التفاصيل البيبلوغرافية
| العنوان: |
Noncovalent Protein–Pseudorotaxane Assembly Incorporating an Extended Arm Calix[8]arene with α‑Helical Recognition Properties |
| المؤلفون: |
Niamh M. Mockler (10112704), Kiefer O. Ramberg (9339521), Francesca Guagnini (9339515), Colin L. Raston (1964680), Peter B. Crowley (1735465) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biophysics, Biochemistry, Genetics, Molecular Biology, Pharmacology, Inorganic Chemistry, Plant Biology, Computational Biology, Chemical Sciences not elsewhere classified, Physical Sciences not elsewhere classified, supramolecular protein chemistry, b-sclx 8 molecules, assembly, protein recognition, PEG, cyt c, N-terminal α- helix, trimeric b-sclx 8 stack, 16 phenyl rings |
| الوصف: |
Water-soluble, anionic calix[ n ]arenes are useful receptors for protein recognition and assembly. For example, sulfonato-calix[8]arene ( sclx 8 ) can encapsulate proteins and direct their assembly into porous frameworks. In this work, we turned our attention to an “extended arm” calixarene with 16 phenyl rings. We hypothesized that this larger receptor would have increased capacity for protein masking/encapsulation. A cocrystal structure of p -benzyl-sulfonato-calix[8]arene ( b-sclx 8 ) and cytochrome c (cyt c ) revealed a surprising assembly. A pseudorotaxane comprising a stack of three b-sclx 8 molecules threaded by polyethylene glycol (PEG) was bound to the protein. The trimeric b-sclx 8 stack, a tubelike structure with a highly charged surface, mediated assembly via a new mode of protein recognition. The calixarene stack presents four hydrophobic grooves, each of which binds to one cyt c by accommodating the N-terminal α-helix. This unprecedented binding mode suggests new possibilities for supramolecular protein chemistry. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| العلاقة: |
https://figshare.com/articles/journal_contribution/Noncovalent_Protein_Pseudorotaxane_Assembly_Incorporating_an_Extended_Arm_Calix_8_arene_with_Helical_Recognition_Properties/13749691Test |
| DOI: |
10.1021/acs.cgd.0c01717.s001 |
| الإتاحة: |
https://doi.org/10.1021/acs.cgd.0c01717.s001Test |
| حقوق: |
CC BY-NC 4.0 |
| رقم الانضمام: |
edsbas.AA3DB644 |
| قاعدة البيانات: |
BASE |
