المؤلفون: Rohit Mehra, Vikram Patra, Rishi Dhillan, Anuka Sharma, Sonal Kashyap, Garima Rastogi, Love Gupta, Reena Singh, Chirag Chopra, Varun Sharma

المصدر: European Journal of Medical Research, Vol 29, Iss 1, Pp 1-6 (2024)

مصطلحات موضوعية: EFEMP1, Varicose veins, Genotyping, Indians, Medicine

الوصف: Abstract Background Varicose vein is a chronic condition that affects the lower extremities of the human body. Several factors have been implicated in the development of this disease, viz age, gender, weight, height and prolonged standing. Recently, genome-wide studies have identified genetic biomarkers that are associated with varicose veins in different ethnic groups. Such genetic studies are lacking in South Asians specifically in Indians where the prevalence of varicose veins is high, and it is important to replicate these variants in the stated population. The study aimed to replicate the association of genetic variants associated with varicose veins in this target population, which were found to be associated with the other ethnic groups. Methodology The studied cohort is of the Indian population comprising unrelated 104 varicose veins cases and 448 non-varicose vein controls. The samples were genotyped using the Illumina Global Screening Array. Using the genomic data from UK BioBank and 23andMe studied cohorts; eight genetic variants were selected to replicate in our dataset. The allelic association was performed to identify the effective allele and risk was estimated using odds ratio and p-value as level of significance. Multifactor Dimensionality Reduction was used to estimate the cumulative effect of variants in Indians. Result Variant rs3791679 of EFEMP1 was found to be associated with varicose veins in Indians. After observing the association of the EFEMP1 with varicose veins, we further ensued to identify all genetic variants within EFEMP1 to uncover the additional variants associated with this trait. Interestingly, we identified six new variants of EFEMP1 gene that have shown association. Moreover, the cumulative effect of all associated variations was estimated and the risk was 2.7 times higher in cases than controls whereas independently their effect ranges from 0.37–1.58. Conclusion This study identifies EFEMP1 as a potential gene related to the risk of varicose veins in Indians. It also highlights that evaluating the maximum number of variants of a gene rather than focusing solely on replicating single variations offers a more comprehensive and nuanced understanding of the genetic factors contributing to a complex trait like varicose veins.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2047-783XTest

الوصول الحر: https://doaj.org/article/d4a8826a4f4a4322bb049a5b4946e0f4Test

المؤلفون: Sandeep Tripathi, Atul Kansagara, Manohar Nandanwar, Parag R Ghorpade, Anuka Sharma, Vijay Sharma

المصدر: International Journal of Pharmaceutical Sciences and Drug Research. 14:68-77

الوصف: A debilitating complication of diabetes mellitus is diabetic ulcers, which leads to increased overall morbidity in patients. The high growth factor content in Platelet Rich Plasma (PRP) makes it a widely used intervention for the treatment of diabetic foot ulcers. The topical application of Stromal Vascular Fraction (SVF) could possibly enhance wound healing. This study aims at evaluating the efficacy of combining SVF and PRP on wound healing in diabetic rabbit model. Diabetes was induced in New Zealand white rabbits by intravenous injection of 125 mg/Kg Alloxan. After two weeks of alloxan, three 6 mm diameter, full thickness excision wounds were made, on inner side of the right ear pinna. The animals after induction were allocated into 4 groups with [8 Diabetic (treated with SVF+PRP), 4 Diabetic (treated with 10% Povidone Iodine (PI)), 1 non-diabetic (treated with SVF+PRP), 1 non diabetic (treated with 10% PI)]. The effect of combined therapy was evaluated by assessing wound margin closure rate, histo-pathological evaluation, and inflammatory cell infiltration, epithelization of ulcerative region, neo-vascularization, and fibrosis. We observed that the rate of wound closure is enhanced in wounds treated with SVF+PRP as compared to the PI solution. Wound closure and healthy healing were demonstrated by histo-pathological analysis. The analysis clearly indicates that the healing process of PI treated animals is slower than that of SVF + PRP treated animals. In conclusion, based on wound healing assessment and histo-pathological examination, the diabetic rabbits treated with SVF + PRP exhibited early development of granulation tissue and early signs of wound closure as compared to diabetic animals with normal PI dressing

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::646c979122240662cc221c3fbf7ee95fTest
https://doi.org/10.25004/ijpsdr.2022.140110Test

المؤلفون: B. S. Chandrashekar, Kalpana Sarangi, Mushtaque A Mastim, Ashima Bhatia, Manishkumar Shah, Vijay Sharma, Mugdha Gupta, Ranjeet Gutte, Anuka Sharma, Ulka Sakhalkar

المصدر: Cureus. 14(5)

