Calixarene capture of partially unfolded cytochrome c
| العنوان: | Calixarene capture of partially unfolded cytochrome c |
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| المؤلفون: | Sylvain Engilberge, Martin L. Rennie, Peter B. Crowley |
| المصدر: | FEBS Letters. 593:2112-2117 |
| بيانات النشر: | Wiley, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Models, Molecular, Saccharomyces cerevisiae Proteins, Stereochemistry, Lysine, Biophysics, Supramolecular chemistry, Saccharomyces cerevisiae, Crystal structure, Arginine, Crystallography, X-Ray, Biochemistry, 03 medical and health sciences, chemistry.chemical_compound, Structural Biology, Calixarene, Genetics, Imidazole, Molecular Biology, Protein Unfolding, 030304 developmental biology, 0303 health sciences, Binding Sites, biology, Cytochrome c, Omega loop, 030302 biochemistry & molecular biology, Imidazoles, Cytochromes c, Cell Biology, Electron transport chain, chemistry, biology.protein, Calixarenes |
| الوصف: | Supramolecular receptors such as water-soluble calixarenes are in development as 'molecular glues' for protein assembly. Here, we obtained cocrystals of sulfonato-calix[6]arene (sclx6 ) and yeast cytochrome c (cytc) in the presence of imidazole. A crystal structure at 2.65 A resolution reveals major structural rearrangement and disorder in imidazole-bound cytc. The largest protein-calixarene interface involves 440 A2 of the protein surface with key contacts at Arg13, Lys73, and Lys79. These lysines participate in alkaline transitions of cytc and are part of Ω-loop D, which is substantially restructured in the complex with sclx6 . The structural modification also includes Ω-loop C, which is disordered (residues 41-55 inclusive). These results suggest the possibility of using supramolecular scaffolds to trap partially disordered proteins. |
| تدمد: | 1873-3468 0014-5793 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::990df2aee85679d51d1b079b02caf5feTest https://doi.org/10.1002/1873-3468.13512Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....990df2aee85679d51d1b079b02caf5fe |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18733468 00145793 |
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