Characterization of Living Dental Pulp Cells in Direct Contact with Mineral Trioxide Aggregate
| العنوان: | Characterization of Living Dental Pulp Cells in Direct Contact with Mineral Trioxide Aggregate |
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| المؤلفون: | Ryuji Yamamoto, Yasuo Miake, Tamaki Hattori-Sanuki, Takeo Karakida, Noriyasu Hosoya, Risako Chiba-Ohkuma, Yasuo Yamakoshi |
| المصدر: | Cells Volume 9 Issue 10 Cells, Vol 9, Iss 2336, p 2336 (2020) |
| بيانات النشر: | Multidisciplinary Digital Publishing Institute, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Mineral trioxide aggregate, Cell Survival, Swine, Cell, Cell Count, Bone morphogenetic protein, cell chemotaxis, Fluorescence, Article, Cell Line, 03 medical and health sciences, 0302 clinical medicine, stomatognathic system, Transforming Growth Factor beta, dental pulp cells, calcium phosphate crystal, medicine, Animals, Cell adhesion, Aluminum Compounds, lcsh:QH301-705.5, Dental Pulp, Cell Proliferation, Cell chemotaxis, mineral trioxide aggregate, biology, Cell growth, Chemistry, Silicates, fungi, fluorescent labeling, Cell Differentiation, Oxides, 030206 dentistry, General Medicine, Transforming growth factor beta, Calcium Compounds, Cell biology, Drug Combinations, Luminescent Proteins, 030104 developmental biology, medicine.anatomical_structure, Odontoblast, lcsh:Biology (General), Bone Morphogenetic Proteins, biology.protein |
| الوصف: | Mineral trioxide aggregate (MTA) was introduced as a material for dental endodontic regenerative therapy. Here, we show the dynamics of living dental pulp cells in direct contact with an MTA disk. A red fluorescence protein (DsRed) was introduced into immortalized porcine dental pulp cells (PPU7) and cloned. DsRed-PPU7 cells were cultured on the MTA disk and cell proliferation, chemotaxis, the effects of growth factors and the gene expression of cells were investigated at the biological, histomorphological and genetic cell levels. Mineralized precipitates formed in the DsRed-PPU7 cells were characterized with crystal structural analysis. DsRed-PPU7 cells proliferated in the central part of the MTA disk until Day 6 and displayed a tendency to move to the outer circumference. Both transforming growth factor beta and bone morphogenetic protein promoted the proliferation and movement of DsRed-PPU7 cells and also enhanced the expression levels of odontoblastic gene differentiation markers. Mineralized precipitates formed in DsRed-PPU7 were composed of calcium and phosphate but its crystals were different in each position. Our investigation showed that DsRed-PPU7 cells in direct contact with the MTA disk could differentiate into odontoblasts by controlling cell&ndash cell and cell&ndash substrate interactions depending on cell adhesion and the surrounding environment of the MTA. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2073-4409 |
| DOI: | 10.3390/cells9102336 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6f894cb05632e016197c323e74e7608fTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....6f894cb05632e016197c323e74e7608f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20734409 |
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| DOI: | 10.3390/cells9102336 |