المؤلفون: Shi, Wenjia, Ma, Yao, Hu, Peilin, Pang, Mi, Huang, Xiaona, Dang, Yiting, Xie, Yuxin, Wu, Danni

مصطلحات موضوعية: Quantitative Biology - Molecular Networks

الوصف: Hill function is one of the widely used gene transcription regulation models. Its attribute of fitting may result in a lack of an underlying physical picture, yet the fitting parameters can provide information about biochemical reactions, such as the number of transcription factors (TFs) and the binding energy between regulatory elements. However, it remains unclear when and how much biochemical information can Hill function provide in addition to fitting. Here, started from the interactions between TFs and RNA polymerase during transcription regulation and both of their association-dissociation reactions at specific/nonspecific sites on DNA, the regulatory effect of TFs was deduced as fold change. We found that, for weak promoter, fold change can degrade into the regulatory factor (Freg) which is closely correlated with Hill function. By directly comparing and fitting with Hill function, the fitting parameters and corresponding biochemical reaction parameters in Freg were analyzed and discussed, where the single TF and multiple TFs that with cooperativity and basic logic effects were considered. We concluded the strength of promoter and interactions between TFs determine whether Hill function can reflect the corresponding biochemical information. Our findings highlight the role of Hill function in modeling/fitting for transcriptional regulation, which also benefits the preparation of synthetic regulatory elements.

الوصول الحر: http://arxiv.org/abs/2403.01702Test

المؤلفون: Liu, Xiaoxi, Huang, Xiaona, Yang, Yuning, Narayan, Anuradha, Du‐Skabrin, Lidan, Ding, Xue, Chen, Yongchao, Zhao, Jun, Chang, Suying, Wang, Fang

المصدر: Maternal & Child Nutrition ; ISSN 1740-8695 1740-8709

الوصف: Maternal anaemia is a major public health problem. Developing maternal anaemia prevention and control policies is an important prerequisite for carrying out evidence‐based interventions. This article reviews maternal anaemia prevention and control policies in China, identifies gaps, and provides references for other countries. We examined policies concerning maternal nutrition and other related literature in China, identified through key databases and government websites, and conducted a narrative review of the relevant documentations guided by the Smith Policy‐Implementing‐Process framework. A total of 65 articles and documents were identified for analysis. We found that Chinese government has committed to reducing maternal anaemia at the policy level, with established objectives and a clear time frame. However, most of policies were not accompanied by operational guidelines, standardized interventions, and vigorous monitoring and evaluation mechanisms, and 85% of the policies don't have quantifiable objectives on anaemia. Maternal anaemia prevention and control services offered in clinical settings were primarily nutrition education and anaemia screening. Population‐based interventions such as iron fortification have yet to be scaled up. Furthermore, medical insurance schemes in some regions do not cover anaemia prevention and treatment, and in other regions that offer coverage, the reimbursement rate is low. The number and capacity of health professionals is also limited. Policy changes should focus on the integration of evidence‐based interventions into routine antenatal care services and public health service packages, standardization of dosages and provision of iron supplementation, streamline of reimbursement for outpatient expenses, and capacity building of health professionals.

الإتاحة: https://doi.org/10.1111/mcn.13653Test

المؤلفون: Wang, Yibo, Huang, Xiaona, Liu, Yan, Zhang, Xiangyu, Yang, Bing, Yue, Yanan

المساهمون: Natural Science Foundation of Hubei Province, Wuhan University, Fundamental Research Funds for the Central Universities, National Natural Science Foundation of China

المصدر: Energy Storage and Saving ; volume 3, issue 1, page 52-59 ; ISSN 2772-6835

الإتاحة: https://doi.org/10.1016/j.enss.2023.06.002Test
https://api.elsevier.com/content/article/PII:S2772683523000316?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S2772683523000316?httpAccept=text/plainTest

المؤلفون: Liu, Ruqin, Wu, Yanqing, Wang, Xinjie, Huang, Fenglei, Huang, Xiaona, Wen, Yushi

المساهمون: Natural Science Foundation of Hubei Province, National Natural Science Foundation of China

المصدر: Defence Technology ; volume 33, page 278-294 ; ISSN 2214-9147

مصطلحات موضوعية: Metals and Alloys, Mechanical Engineering, Ceramics and Composites, Computational Mechanics

