Francisella tularensis enters a double membraned compartment following cell-cell transfer
| العنوان: | Francisella tularensis enters a double membraned compartment following cell-cell transfer |
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| المؤلفون: | Shaun Steele, Jason M. Park, Zach Chamberlain, Thomas H. Kawula |
| المصدر: | eLife eLife, Vol 8 (2019) |
| بيانات النشر: | eLife Sciences Publications, Ltd, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Trogocytosis, QH301-705.5, Phagocytosis, Science, 030106 microbiology, Cell, Vacuole, General Biochemistry, Genetics and Molecular Biology, Microbiology, 03 medical and health sciences, medicine, bacterial transfer, trogocytosis, Biology (General), Francisella tularensis, Type VI secretion system, Microbiology and Infectious Disease, General Immunology and Microbiology, biology, Chemistry, General Neuroscience, merocytophagy, cell-cell transfer, General Medicine, biology.organism_classification, Cytosol, 030104 developmental biology, medicine.anatomical_structure, Cytoplasm, Medicine, Other, Research Advance |
| الوصف: | Previously, we found that phagocytic cells ingest bacteria directly from the cytosol of infected cells without killing the initially infected cell (Steele et al., 2016). Here, we explored the events immediately following bacterial transfer. Francisella tularensis bacteria acquired from infected cells were found within double-membrane vesicles partially composed from the donor cell plasma membrane. As with phagosomal escape, the F. tularensis Type VI Secretion System (T6SS) was required for vacuole escape. We constructed a T6SS inducible strain and established conditions where this strain is trapped in vacuoles of cells infected through bacterial transfer. Using this strain we identified bacterial transfer events in the lungs of infected mice, demonstrating that this process occurs in infected animals. These data and electron microscopy analysis of the transfer event revealed that macrophages acquire cytoplasm and membrane components of other cells through a process that is distinct from, but related to phagocytosis. |
| اللغة: | English |
| تدمد: | 2050-084X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a3c95c2788757364e964dbc23f84c0c5Test http://europepmc.org/articles/PMC6499538Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....a3c95c2788757364e964dbc23f84c0c5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 2050084X |
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