Monoclonal antibody anti-PBP2a protects mice against MRSA (methicillin-resistant Staphylococcus aureus) infections
| العنوان: | Monoclonal antibody anti-PBP2a protects mice against MRSA (methicillin-resistant Staphylococcus aureus) infections |
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| المؤلفون: | Felipe Betoni Saraiva, Ana Caroline Cavalcante de Araújo, Anna Erika Vieira de Araujo, José Procópio Moreno Senna |
| المصدر: | PLoS ONE PLoS ONE, Vol 14, Iss 11, p e0225752 (2019) |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Bacterial Lethality, Physiology, Staphylococcus, Antibiotics, medicine.disease_cause, Biochemistry, Mice, 0302 clinical medicine, Immune Physiology, Pathology and laboratory medicine, Mice, Inbred BALB C, Multidisciplinary, Immune System Proteins, biology, Antimicrobials, Antibodies, Monoclonal, Drugs, Animal Models, Staphylococcal Infections, Medical microbiology, Antibodies, Bacterial, Anti-Bacterial Agents, medicine.anatomical_structure, Experimental Organism Systems, Staphylococcus aureus, 030220 oncology & carcinogenesis, Vancomycin, Medicine, Female, Methicillin-resistant Staphylococcus aureus, Antibody, Pathogens, Anatomy, medicine.drug, Research Article, medicine.drug_class, Science, Immunology, Spleen, Mouse Models, Microbial Sensitivity Tests, Monoclonal antibody, Protective Agents, Research and Analysis Methods, Microbiology, Antibodies, 03 medical and health sciences, Model Organisms, Bacterial Proteins, Microbial Control, medicine, Animals, Penicillin-Binding Proteins, Molecular Biology Techniques, Molecular Biology, Medicine and health sciences, Pharmacology, Biology and life sciences, Bacteria, business.industry, Lethal dose, Organisms, Proteins, Kidneys, Bacteriology, Renal System, Bacterial Load, Microbial pathogens, 030104 developmental biology, biology.protein, Animal Studies, Bacterial pathogens, business, Cloning |
| الوصف: | Methicillin-resistant Staphylococcus aureus (MRSA) is a multidrug-resistant bacterium responsible for serious nosocomial and community-acquired infections worldwide. Since few antibiotics are effective for treating MRSA infections, the development of new therapies is of great importance. Previous studies demonstrated that PBP2a is a target that generates protective antibodies against MRSA. A murine monoclonal antibody (MAb) that recognizes PBP2a from MRSA strains was previously isolated and characterized. In this report, we evaluated the biodistribution of this MAb in blood and tissues, as well as the extent of protection conferred using prophylactic and therapeutic assays compared to vancomycin treatment. Biodistribution was evaluated 12-96 h after MAb administration. It predominantly remained in the serum, but it was also detectable in the kidneys, lungs, and spleen at low concentrations (about 4.5% in the kidneys, 1.9% in the lungs, and 0.7% the spleen) at all observed timepoints. Prophylactic studies in a murine model demonstrated a significant bacterial load reduction in the kidneys of the groups treated with either with IgG (greater than 3 logs) or F(ab')2 (98%) when compared to that of the control groups (untreated). Mice were challenged with a lethal dose, and the survival rate was higher in the treated mice. Treatment with the MAb resulted in a bacterial load reduction in the kidneys similar to that of mice treated with vancomycin, and a MAb/vancomycin combination therapy was also effective. These results demonstrate that an anti-PBP2a MAb may be a promising therapeutic for treating MRSA infections. |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::47fa9caf7a5b9c7abfe5939d6537d534Test https://pubmed.ncbi.nlm.nih.gov/31774881Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....47fa9caf7a5b9c7abfe5939d6537d534 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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