المؤلفون: Hong Tai Chang, Chao Yi Chen, Ho Yi Tuan-Mu, Ying Jan Wang, Chui Yi Pan, Jin Jia Hu, Hsiu Chuan Lin, How Ran Guo, Chao Lin Chen

المصدر: Scientific Reports
Scientific Reports, Vol 9, Iss 1, Pp 1-17 (2019)

مصطلحات موضوعية: Lipopolysaccharides, Male, 0301 basic medicine, In situ, Scaffold, Lipopolysaccharide, Biocompatibility, Blotting, Western, Silicones, lcsh:Medicine, Article, Rats, Sprague-Dawley, 03 medical and health sciences, chemistry.chemical_compound, Peritoneal cavity, 0302 clinical medicine, In vivo, medicine, Animals, Tissue engineering, lcsh:Science, Autografts, Aorta, Ultrasonography, Multidisciplinary, Tissue Scaffolds, biology, Chemistry, lcsh:R, Elastin, Rats, 030104 developmental biology, medicine.anatomical_structure, Regenerative medicine, biology.protein, Conditioning, lcsh:Q, Vascular Grafting, Collagen, 030217 neurology & neurosurgery, Biomedical engineering

الوصف: Autologous vascular grafts have the advantages of better biocompatibility and prognosis. However, previous studies that implanted bare polymer tubes in animals to grow autologous tubular tissues were limited by their poor yield rates and stability. To enhance the yield rate of the tubular tissue, we employed a design with the addition of overlaid autologous whole blood scaffold containing lipopolysaccharides (LPS). Furthermore, we applied in vivo dynamic mechanical stimuli through cyclically inflatable silicone tube to improve the mechanical properties of the harvested tissues. The effectiveness of the modification was examined by implanting the tubes in the peritoneal cavity of rats. A group without mechanical stimuli served as the controls. After 24 days of culture including 16 days of cyclic mechanical stimuli, we harvested the tubular tissue forming on the silicone tube for analysis or further autologous interposition vascular grafting. In comparison with those without cyclic dynamic stimuli, tubular tissues with this treatment during in vivo culture had stronger mechanical properties, better smooth muscle differentiation, and more collagen and elastin expression by the end of incubation period in the peritoneal cavity. The grafts remained patent after 4 months of implantation and showed the presence of endothelial and smooth muscle cells. This model shows a new prospect for vascular tissue engineering.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c248dd04b821476cd96e5ac3bcc37da1Test
https://doi.org/10.1038/s41598-019-47054-2Test

المؤلفون: Ying Jan Wang, Hui Wen Chiu, Jing Hua Lin, Yi An Chen, Sheng Yow Ho

المصدر: Autophagy. 6:353-365

مصطلحات موضوعية: Adult, Male, Programmed cell death, Fibrosarcoma, Antineoplastic Agents, Apoptosis, Mice, SCID, Biology, Arsenicals, Mice, chemistry.chemical_compound, Arsenic Trioxide, In vivo, Cell Line, Tumor, Autophagy, medicine, Animals, Humans, Cytotoxic T cell, Arsenic trioxide, Molecular Biology, Dose-Response Relationship, Drug, Cell Cycle, Dose-Response Relationship, Radiation, Oxides, Cell Biology, medicine.disease, Gene Expression Regulation, Neoplastic, Cell killing, chemistry, Immunology, Cancer research, HT1080, Neoplasm Transplantation