الإتاحة: https://doi.org/10.1016/j.dt.2023.02.011Test
https://api.elsevier.com/content/article/PII:S2214914723000405?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S2214914723000405?httpAccept=text/plainTest

المؤلفون: Zhou, Hong, Qu, Xueqi, Yang, Yuning, KC, Ashish, 1982, Liu, Xiaoli, Yang, Chenlu, Wang, Anqi, Huang, Yue, Sun, Jing, Huang, Xiaona, Wang, Yan

المصدر: Nutritional neuroscience. 25(1):70-79

مصطلحات موضوعية: China, Developmental delay, Diet types, Moderate to late preterm

الوصف: Aim: To measure the development of moderate to late preterm children by Ages and Stages Questionnaires (ASQ) and explore the relationship between moderate to late preterm, diet types and development delay in less-developed rural China.Methods: Data were collected from a cross-sectional community-based survey, which recruited 1748 children aged 1-59 months in eight counties of China. Caregivers of these children completed the Chinese version of ASQ-3 (ASQ-C) while physical examination and questionnaires on socio-demographic characteristics were conducted. Multivariate logistic regressions were used to analyze the association between moderate to late preterm and suspected developmental delay, as well as the association between diet types and suspected developmental delay. Consumption of certain food types was compared between moderate to late preterm and full-term children.Results: The prevalence of suspected overall developmental delay was 31.3% in the moderate to the late preterm group, compared with 21.6% in the full-term group. Moderate to late preterm birth was not associated with total suspected developmental delay and developmental delay in all the domains of ASQ, except for fine motor (OR = 2.43 95% C.I.: 1.04-5.56). The intake of vegetables and fruits had a protective influence on developmental delay in fine motor function, and moderate to late preterm children had lower relative consumption of fruits and vegetables than full-term children.Conclusion: Moderate to late preterm children in rural China showed an increased likelihood of developmental delay in fine motor function. Future interventions to improve the intake of vegetables and fruits in moderate to late preterm children are recommended.

وصف الملف: electronic

الوصول الحر: https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-416124Test
https://doi.org/10.1080/1028415X.2020.1712534Test
https://uu.diva-portal.org/smash/get/diva2:1453528/FULLTEXT01.pdfTest

المؤلفون: Gao, Hui, Zeng, Yuxiao, Huang, Xiaona, A, Luodan, Liang, Qingle, Xie, Jing, Lin, Xi, Gong, Jing, Fan, Xiaotang, Zou, Ting, Xu, Haiwei

المساهمون: National Natural Science Foundation of China, National Key Research and Development Program of China

المصدر: Journal of Extracellular Vesicles ; volume 13, issue 1 ; ISSN 2001-3078 2001-3078

الوصف: Retinal degeneration (RD), a group of diseases leading to irreversible vision loss, is characterised by retinal pigment epithelium (RPE) or retinal neuron damage and loss. With fewer risks of immune rejection and tumorigenesis, stem cell‐secreted extracellular vesicles (EVs) offer a new cell‐free therapeutic paradigm for RD, which remains to be investigated. Human retinal organoid‐derived retinal progenitor cells (hERO‐RPCs) are an easily accessible and advanced cell source for RD treatment. However, hERO‐RPCs‐derived EVs require further characterisation. Here, we compared the characteristics of EVs from hERO‐RPCs (hRPC‐EVs) with those of human embryonic stem cell (hESC)‐derived EVs (hESC‐EVs) as controls. Based on in‐depth proteomic analysis, we revealed remarkable differences between hRPC‐EVs and hESC‐EVs. A comparison between EVs and their respective cells of origin demonstrated that the protein loading of hRPC‐EVs was more selective than that of hESC‐EVs. In particular, hESC‐EVs were enriched with proteins related to angiogenesis and cell cycle, whereas hRPC‐EVs were enriched with proteins associated with immune modulation and retinal development. More importantly, compared with that of hESC‐EVs, hRPC‐EVs exhibited a lower correlation with cell proliferation and a unique capacity to regulate lipid metabolism. It was further confirmed that hRPC‐EVs potentially eliminated lipid deposits, inhibited lipotoxicity and oxidative stress, and enhanced phagocytosis and survival of oleic acid‐treated ARPE‐19 cells. Mechanistically, hRPC‐EVs are integrated into the mitochondrial network of oleic acid‐treated ARPE‐19 cells, and increased the level of mitochondrial fatty acid β‐oxidation‐related proteins. Thus, organoid‐derived hRPC‐EVs represent a promising source of cell‐free therapy for RD, especially for blinding diseases related to abnormal lipid metabolism in RPE cells.