مصطلحات موضوعية: General Engineering

الوصف: Background Growth factors from platelets have been emerging as a revolutionary treatment with the ability to induce cell growth in the skin, which results in retarding and reversing the aging process. Platelet-rich plasma (PRP) allows for greater release of growth factors and biologically active proteins, which in turn activates the cascade of stimulation of neoangiogenesis and collagen production. PRP is used in anti-aging and facial skin rejuvenation in the form of dermal injections and topical application during micro-needling. This study was conducted to assess the safety and efficacy of a topically applied face serum, MYOWNN™ (Wockhardt Ltd., Mumbai, India). MYOWNN™ is an autologous growth factor concentrate that has been made into a topical face serum. Methods Male and female subjects in the age group between 30 and 55 years (both inclusive) with Fitzpatrick skin type III-V who had not taken any oral or topical treatments for at least four weeks before and any platelet-rich plasma (PRP) based facial treatment (injections) at least six months before the study entry were included. MYOWNN™ serum was applied on the face once daily at night, approximately 30 minutes before sleeping preferably, for a total duration of five months. Six parameters, i.e. spots, pores, wrinkles, texture, moisture, and pigmentation, were evaluated at regular intervals with Visage-LS (dermaindia®, Tamil Nadu, India), a face analysis system that gives the live status of these six parameters and is an advanced live status skin detection equipment together with shooting, analyzing, and displaying functions, as well as the subjective analysis, was performed by subjects and physicians using different globally accepted scales like physician's global aesthetic improvement scale (PGAIS), subject's global aesthetic improvement scale (SGAIS), subject satisfaction score (SSS), and wrinkle severity rating scale (WSRS). For analysis, a mixed model for repeated measures was used. The model had change from baseline as the dependent variable visit as a factor and baseline assessment result as a covariate. All primary and secondary efficacy endpoints were analyzed using Modified Intent-to-Treat (mITT) populations. Results Improvement in an average of six anti-aging parameters was observed as early as three months while statistically significant improvement was observed by the end of five months of application. A statistically significant improvement in wrinkles was observed by the end of three months of the application itself. There were no product-related adverse events reported. Conclusions Five months of application of MYOWNN™ serum showed a statistically significant improvement in an average of six parameters of anti-aging and face rejuvenation with a p-value of 0.0150 (5% level of significance (i.e. 0.05) and was also well-tolerated.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7513fcd442bdcd6356d0222bdd8e242bTest
https://pubmed.ncbi.nlm.nih.gov/35747053Test

المؤلفون: SANDEEP TRIPATHI, ATUL KANSAGARA, MANOHAR NANDANWAR, PARAG R GHORPADE, VIJAY SHARMA, ANUKA SHARMA

المصدر: International Journal of Pharmaceutical Sciences and Drug Research. 14:249-255

مصطلحات موضوعية: General Medicine

الوصف: Background: Stromal vascular fraction (SVF) is considered a promising source of stem cells, but potential of treatment of damaged liver has not been fully explored. Aim: In this present study, we sought to evaluate the hepato-regenerative effect of SVF combined with activated platelet rich plasma (aPRP) on carbon tetrachloride (CCl4) induced hepatic injury in Sprague Dawley (SD) rats. Material and Methods: Liver fibrosis was induced using CCl4 in 30 SD rats. Sixteen rats survived and were divided into two groups – control and treated. SVF and aPRP were prepared by standard methods from three normal donor rats. Combination SVF-aPRP was administered intravenously via the lateral tail vein in treated group animals, on Day 0, 7 and 14. Normal saline of same dose volume was administered to the control group. AST and ALT levels were monitored throughout the study duration of 28 days. Gross and histopathological analysis of liver was performed post sacrifice on Day 28. Results: Decrease in the ALT/AST levels over the duration of study was observed in both control and treated groups, with a statistically significant reduction in AST/ALT levels between Control ad Treated groups on Day 28. Gross morphological evaluation, fibrosis and cirrhosis grades also showed reduction between control and treated groups. Conclusion: Observations of enzyme levels, gross and histopathological analyses revealed a reduction in severity of liver fibrosis in the treated group. However, we have identified additional parameters that need to be evaluated to corroborate these research findings.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::b1bf659c60499932b983829852466f59Test
https://doi.org/10.25004/ijpsdr.2022.140215Test

المؤلفون: Sangita Gupta, Ulka Sakhalkar, Avinash Karade, Parag Ghorpade, Yasmeen Sidhu, Anuka Sharma, Manohar Nandanwar, Muftedar Ahmed Patel, Atul Kansagara, Vijay Sharma