الوصف: The traditional treatments for fibrosarcoma have limited efficacy. Therefore, new therapeutic strategies and/or new adjuvant drugs still need to be explored. Accumulating evidence indicates that programmed cell death (PCD) is closely related to anticancer therapy. Many studies have shown that tumor cells treated with anticancer drugs experience the induction of type I PCD, apoptosis, and type II PCD, autophagy. In the present study, we investigated the anticancer effects of ionizing radiation (IR) combined with arsenic trioxide (ATO) in human fibrosarcoma cells in vitro and in xenograft tumors in SCID mice in vivo. We found that IR increased the population of HT1080 cells in the G2/M phase in a time-dependent manner within 9 h. IR treatment combined with ATO at this time point induced a significantly prolonged G2/M arrest and consequently enhanced cell death. Furthermore, damage of mitochondria membrane potential could be involved in the underlying mechanisms. The enhanced cytotoxic effect of combined treatment occurred due to the increased induction of more autophagy and apoptosis through the inhibition of Akt and the activation of ERK1/2 signaling pathways in HT1080 cells. The combined treatment of HT1080 cells pretreated with Z-VAD or 3-MA resulted in a significant reduction in AO-positive cells, apoptotic cells and cytotoxicity. In in vivo studies, the combination of IR and ATO significantly reduced the tumor volume in SCID mice that had received a subcutaneous injection of HT1080 cells. The data suggest that a combination of IR and ATO could be a new potential therapeutic strategy for the treatment of fibrosarcoma.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b48b03f3d3c49302583353d622e873c9Test
https://doi.org/10.4161/auto.6.3.11229Test

المؤلفون: Chih Hung Chen, Helen H.W. Chen, Chih Jen Chang, Po Chang Huang, Ying Jan Wang, Sen Tien Tsai, Sheng Yow Ho, How Ran Guo

المصدر: Journal of the Chinese Medical Association, Vol 69, Iss 8, Pp 351-357 (2006)

مصطلحات موضوعية: Adult, Male, Nasopharyngeal neoplasm, Single-nucleotide polymorphism, nasopharyngeal neoplasm, Biology, Polymorphism, Single Nucleotide, Polymorphism (computer science), single nucleotide polymorphism, Genotype, medicine, Humans, Allele, Promoter Regions, Genetic, Aged, Medicine(all), lcsh:R5-920, Tumor Necrosis Factor-alpha, allele, Nasopharyngeal Neoplasms, Promoter, General Medicine, Middle Aged, medicine.disease, Molecular biology, Nasopharyngeal carcinoma, Cancer research, Female, Restriction fragment length polymorphism, tumor necrosis factor-α, lcsh:Medicine (General)

الوصف: Background: Tumor necrosis factor- (TNF-) is a pro-inflammatory cytokine and may act as an endogenous tumor promoter. Single nucleotide polymorphisms (SNPs) of the TNF- gene promoter region have been found to be associated with certain cancers. We conducted a case-control study to evaluate the association between these SNPs and nasopharyngeal carcinoma (NPC). Methods: We used polymerase chain reaction followed by restriction fragment length polymorphism analysis to determine the −308 TNF- promoter genotypes of 89 NPC patients and 360 healthy controls. In 23 NPC patients and 50 controls, we determined the sequence from −1065 to −101 nucleotides of the TNF- gene promoter region to detect SNPs. Results: In comparison with the controls, the NPC patients had higher proportions of men and carriage of IgA antibodies against the capsid antigen of Epstein–Barr virus, but had a similar carrier rate of the −308A allele (odds ratio [OR], 1.2; 95% confidence interval [CI], 0.7–2.0). The carriage of the −308A allele was not associated with the occurrence of NPC in comparison with −308G homozygosity. We also found no significant differences in the distributions of allelic variants of the −1031, −863, −857, and −806 loci of the TNF- promoter region, but observed a lower carrier rate of the novel −806T allele in the NPC patients (OR, 0.3; 95% CI, 0.0–2.9). Conclusion: Allelic variants of the TNF- promoter gene may not be used as biomarkers of susceptibility to NPC. The role of the −806T allele needs to be studied further. [J Chin Med Assoc 2006;69(8):351–357]

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b0a94246c77252818cde1bf2be5caf5cTest
http://www.sciencedirect.com/science/article/pii/S1726490109702722Test

المؤلفون: Ben Zhan Zhu, Ying Jan Wang, Hsiu Min Chen, Bour Jr Wang, Rong Jane Chen