الإتاحة: https://doi.org/10.1002/jev2.12401Test

المؤلفون: Wang, Wen, Wang, Yinghang, Zhang, Hanxiyue, Yang, Ge, Lin, Yun, Wang, Chenran, Huang, Xiaona, Tian, Xiaobo, Xiao, Angela Y., Xu, Tao, Tang, Kun

المساهمون: Safe Neonatal Project of United Nations Children’s Fund Office for China

المصدر: BMC Pregnancy and Childbirth ; volume 23, issue 1 ; ISSN 1471-2393

مصطلحات موضوعية: Obstetrics and Gynecology

الوصف: Background Implementation of Kangaroo Mother Care (KMC) in resource-limited areas of China may face unique barriers, such as a lack of resources, geographic location and more traditional culture among others. This qualitative study analyses the facilitators and barriers to implementing KMC in county-level health facilities in resource-limited areas of China for the promotion of KMC on a larger scale. Methods Participants from 4 of the 18 pilot counties where early essential newborn care was implemented through the Safe Neonatal Project and 4 control counties not enrolled in Safe Neonatal Project were selected using purposive sampling. A total of 155 participants were interviewed, including stakeholders of the Safe Neonatal Project such as national maternal health experts, relevant government officials and medical staff. Thematic analysis was used to process and analyse the interview content in order to summarise the facilitators and barriers to implementing KMC. Results KMC was accepted in the pilot areas but still faced certain challenges due to institutional regulation, resource provision and the perceptions of health staff, postpartum mothers and their families as well as COVID-19 prevention and control regulations. The facilitators identified were government officials and medical staff acceptance and the incorporation of KMC into routine clinical care. The barriers identified were a lack of dedicated funding and other resources, the present scope of health insurance and KMC cost-sharing mechanism, providers’ knowledge and practical abilities, parental awareness, postpartum discomfort, fathers’ inadequate involvement, and the impact from COVID-19. Conclusion The Safe Neonatal Project pilot experience indicated the feasibility of implementing KMC in more areas of China. Optimising institutional regulations, providing necessary supporting resources and enhancing education and training may help to refine the implementation and scale-up of KMC practice in China.

الإتاحة: https://doi.org/10.1186/s12884-023-05720-4Test

المؤلفون: Zheng, Shuangshuang, Fang, Jianing, Bai, Guannan, He, Xinyu, Hua, Mengdi, Zhu, Bingquan, Chen, Weijun, Dong, Wenhong, Wang, Lei, Huang, Xiaona, Wang, Huishan, Shao, Jie

المساهمون: National Key Research and Development Program of China, National Natural Science Foundation of China

المصدر: BMC Public Health ; volume 23, issue 1 ; ISSN 1471-2458

مصطلحات موضوعية: Public Health, Environmental and Occupational Health

الوصف: Background Nurturing care is necessary for optimal early childhood development. This study aimed to investigate the prevalence of parental risks in rural East China and assess their impacts on early development in children younger than three years old. Methods This community-based cross-sectional survey was conducted among 3852 caregiver-child pairs in Zhejiang Province from December 2019 to January 2020. Children aged 0 to 3 years were recruited from China’s Early Childhood Development Program (ECD). Local child health care providers conducted face-to-face interviews with the primary caregivers. Demographic information of the participants was collected by questionnaire. Each child was screened for parental risk through the Parental Risk Checklist designed by the ECD program. The Ages and Stages Questionnaire (ASQ) was used to identify children with potential developmental delays. Multinomial logistic regression model and linear trend test were applied to assess the association between parental risks and suspected developmental delays. Results Among the 3852 children included in the analyses, 46.70% had at least one parental risk and 9.01% presented suspected developmental delays in any domain of ASQ. Parental risk was statistically associated with the overall suspected developmental delay in young children (Relative Risk Ratio (RRR): 1.36; 95% confidence interval (CI): 1.08, 1.72; P = 0.010) after adjusting potential confounders. Compared with children with no parental risk, children exposed to 3 or more parental risks had 2.59, 5.76, 3.95, and 2.84 times higher risk of the suspected developmental delay in overall ASQ, communication, problem-solving, and personal-social domain, respectively (P values < 0.05). The linear trend tests found that the more parental risks, the higher possibility of developmental delay (P values < 0.05). Conclusions Parental risks are prevalent among children under three years in rural East China, which may increase the risk of developmental delays in children. ...

