The microtubule end-binding protein EB2 is a central regulator of microtubule reorganisation in apico-basal epithelial differentiation
| العنوان: | The microtubule end-binding protein EB2 is a central regulator of microtubule reorganisation in apico-basal epithelial differentiation |
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| المؤلفون: | Benjamin J. Tyrrell, Penny P. Powell, Paul Thomas, Deborah A. Goldspink, Mette M. Mogensen, Jennifer Keynton, Jonathan R Gadsby, Elizabeth K. Lund, Gemma Bellett |
| المصدر: | Journal of Cell Science. |
| بيانات النشر: | The Company of Biologists, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Fetal Proteins, Down-Regulation, Formins, macromolecular substances, Microtubules, Downregulation and upregulation, Microtubule, Cell Line, Tumor, Humans, Microtubule end, Intestinal Mucosa, RNA, Small Interfering, Actin, Microtubule nucleation, Gene knockdown, biology, Binding protein, Microfilament Proteins, Nuclear Proteins, Cell Differentiation, Epithelial Cells, Cell Biology, HCT116 Cells, Cochlea, Cell biology, Actin Cytoskeleton, biology.protein, RNA Interference, Caco-2 Cells, Microtubule-Associated Proteins, Protein Binding |
| الوصف: | Microtubule end-binding (EB) proteins influence microtubule dynamic instability, a process that is essential for microtubule reorganisation during apico-basal epithelial differentiation. Here, we establish for the first time that expression of EB2, but not that of EB1, is crucial for initial microtubule reorganisation during apico-basal epithelial differentiation, and that EB2 downregulation promotes bundle formation. EB2 siRNA knockdown during early stages of apico-basal differentiation prevented microtubule reorganisation, whereas its downregulation at later stages promoted microtubule stability and bundle formation. Interestingly, although EB1 is not essential for microtubule reorganisation, its knockdown prevented apico-basal bundle formation and epithelial elongation. siRNA depletion of EB2 in undifferentiated epithelial cells induced the formation of straight, less dynamic microtubules with EB1 and ACF7 lattice association and co-alignment with actin filaments, a phenotype that could be rescued by inhibition with formin. Importantly, in situ inner ear and intestinal crypt epithelial tissue revealed direct correlations between a low level of EB2 expression and the presence of apico-basal microtubule bundles, which were absent where EB2 was elevated. EB2 is evidently important for initial microtubule reorganisation during epithelial polarisation, whereas its downregulation facilitates EB1 and ACF7 microtubule lattice association, microtubule-actin filament co-alignment and bundle formation. The spatiotemporal expression of EB2 thus dramatically influences microtubule organisation, EB1 and ACF7 deployment and epithelial differentiation. |
| تدمد: | 1477-9137 0021-9533 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5190a5caf51b4b26e950528bc1a120b7Test https://doi.org/10.1242/jcs.129759Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....5190a5caf51b4b26e950528bc1a120b7 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14779137 00219533 |
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