Cellular transduction of mechanical oscillations in plants by the plasma-membrane mechanosensitive channel MSL10
| العنوان: | Cellular transduction of mechanical oscillations in plants by the plasma-membrane mechanosensitive channel MSL10 |
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| المؤلفون: | Nathalie Leblanc-Fournier, Emmanuel de Langre, Jean-Marie Frachisse, Bruno Moulia, Tiffanie Girault, Marjorie Guichard, Sébastien Thomine, Daniel Tran, Jean-Marc Allain |
| المساهمون: | Agroscope, Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Physique et Physiologie Intégratives de l’Arbre en environnement Fluctuant (PIAF), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Laboratoire d'hydrodynamique (LadHyX), École polytechnique (X)-Centre National de la Recherche Scientifique (CNRS), Mathematical and Mechanical Modeling with Data Interaction in Simulations for Medicine (M3DISIM), Laboratoire de mécanique des solides (LMS), École polytechnique (X)-Mines Paris - PSL (École nationale supérieure des mines de Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-École polytechnique (X)-Mines Paris - PSL (École nationale supérieure des mines de Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Inria Saclay - Ile de France, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria), ANR-09-BLAN-0245,Senzo(2009), ANR-11-BSV7-0010,CAROLS,Canaux ioniques et espèces actives de l'oxygène dans le poil absorbant de légumineuse: rôle dans la symbiose avec Rhizobium(2011), ANR-10-LABX-0040,SPS,Saclay Plant Sciences(2010), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), École polytechnique (X)-MINES ParisTech - École nationale supérieure des mines de Paris, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-École polytechnique (X)-MINES ParisTech - École nationale supérieure des mines de Paris |
| المصدر: | Proc Natl Acad Sci U S A Proceedings of the National Academy of Sciences of the United States of America Proceedings of the National Academy of Sciences of the United States of America, 2020, 118 (1), 7 p. ⟨10.1073/pnas.1919402118⟩ Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2021, 118 (1), 7 p. ⟨10.1073/pnas.1919402118⟩ |
| بيانات النشر: | National Academy of Sciences, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0106 biological sciences, Materials science, Arabidopsis, 01 natural sciences, Mechanotransduction, Cellular, Ion Channels, 03 medical and health sciences, Passive movements, vibration modes, wind, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Mechanotransduction, 030304 developmental biology, mechanotransduction, mechanosensitive channel, 0303 health sciences, Multidisciplinary, Ion Transport, Oscillation, Arabidopsis Proteins, atmsl10 wind, Cell Membrane, Membrane Proteins, Plasma, oscillation, Biological Sciences, Plants, Genetically Modified, Membrane, frequency, ion-channel, Biophysics, Mechanosensitive channels, Transduction (physiology), Mechanoreceptors, 010606 plant biology & botany, Communication channel, Signal Transduction |
| الوصف: | International audience; Plants spend most of their life oscillating around 1–3 Hz due to the effect of the wind. Therefore, stems and foliage experience repetitive mechanical stresses through these passive movements. However, the mechanism of the cellular perception and transduction of such recurring mechanical signals remains an open question. Multimeric protein complexes forming mechanosensitive (MS) channels embedded in the membrane provide an efficient system to rapidly convert mechanical tension into an electrical signal. So far, studies have mostly focused on nonoscillatory stretching of these channels. Here, we show that the plasma-membrane MS channel MscS-LIKE 10 (MSL10) from the model plant Arabidopsis thaliana responds to pulsed membrane stretching with rapid activation and relaxation kinetics in the range of 1 s. Under sinusoidal membrane stretching MSL10 presents a greater activity than under static stimulation. We observed this amplification mostly in the range of 0.3–3 Hz. Above these frequencies the channel activity is very close to that under static conditions. With a localization in aerial organs naturally submitted to wind-driven oscillations, our results suggest that the MS channel MSL10, and by extension MS channels sharing similar properties, represents a molecular component allowing the perception of oscillatory mechanical stimulations by plants. |
| اللغة: | English |
| تدمد: | 0027-8424 1091-6490 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dec2f6ee2389015db281e16dc370b170Test https://europepmc.org/articles/PMC7817121Test/ |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....dec2f6ee2389015db281e16dc370b170 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00278424 10916490 |
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