الإتاحة: https://doi.org/10.1186/s12889-023-15702-yTest

المؤلفون: Zhang, Baonan, Li, Yuzhen, Zhu, Xiaodan, Chen, Zhe, Huang, Xiaona, Gong, Tingjie, Zheng, Weiwang, Bi, Zhenle, Zhu, Chenyang, Qian, Jingyi, Li, Xiaoqiang, Jin, Chunhui

المصدر: Discover Oncology ; volume 14, issue 1 ; ISSN 2730-6011

مصطلحات موضوعية: Cancer Research, Endocrine and Autonomic Systems, Endocrinology, Oncology, Endocrinology, Diabetes and Metabolism

الوصف: Background Chemotherapy is the main treatment strategy for patients with advanced HER2-negative gastric cancer (GC); yet, many patients do not respond well to treatment. This study evaluated the sensitivity of a mini patient-derived xenograft (MiniPDX) animal model in patients with HER2-negative intermediate-advanced GC. Methods In this single-arm, open-label clinical study, we consecutively recruited patients with HER2-negative advanced or recurrent GC from September 2018 to July 2021. Tumor tissues were subjected to MiniPDX drug sensitivity tests for screening individualized anti-tumor drugs; appropriate drug types or combinations were selected based on drug screening results. The primary endpoints were progression-free survival (PFS) and safety, and the secondary endpoints were overall survival (OS) and objective response rate (ORR). Results A total of 17 patients were screened, and 14 eligible patients were included.The median follow-up time was 9 (2–34) months. The median PFS time was 14.1 (2–34) months, the median OS time was 16.9 (2–34) months, ORR was 42.9% (6/14), and DCR was 92.9% (13/14). The most common treatment-related adverse events (TRAE) were fatigue (14 (100%)), anorexia (13 (93%)) and insomnia (12 (86%)), and the most common grade 3 or worse TRAE was fatigue (6 (43%)), and anorexia (6 (43%)). The occurrence rate of myelosuppression, nausea and vomiting, abnormal liver enzymes, and other grade 3–4 chemotherapy adverse reactions were relatively low, and no grade 5 treatment-related adverse events occurred. Conclusion Screening HER2-negative medium-advanced GC/GJC chemotherapy regimens and targeted drugs based on MiniPDX animal models showed good tumor activity and safety.

الإتاحة: https://doi.org/10.1007/s12672-023-00661-yTest

المؤلفون: Xu, Ting, Liao, Shimiao, Huang, Meng, Zhu, Chengming, Huang, Xiaona, Jin, Qile, Xu, Demin, Fu, Chuanhai, Chen, Xiangyang, Feng, Xuezhu, Guang, Shouhong

المساهمون: Ma, Dengke K., the National Key R&D Program of China, the National Natural Science Foundation of China, the Strategic Priority Research Program of the Chinese Academy of Sciences, the Fundamental Research Funds for the Central Universities

المصدر: PLOS Genetics ; volume 19, issue 2, page e1010628 ; ISSN 1553-7404

الوصف: Temperature greatly affects numerous biological processes in all organisms. How multicellular organisms respond to and are impacted by hypothermic stress remains elusive. Here, we found that cold-warm stimuli induced depletion of the RNA exosome complex in the nucleoli but enriched it in the nucleoplasm. To further understand the function and mechanism of cold-warm stimuli, we conducted forward genetic screening and identified ZTF-7, which is required for RNA exosome depletion from nucleoli upon transient cold-warm exposure in C . elegans . ZTF-7 is a putative ortholog of human ZNF277 that may contribute to language impairments. Immunoprecipitation followed by mass spectrometry (IP-MS) found that ZTF-7 interacted with RPS-2, which is a ribosomal protein of the small subunit and participates in pre-rRNA processing. A partial depletion of RPS-2 and other proteins of the small ribosomal subunit blocked the cold-warm stimuli-induced reduction of exosome subunits from the nucleoli. These results established a novel mechanism by which C . elegans responds to environmental cold-warm exposure.

الإتاحة: https://doi.org/10.1371/journal.pgen.1010628Test