.5, constant current of -60 microA, UV detection at 210 nm, UMP or cAMP were used as internal standards. Phosphorylated products eluted within less than 7 min. The limits of detection were 0.36 microM for dTMP and 0.86 microM for GMP. The method was used to study enzyme kinetics, and to investigate alternative substrates and inhibitors. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/17023224; https://archive-ouverte.unige.ch/unige:14364Test; unige:14364 |
| الإتاحة: | https://doi.org/10.1016/j.jchromb.2006.09.018Test https://archive-ouverte.unige.ch/unige:14364Test |
| حقوق: | info:eu-repo/semantics/restrictedAccess |
| رقم الانضمام: | edsbas.499E2976 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |