دورية أكاديمية
The role of calcium in the volume regulation of rat lacrimal acinar cells
| العنوان: | The role of calcium in the volume regulation of rat lacrimal acinar cells |
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| المؤلفون: | Speake, T., Douglas, I. J., Brown, P. D. |
| المصدر: | Speake , T , Douglas , I J & Brown , P D 1998 , ' The role of calcium in the volume regulation of rat lacrimal acinar cells ' , Journal of Membrane Biology , vol. 164 , no. 3 , pp. 283-291 . https://doi.org/10.1007/s002329900413Test |
| سنة النشر: | 1998 |
| المجموعة: | The University of Manchester: Research Explorer - Publications |
| مصطلحات موضوعية: | Acetylcholine, Ca2+ signaling, Fura-2, Regulatory volume decrease, Secretion |
| الوصف: | Earlier studies have suggested a role for Ca2+ in regulatory volume decrease (RVD) in response to hypotonic stress through the activation of Ca2+-dependent ion channels (Kotera and Brown, 1993; Park et al., 1994). The involvement of Ca2+ in regulating cell volume in rat lacrimal acinar cells was therefore examined using a video-imaging technique to measure cell volume. The trivalent cation Gd3+ inhibited RVD, suggesting that Ca2+ entry is important and may be via stretch-activated cation channels. However, Fura-2 loaded cells did not show an increase in [Ca2+](i) during exposure to hypotonic solutions, The absence of any changes in [Ca2+](i) resulted from the buffering of cytosolic Ca2+ by Fura-2 during hypotonic shock and therefore inhibition of RVD. The intracellular Ca2+ chelator, BAPTA, also inhibited the RVD response to hypotonic shock. An increase in [Ca2+](i) induced by either acetylcholine or ionomycin, was found to decrease cell volume under isotonic conditions in lacrimal acinar cells. Cell shrinkage was inhibited by tetraethylammonium ion, an inhibitor of Ca2+ activated K+ channels. On the basis of the presented data, we suggest an involvement of intracellular Ca2+ in controlling cell volume in lacrimal acinar cells. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1007/s002329900413 |
| الإتاحة: | https://doi.org/10.1007/s002329900413Test https://research.manchester.ac.uk/en/publications/c12ddb5e-9e70-4842-b014-b2de1b15dca9Test |
| حقوق: | info:eu-repo/semantics/closedAccess |
| رقم الانضمام: | edsbas.EF1AEBB0 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1007/s002329900413 |
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