DctR contributes to the virulence of avian pathogenic Escherichia coli through regulation of type III secretion system 2 expression
| العنوان: | DctR contributes to the virulence of avian pathogenic Escherichia coli through regulation of type III secretion system 2 expression |
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| المؤلفون: | Chenglin Tao, Shaohui Wang, Jingjing Qi, Chan Ding, Dossêh Jean Apôtre Afayibo, Yaodong Zhang, Tao Li, Zhengfei Yi, Hong Zhu, Mingxing Tian, Shengqing Yu, Yao Wang |
| المصدر: | Veterinary Research Veterinary Research, Vol 52, Iss 1, Pp 1-12 (2021) |
| بيانات النشر: | Springer Science and Business Media LLC, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | animal structures, Veterinary medicine, Virulence, Gene Mutant, Biology, Bacterial Adhesion, Type three secretion system, Microbiology, 03 medical and health sciences, Pathogenic Escherichia coli, SF600-1100, Gene expression, Escherichia coli, Type III Secretion Systems, Transcriptional regulation, Gene, 030304 developmental biology, 0303 health sciences, General Veterinary, 030306 microbiology, Escherichia coli Proteins, Avian pathogenic E. coli, E. coli type III secretion system 2, regulation, biology.organism_classification, virulence, Complementation, DctR, Biofilms, Transcription Factors, Research Article |
| الوصف: | Pathogens could precisely alter their gene expression to facilitate their survival and successful infection. The LuxR family transcriptional regulator DctR (also known as YhiF) was shown to participate in the regulation of acid fitness and adhesion of enterohemorrhagic E. coli (EHEC) O157:H7. Avian pathogenic Escherichia coli (APEC) causes significant economic losses to the poultry industries and also potentially threatens human health. However, the effects of DctR on the fitness and virulence of APEC have not been investigated yet. To assess the function of DctR in APEC, the dctR gene mutant and complemented strains were constructed and biologically characterized. Our results show that inactivation of the dctR gene led to decreased biofilm formation, diminished serum resistance, reduced adherence capacity, attenuated colonization and virulence of APEC in ducks. The altered capacities of the mutant strain were restored by genetic complementation. In addition, we found that DctR positively regulates the expression of E. coli type III secretion system 2 (ETT2) core genes in APEC. The expression of the inflammatory cytokines interleukin (IL)-1β and IL-8 were decreased in HD-11 macrophages infected with the mutant strain compared with the wild-type strain. These observations indicate that regulator DctR contributes to the virulence of APEC through regulation of ETT2 expression. |
| تدمد: | 1297-9716 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c2e504528ec992349130cf9c12987e86Test https://doi.org/10.1186/s13567-021-00970-6Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c2e504528ec992349130cf9c12987e86 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 12979716 |
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