Detection of erbB2 copy number variations in plasma of patients with esophageal carcinoma
| العنوان: | Detection of erbB2 copy number variations in plasma of patients with esophageal carcinoma |
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| المؤلفون: | Massimo Zollo, Loredana Vecchione, Giancarlo Troncone, Pasqualino De Antonellis, Donatella Montanaro, Immacolata Andolfo, Michele Orditura, Marica Gemei, Achille Iolascon, Fortunato Ciardiello, Giuseppe Petrosino, Mario Capasso, Fernando De Vita |
| المساهمون: | Andolfo, I, Petrosino, Giuseppe, Vecchione, L, De Antonellis, P, Capasso, Mario, Montanaro, D, Gemei, M, Troncone, Giancarlo, Iolascon, Achille, Orditura, M, Ciardiello, F, De Vita, F, Zollo, Massimo, Petrosino, G, DE ANTONELLIS, P, Capasso, M, Troncone, G, Iolascon, A, Orditura, Michele, Ciardiello, Fortunato, DE VITA, Ferdinando, Zollo, M. |
| المصدر: | BMC Cancer BMC Cancer, Vol 11, Iss 1, p 126 (2011) Europe PubMed Central |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Male, Vascular Endothelial Growth Factor A, Oncology, Cancer Research, Esophageal Neoplasms, erbB2 copy number variation, Gene Dosage, 0302 clinical medicine, Surgical oncology, Copy-number variation, Early Detection of Cancer, 0303 health sciences, Reverse Transcriptase Polymerase Chain Reaction, Middle Aged, Esophageal cancer, Prognosis, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, 3. Good health, Cell-free fetal DNA, 030220 oncology & carcinogenesis, Female, CTCs, Research Article, medicine.medical_specialty, Sensitivity and Specificity, Gene dosage, lcsh:RC254-282, Disease-Free Survival, cell-free DNA, 03 medical and health sciences, esophageal carcinoma, Internal medicine, Biomarkers, Tumor, Genetics, medicine, Carcinoma, Humans, Progression-free survival, Aged, 030304 developmental biology, business.industry, DNA, Genes, erbB-2, medicine.disease, Tumor progression, business, prognostic marker |
| الوصف: | Background Mortality is high in patients with esophageal carcinoma as tumors are rarely detected before the disease has progressed to an advanced stage. Here, we sought to isolate cell-free DNA released into the plasma of patients with esophageal carcinoma, to analyze copy number variations of marker genes in the search for early detection of tumor progression. Methods Plasma of 41 patients with esophageal carcinoma was prospectively collected before tumor resection and chemotherapy. Our dataset resulted heterogeneous for clinical data, resembling the characteristics of the tumor. DNA from the plasma was extracted to analyze copy number variations of the erbB2 gene using real-time PCR assays. Results The real-time PCR assays for erbB2 gene showed significant (P = 0.001) copy number variations in the plasma of patients with esophageal carcinoma, as compared to healthy controls with high sensitivity (80%) and specificity (95%). These variations in erbB2 were negatively correlated to the progression free survival of these patients (P = 0.03), and revealed a further risk category stratification of patients with low VEGF expression levels. Conclusion The copy number variation of erbB2 gene from plasma can be used as prognostic marker for early detection of patients at risk of worse clinical outcome in esophageal cancer. |
| اللغة: | English |
| تدمد: | 1471-2407 |
| DOI: | 10.1186/1471-2407-11-126 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a2d5c913c824e8f93f8156264377b14fTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....a2d5c913c824e8f93f8156264377b14f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14712407 |
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| DOI: | 10.1186/1471-2407-11-126 |