المصدر: J Diabetes Metab Disord

مصطلحات موضوعية: Contraction (grammar), integumentary system, business.industry, Endocrinology, Diabetes and Metabolism, Growth factor, medicine.medical_treatment, Inflammation, medicine.disease, Streptozotocin, Diabetes mellitus, Anesthesia, Internal Medicine, Sprague dawley rats, Medicine, medicine.symptom, business, Wound healing, Non diabetic, medicine.drug, Research Article

الوصف: BACKGROUNDS: The investigation of wound healing potential of human GFC (growth factor concentrate) was undertaken in diabetic and non-diabetic rats. Primarily, GFC is the combination of several growth factors present in blood which has potential of wound healing. In present study, WCK-GFC kit, a single step optimized kit was used for obtaining human GFC. METHODS: Diabetes in rats was induced by intraperitoneal single injection of 40 mg/kg streptozotocin (STZ). The full thickness circular wounds of 2 cm(2) area were created using sterilized stainless steel biopsy punch. Non-diabetic wounds were topically treated with 100µL and 300µL of GFC, while diabetic wounds were treated with 300µL of GFC. The standard of care treatment groups were included, wherein the non-diabetic and diabetic wound were topically treated with Nadoxin and Z-AD-G skin cream, respectively. The percentage of wound contraction was measured on weekly intervals. At the end of study duration, tissues from wound were collected for histopathological evaluation. RESULTS: Both diabetic and non-diabetic GFC treated rats exhibited a significantly higher rate of wound contraction on day 8 and 15 compared to normal untreated control group and standard-of-care treated rats. Wound healing was induced by GFC through rapid re-epithelialization. On comparing wound healing with standard-of care agent, the GFC treated wounds demonstrated a faster remodeling phase, a better organization and lower inflammation. CONCLUSIONS: The current study demonstrates that topically applied GFC promotes healing of wounds, with enhanced wound contraction in both non-diabetic and diabetic rats.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f41b7205d92383ad2a545717550388a8Test
https://pubmed.ncbi.nlm.nih.gov/34900810Test

المؤلفون: Anuka Sharma, Shalmoli Bhattacharyya, Arnab Pal, Radhika Srinivasan

المصدر: Molecular Cancer Therapeutics. 18:A055-A055

مصطلحات موضوعية: Cancer Research, Gene knockdown, Cancer, Biology, medicine.disease, Stem cell marker, Metastasis, Oncology, Side population, Cancer stem cell, Cancer cell, Cancer research, medicine, Epithelial–mesenchymal transition

الوصف: Background: Cervical cancer is one of the leading causes of cancer related morbidity and mortality amongst women, especially in developing countries. Recent studies suggest that Cancer stem cells (CSC) are critically involved in regulating the chemo resistant behavior of cancer cells and a relapse of the disease. So targeting CSC-like cells along with the bulk tumor cells can be a promising approach for therapeutics. In this context, we demonstrated that knockdown of E-cadherin gene in cervical cancer cells can generate stem cell-like phenotype and induce epithelial to mesenchymal transition. Interestingly, knockdown of E-cadherin gene in normal keratinocytes did not induce CSC-like cells. Methods: In our study the CSC phenotype cells were isolated by side population (SP) assay from cervical cancer cells and immortalized keratinocytes. The expression of stem cell marker and EMT genes were confirmed by qRT-PCR and immunostainings. Further, we performed SiRNA-mediated knockdown of E-Cadherin (E-Cad) and studied the tumorsphere formation, metastasis and invasion ability of cervical cancer cells. Next, using RTPCR based gene expression analysis and tumorsphere formation we studied the stem–like properties of cancer cells. We also performed dose dependent cell viability assay using clinically relevant drugs and inhibitors to analyze the drug resistance behavior of the tumorspheres (cervical organoids). Results: We found that E-Cad knockdown induces EMT in cervical cancer cells with stem cell-like characteristics along with enhanced tumorsphere formation, metastasis and invasion ability. These cells not only possess CSC-like phenotype at transcriptional and translational level but also functionally as shown by drug resistance behavior studies. Conclusion: Thus, we believe that this can be a promising model to study CSC-like characteristics in-vitro in cervical cancer to target the molecular pathways for therapeutics. Citation Format: Shalmoli Bhattacharyya, Anuka Sharma, Arnab Pal, Radhika Srinivasan. Knockdown of E-cadherin gene in cervical cancer cells: An in vitro model for cancer stem cell phenotype? [abstract]. In: Proceedings of the AACR-NCI-EORTC International Conference on Molecular Targets and Cancer Therapeutics; 2019 Oct 26-30; Boston, MA. Philadelphia (PA): AACR; Mol Cancer Ther 2019;18(12 Suppl):Abstract nr A055. doi:10.1158/1535-7163.TARG-19-A055

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::c73620b58a02ba88b09044c1d9b7ae12Test
https://doi.org/10.1158/1535-7163.targ-19-a055Test