المصدر: PLoS ONE
PLoS ONE, Vol 9, Iss 2, p e89483 (2014)

مصطلحات موضوعية: MAPK/ERK pathway, Male, Necrosis, Metabolite, lcsh:Medicine, Apoptosis, Pharmacology, Mitochondrion, Signal transduction, ERK signaling cascade, Toxicology, Biochemistry, chemistry.chemical_compound, Mice, Molecular cell biology, Immunotoxicology, Nucleic Acids, Signaling in Cellular Processes, Inner mitochondrial membrane, lcsh:Science, Extracellular Signal-Regulated MAP Kinases, Apoptotic Signaling, Cellular Stress Responses, chemistry.chemical_classification, Mice, Inbred ICR, Multidisciplinary, Cell Death, Signaling cascades, Flow Cytometry, Chemistry, medicine.symptom, Research Article, Programmed cell death, Pentachlorophenol, Toxic Agents, Biology, Chemical Biology, medicine, Animals, Reactive oxygen species, lcsh:R, Proteins, Hydroquinones, chemistry, lcsh:Q, Reactive Oxygen Species, Spleen

الوصف: Pentachlorophenol (PCP) has been used extensively as a biocide and a wood preservative and has been reported to be immunosuppressive in rodents and humans. Tetrachlorohydroquinone (TCHQ) is a major metabolite of PCP. TCHQ has been identified as the main cause of PCP-induced genotoxicity due to reactive oxidant stress (ROS). However, the precise mechanisms associated with the immunotoxic effects of PCP and TCHQ remain unclear. The aim of this study was to examine the effects of PCP and TCHQ on the induction of ROS and injury to primary mouse splenocytes. Our results shown that TCHQ was more toxic than PCP and that a high dose of TCHQ led to necrotic cell death of the splenocytes through induction of massive and sudden ROS and prolonged ROS-triggered ERK activation. Inhibition of ROS production by N-acetyl-cysteine (NAC) partially restored the mitochondrial membrane potential, inhibited ERK activity, elevated caspase-3 activity and PARP cleavage, and, eventually, switched the TCHQ-induced necrosis to apoptosis. We suggest that prolonged ERK activation is essential for TCHQ-induced necrosis, and that ROS play a pivotal role in the different TCHQ-induced cell death mechanisms.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5f551edae6c2a672093ef6d5552296a6Test
https://pubmed.ncbi.nlm.nih.gov/24586814Test

المؤلفون: Ying Jan Wang, Ming Der Wu, Sheng Yow Ho, Gwo Fang Yuan, Hui Wen Chiu, Wen Hung Fang, Yen-Lin Chen

المصدر: PLoS ONE, Vol 7, Iss 7, p e40462 (2012)
PLoS ONE

مصطلحات موضوعية: Male, Radiation-Sensitizing Agents, Cancer Treatment, lcsh:Medicine, Radiation Tolerance, Mice, Prostate cancer, Molecular Cell Biology, Drug Discovery, lcsh:Science, Cellular Stress Responses, Multidisciplinary, Cell Death, Prostate Cancer, Endoplasmic Reticulum Stress, Chemistry, Oncology, Medicine, Radiology, Heterocyclic Compounds, 3-Ring, Research Article, Drugs and Devices, Programmed cell death, Drug Research and Development, Radiation Therapy, Mice, Nude, Biology, In vivo, Cell Line, Tumor, Autophagy, medicine, Red yeast rice, Animals, Humans, Nutrition, Biological Products, Endoplasmic reticulum, lcsh:R, Radiobiology, Cancers and Neoplasms, Prostatic Neoplasms, Cancer, medicine.disease, Genitourinary Tract Tumors, Cancer cell, Immunology, Cancer research, lcsh:Q, Medicinal Chemistry, Proto-Oncogene Proteins c-akt

