Combined Effect of Midazolam and Bone Morphogenetic Protein-2 for Differentiation Induction from C2C12 Myoblast Cells to Osteoblasts
| العنوان: | Combined Effect of Midazolam and Bone Morphogenetic Protein-2 for Differentiation Induction from C2C12 Myoblast Cells to Osteoblasts |
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| المؤلفون: | Risako Chiba-Ohkuma, Yasuo Yamakoshi, Ryuji Yamamoto, Kazuo Onuma, Hiroshi Kawahara, Keiko Fujii-Abe, Mari M. Saito, Yukihiko Hidaka, Takeo Karakida |
| المصدر: | Pharmaceutics, Vol 12, Iss 3, p 218 (2020) Pharmaceutics Volume 12 Issue 3 |
| بيانات النشر: | MDPI AG, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Cell, Pharmaceutical Science, lcsh:RS1-441, drug repositioning, Bone morphogenetic protein 2, bone, Article, lcsh:Pharmacy and materia medica, 03 medical and health sciences, 0302 clinical medicine, medicine, Bone regeneration, 030304 developmental biology, 0303 health sciences, Chemistry, hydroxyapatite, cell, Cell biology, medicine.anatomical_structure, midazolam, Cell culture, 030220 oncology & carcinogenesis, Alkaline phosphatase, Signal transduction, C2C12, Immunostaining |
| الوصف: | In drug repositioning research, a new concept in drug discovery and new therapeutic opportunities have been identified for existing drugs. Midazolam (MDZ) is an anesthetic inducer used for general anesthesia. Here, we demonstrate the combined effects of bone morphogenetic protein-2 (BMP-2) and MDZ on osteogenic differentiation. An immortalized mouse myoblast cell line (C2C12 cell) was cultured in the combination of BMP-2 and MDZ (BMP-2+MDZ). The differentiation and signal transduction of C2C12 cells into osteoblasts were investigated at biological, immunohistochemical, and genetic cell levels. Mineralized nodules formed in C2C12 cells were characterized at the crystal engineering level. BMP-2+MDZ treatment decreased the myotube cell formation of C2C12 cells, and enhanced alkaline phosphatase activity and expression levels of osteoblastic differentiation marker genes. The precipitated nodules consisted of randomly oriented hydroxyapatite nanorods and nanoparticles. BMP-2+MDZ treatment reduced the immunostaining for both &alpha 1 and &gamma 2 subunits antigens on the gamma-aminobutyric acid type A (GABAA) receptor in C2C12 cells, but enhanced that for BMP signal transducers. Our investigation showed that BMP-2+MDZ has a strong ability to induce the differentiation of C2C12 cells into osteoblasts and has the potential for drug repositioning in bone regeneration. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1999-4923 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::60ce3e2aa785e8c20a54a9fe276f91a6Test https://www.mdpi.com/1999-4923/12/3/218Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....60ce3e2aa785e8c20a54a9fe276f91a6 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19994923 |
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