Galectin-3, a marker for vacuole lysis by invasive pathogens
| العنوان: | Galectin-3, a marker for vacuole lysis by invasive pathogens |
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| المؤلفون: | Marie-Christine Prévost, Irit Paz, Philippe J. Sansonetti, Jost Enninga, Joëlle Mounier, Cecilia Cederfur, Martin Sachse, Hakon Leffler, Françoise Poirier, Frank Lafont, Nicolas Dupont |
| المساهمون: | Institut Pasteur [Paris], Institut Jacques Monod (IJM (UMR_7592)), Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Chaire Microbiologie et Maladies infectieuses, Collège de France (CdF (institution)), Institut Pasteur [Paris] (IP), Collège de France - Chaire Microbiologie et Maladies infectieuses |
| المصدر: | Cellular Microbiology Cellular Microbiology, Wiley, 2010, 12 (4), pp.530-44. ⟨10.1111/j.1462-5822.2009.01415.x⟩ Cellular Microbiology, 2010, 12 (4), pp.530-44. ⟨10.1111/j.1462-5822.2009.01415.x⟩ |
| بيانات النشر: | HAL CCSD, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Lysis, Listeria, media_common.quotation_subject, Galectin 3, Immunology, Vacuole, Biology, Microbiology, Cell Line, 03 medical and health sciences, Mice, Cricetulus, Dogs, Polysaccharides, Salmonella, Virology, Cricetinae, Phagosomes, otorhinolaryngologic diseases, Animals, Humans, Secretion, Internalization, Microscopy, Immunoelectron, 030304 developmental biology, Galectin, Phagosome, media_common, 0303 health sciences, 030302 biochemistry & molecular biology, Amino Sugars, Intracellular Membranes, Cell biology, Cytosol, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Cytoplasm, Shigella, Biomarkers, Protein Binding |
| الوصف: | International audience; Shigella bacteria invade macrophages and epithelial cells and following internalization lyse the phagosome and escape to the cytoplasm. Galectin-3, an abundant protein in macrophages and epithelial cells, belongs to a family of beta-galactoside-binding proteins, the galectins, with many proposed functions in immune response, development, differentiation, cancer and infection. Galectins are synthesized as cytosolic proteins and following non-classical secretion bind extracellular beta-galactosides. Here we analysed the localization of galectin-3 following entry of Shigella into the cytosol and detected a striking phenomenon. Very shortly after bacterial invasion, intracellular galectin-3 accumulated in structures in vicinity to internalized bacteria. By using immuno-electron microscopy analysis we identified galectin-3 in membranes localized in the phagosome and in tubules and vesicles that derive from the endocytic pathway. We also demonstrated that the binding of galectin-3 to host N-acetyllactosamine-containing glycans, was required for forming the structures. Accumulation of the structures was a type three secretion system-dependent process. More specifically, existence of structures was strictly dependent upon lysis of the phagocytic vacuole and could be shown also by Gram-positive Listeria and Salmonella sifA mutant. We suggest that galectin-3-containing structures may serve as a potential novel tool to spot vacuole lysis. |
| اللغة: | English |
| تدمد: | 1462-5814 1462-5822 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e834f17537372993637619ff7293472aTest https://hal.archives-ouvertes.fr/hal-00486248Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....e834f17537372993637619ff7293472a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14625814 14625822 |
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