Combination of inductive effect of lipopolysaccharide and in situ mechanical conditioning for forming an autologous vascular graft in vivo
| العنوان: | Combination of inductive effect of lipopolysaccharide and in situ mechanical conditioning for forming an autologous vascular graft in vivo |
|---|---|
| المؤلفون: | Hong Tai Chang, Chao Yi Chen, Ho Yi Tuan-Mu, Ying Jan Wang, Chui Yi Pan, Jin Jia Hu, Hsiu Chuan Lin, How Ran Guo, Chao Lin Chen |
| المصدر: | Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-17 (2019) |
| بيانات النشر: | Springer Science and Business Media LLC, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Lipopolysaccharides, Male, 0301 basic medicine, In situ, Scaffold, Lipopolysaccharide, Biocompatibility, Blotting, Western, Silicones, lcsh:Medicine, Article, Rats, Sprague-Dawley, 03 medical and health sciences, chemistry.chemical_compound, Peritoneal cavity, 0302 clinical medicine, In vivo, medicine, Animals, Tissue engineering, lcsh:Science, Autografts, Aorta, Ultrasonography, Multidisciplinary, Tissue Scaffolds, biology, Chemistry, lcsh:R, Elastin, Rats, 030104 developmental biology, medicine.anatomical_structure, Regenerative medicine, biology.protein, Conditioning, lcsh:Q, Vascular Grafting, Collagen, 030217 neurology & neurosurgery, Biomedical engineering |
| الوصف: | Autologous vascular grafts have the advantages of better biocompatibility and prognosis. However, previous studies that implanted bare polymer tubes in animals to grow autologous tubular tissues were limited by their poor yield rates and stability. To enhance the yield rate of the tubular tissue, we employed a design with the addition of overlaid autologous whole blood scaffold containing lipopolysaccharides (LPS). Furthermore, we applied in vivo dynamic mechanical stimuli through cyclically inflatable silicone tube to improve the mechanical properties of the harvested tissues. The effectiveness of the modification was examined by implanting the tubes in the peritoneal cavity of rats. A group without mechanical stimuli served as the controls. After 24 days of culture including 16 days of cyclic mechanical stimuli, we harvested the tubular tissue forming on the silicone tube for analysis or further autologous interposition vascular grafting. In comparison with those without cyclic dynamic stimuli, tubular tissues with this treatment during in vivo culture had stronger mechanical properties, better smooth muscle differentiation, and more collagen and elastin expression by the end of incubation period in the peritoneal cavity. The grafts remained patent after 4 months of implantation and showed the presence of endothelial and smooth muscle cells. This model shows a new prospect for vascular tissue engineering. |
| تدمد: | 2045-2322 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c248dd04b821476cd96e5ac3bcc37da1Test https://doi.org/10.1038/s41598-019-47054-2Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c248dd04b821476cd96e5ac3bcc37da1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
|---|