الوصف: Prostate cancer is a very common cancer among males. Traditional treatments for prostate cancer have limited efficacy; therefore, new therapeutic strategies and/or new adjuvant drugs must be explored. Red yeast rice (RYR) is a traditional food spice made in Asia by fermenting white rice with Monascus purpureus Went yeast. Accumulating evidence indicates that RYR has antitumor activity. In this study, PC-3 cells (human prostate cancer cells) were used to investigate the anti-cancer effects of ionizing radiation (IR) combined with monascuspiloin (MP, a yellow pigment isolated from Monascus pilosus M93-fermented rice) and to determine the underlying mechanisms of these effects in vitro and in vivo. We found that IR combined with MP showed increased therapeutic efficacy when compared with either treatment alone in PC-3 cells. In addition, the combined treatment enhanced DNA damage and endoplasmic reticulum (ER) stress. The combined treatment induced primarily autophagy in PC-3 cells, and the cell death that was induced by the combined treatment was chiefly the result of inhibition of the Akt/mTOR signaling pathways. In an in vivo study, the combination treatment showed greater anti-tumor growth effects. These novel findings suggest that the combined treatment could be a potential therapeutic strategy for prostate cancer.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f9e32d77b517c7d474991ca2136fd8b1Test
https://doi.org/10.1371/journal.pone.0040462Test

المؤلفون: Hui Wen Chiu, Ying Jan Wang, Sheng Yow Ho, Yi An Chen

المصدر: PLoS ONE
PLoS ONE, Vol 7, Iss 2, p e31579 (2012)

مصطلحات موضوعية: Male, Time Factors, Cancer Treatment, lcsh:Medicine, Apoptosis, Pharmacology, Toxicology, Radiation Tolerance, Arsenicals, Mice, Phosphatidylinositol 3-Kinases, Prostate cancer, chemistry.chemical_compound, Arsenic Trioxide, Radiation, Ionizing, Molecular Cell Biology, Medicine, Arsenic trioxide, lcsh:Science, Cytotoxicity, Multidisciplinary, Oxides, Signaling Cascades, Tumor Burden, Oncology, Androgens, Drug Therapy, Combination, Research Article, Signal Transduction, Drugs and Devices, Programmed cell death, Mice, Nude, Cell Line, Tumor, LNCaP, Autophagy, Animals, Humans, Biology, Dose-Response Relationship, Drug, business.industry, lcsh:R, Body Weight, Radiobiology, Cancers and Neoplasms, Prostatic Neoplasms, Dose-Response Relationship, Radiation, medicine.disease, Genitourinary Tract Tumors, chemistry, Cell culture, Cancer cell, lcsh:Q, business, Proto-Oncogene Proteins c-akt

الوصف: Prostate cancer is the most common malignancy in men. In the present study, LNCaP (androgen-sensitive human prostate cancer cells) and PC-3 cells (androgen-independent human prostate cancer cells) were used to investigate the anti-cancer effects of ionizing radiation (IR) combined with arsenic trioxide (ATO) and to determine the underlying mechanisms in vitro and in vivo. We found that IR combined with ATO increases the therapeutic efficacy compared to individual treatments in LNCaP and PC-3 human prostate cancer cells. In addition, combined treatment showed enhanced reactive oxygen species (ROS) generation compared to treatment with ATO or IR alone in PC-3 cells. Combined treatment induced autophagy and apoptosis in LNCaP cells, and mainly induced autophagy in PC-3 cells. The cell death that was induced by the combined treatment was primarily the result of inhibition of the Akt/mTOR signaling pathways. Furthermore, we found that the combined treatment of cells pre-treated with 3-MA resulted in a significant change in AO-positive cells and cytotoxicity. In an in vivo study, the combination treatment had anti-tumor growth effects. These novel findings suggest that combined treatment is a potential therapeutic strategy not only for androgen-dependent prostate cancer but also for androgen-independent prostate cancer.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f0837ba3516e48207dbfa9d5910ddcd5Test
https://doi.org/10.1371/journal.pone.0031579Test