دورية أكاديمية
Autoantibodies against the autophagic protein microtubule-associated light-chain 3 (LC3): Immunocharacterization of an atypical ANA pattern
| العنوان: | Autoantibodies against the autophagic protein microtubule-associated light-chain 3 (LC3): Immunocharacterization of an atypical ANA pattern |
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| المؤلفون: | Emilia Maellaro, Lucia Terzuoli, Maria Romana Bacarelli, Barbara Del Bello, Nicola Bizzaro, Brunetta Porcelli |
| المصدر: | Autoimmunity, Vol 53, Iss 5, Pp 245-252 (2020) |
| بيانات النشر: | Taylor & Francis Group, 2020. |
| سنة النشر: | 2020 |
| المجموعة: | LCC:Internal medicine |
| مصطلحات موضوعية: | autoantibodies, antinuclear antibodies (ana), ana patterns, microtubule-associated light-chain 3 protein (lc3), autophagy, Internal medicine, RC31-1245 |
| الوصف: | Autoantibodies to nuclear and cytoplasmic antigens are commonly detected by indirect immunofluorescence (IIF) on HEp-2 cells, and three major staining patterns (nuclear, cytoplasmic, and mitotic) are distinguished. Here, we report an atypical cytoplasmic pattern, not described so far, observed in the serum of a patient with a controversial diagnosis of systemic lupus erythematosus (SLE). Moreover, for the first time, we have revealed the presence of autoantibodies against the microtubule-associated light-chain 3 (LC3) protein, which plays a key role in the autophagic process. The target antigen has been identified in IIF by means of a competition test using purified anti-LC3 antibodies on HEp-2 cells, and confirmed by Western blot analysis using cellular or recombinant LC3 as antigen, immunoreacted with the patient’s serum. The identification of this atypical pattern and the related autoantibody-antigen system sheds new light on autophagy, which is increasingly considered to be involved in the etiopathogenesis of autoimmune disorders, and could contribute to select more personalized therapies. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 0891-6934 1607-842X 08916934 93881460 |
| العلاقة: | https://doaj.org/toc/0891-6934Test; https://doaj.org/toc/1607-842XTest |
| DOI: | 10.1080/08916934.2020.1755963 |
| الوصول الحر: | https://doaj.org/article/7e3ff6b93881460191b09610447b6ee1Test |
| رقم الانضمام: | edsdoj.7e3ff6b93881460191b09610447b6ee1 |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 08916934 1607842X 93881460 |
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| DOI: | 10.1080/08916934.2020.1755963 |