المؤلفون: Luca, Q., De Marchi, G., Domenis, R., Cabas, N., Guella, S., Paradiso, A., Fabro, C., Beltrami, A. P., De Vita, S., Curcio, F.

المصدر: Scientific Abstracts

الإتاحة: https://doi.org/10.1136/annrheumdis-2023-eular.5645Test

المؤلفون: PROIA, Patrizia, BIANCO, Antonino, Aprile, A, Lo Curcio, F, PALMA, Antonio

المساهمون: Proia, P, Aprile, A, Lo Curcio, F, Bianco, A, Palma, A

مصطلحات موضوعية: aerobic and circuit training,ERE (Endurance-Resistance-Endurance),ECE (Endurance-Circuit-Endurance), Settore BIO/10 - Biochimica

الوصف: The aim of this study was to compare three different methods of training in order to determine the effectiveness on weight loss in relation to body composition in adults. One hundred subjects aged 18 to 38 years, with a BMI equal to or greater than 27 and a body fat percentage greater than 22% for women and 12% for men, were enrolled in this study. They were subdivided into 3 groups that followed a different protocol. Before to start the training protocols was detected the starting level of fitness by a test of maximal oxygen consumption, VO2 max and maximal strength tests that it was detected by one maximal repetition of the major muscle groups; this has allowed to draw up personalized training cards, with specific loads for each participant of the project. However, to detect the health conditions blood tests were made. Particularly, we checked glucose, BUN, total cholesterol, triglycerides, HDL and LDL as well as CK and LDH (markers of health status of the muscles). The three different training protocols are called ERE (Endurance-Resistance-Endurance), ECE (Endurance-Circuit-Endurance) and CIRCUIT TRAINING. The ERE protocol included three parts: the first and the third phase were aerobic in which the heart rate was 65% of the maximum. The central anaerobic phase in which there were the involvement of different areas through the use of muscle strength machines. The intensity of the exercises was maintained at 60-70% of maximal repetitions. The ECE protocol was different just in the central part, in which it required repetitions of free body exercises with an intensity maintained at 50% of maximal repetitions. Obviously, the CIRCUIT TRAINING protocol was the central part of the previous protocol. At the end of the eight weeks of training all the determinations (body composition and blood tests) was made. The results obtained suggest that the optimum methods for the purpose of improvement of the physical form (increase lean body mass at the expense of fat mass) is Endurance-Circuit-Endurance that is the ...

العلاقة: ispartofbook:Sport science for health; 4th National Congress SISMES; numberofpages:1; journal:Sport science for health; http://hdl.handle.net/10447/74353Test

الإتاحة: http://hdl.handle.net/10447/74353Test

المؤلفون: Arlot Bonnemains Y, Veltri A, Di Fiore A, Dainelli M, Curcio F, Ambesi Impiombato FS, BALDINI, ENKE, ULISSE, SALVATORE, D'ARMIENTO, Massimino

المساهمون: Arlot Bonnemains, Y, Baldini, Enke, Veltri, A, Di Fiore, A, Dainelli, M, Curcio, F, Ambesi Impiombato, F, Ulisse, Salvatore, D'Armiento, Massimino

مصطلحات موضوعية: Aurora kinase, Inhibitor, Thyroid, Cancer

الوصف: Anaplastic thyroid cancers (ATC) are aggressive tumors, which exhibit cell cycle misregulations leading to uncontrolled cellular proliferation and genomic instability. They fail to respond to chemotherapeutic agents and radiation therapy and most patients die within few months from the diagnosis. In the present study, we evaluated the in vitro effects on ATC cells of VX-680, an inhibitor of the Aurora serine/threonine kinases involved in the regulation of multiple aspects of chromosome segregation and cytokinesis. The effects of VX-680 on proliferation, apoptosis, soft agar colony formation, cell cycle and ploidy were tested on the ATC derived cell lines CAL-62, 8305C, 8505C and BHT-101. Treatment of the different ATC cells with VX-680 inhibited proliferation in a time- and dose-dependent manner, with the IC50 comprised between 25 nM and 150 nM. The VX-680 significantly impaired the ability of the different cell lines to form colonies in soft agar. Analysis of caspase-3 activity showed that VX-680 induced apoptosis in the different cell lines. CAL-62 cells exposed 12 h to VX-680 showed an accumulation of cells with ≥ 4N DNA content. Time-lapse analysis demonstrated that VX-680 treated CAL-62 cells exit metaphase without dividing. Moreover, histone H3 phosphorylation was abrogated following VX-680 treatment. In conclusion, our data demonstrated that VX-680 is effective in reducing cell growth of different ATC derived cell lines and warrant further investigation to exploit its potential therapeutic value for ATC treatment.

وصف الملف: STAMPA

العلاقة: ispartofbook:JOURNAL OF ENDOCRINOLOGICAL INVESTIGATION; XXVII Giornate Endocrinologiche Pisane; volume:31 (Suppl.to No. 4); firstpage:8; lastpage:8; numberofpages:1; journal:JOURNAL OF ENDOCRINOLOGICAL INVESTIGATION. SUPPLEMENT; http://hdl.handle.net/11573/488370Test

الإتاحة: http://hdl.handle.net/11573/488370Test

المؤلفون: ULISSE, SALVATORE, BALDINI, ENKE, BOCCHINI, SARAH, FUMAROLA, Angela, DE ANTONI, Enrico, D'ARMIENTO, Massimino, TOLLER M, CAVALI A, GUÈHO A, GRAZIANO F. M, CURCIO F, AMBESI IMPIOMBATO F. S, ARLOT BONNEMAINS Y

المساهمون: Ulisse, Salvatore, Baldini, Enke, Toller, M, Cavali, A, Guèho, A, GRAZIANO F., M, Bocchini, Sarah, Fumarola, Angela, Curcio, F, DE ANTONI, Enrico, AMBESI IMPIOMBATO F., S, ARLOT BONNEMAINS, Y, D'Armiento, Massimino

الوصف: Aurora-A kinase has been shown to be deregulated in thyroid cancer cells and tissues (Int J Cancer 119:275-282, 2006). Among the Aurora-A substrates identified, TACC3, a member of the Transforming Acidic Coiled-Coil family, has been shown to play a role in cell cycle progression. Moreover, alterations of TACC3 expression occur in several cancer types. Despite that, its expression in normal or transformed human thyrocytes has not been investigated. In the present study, we first demonstrated the expression of the TACC3 gene in normal human thyrocytes (HTU5) and showed that it is modulated in a cell cycle dependent manner. Experiment of immunoprecipitation from human thyrocytes cell protein extracts demonstrated that Aurora-A and TACC3 interact in vivo, while experiments of indirect immunofluorescence showed that TACC3 and Aurora-A co-localize in the proximity of the centrosome onto the spindle microtubules. We next demonstrated that the levels of TACC3 mRNA and protein were, with respect to HTU5 cells, unchanged in cells derived from a benign thyroid follicular tumor (HTU42), and significantly reduced in cell lines derived from follicular (FTC-133), papillary (B-CPAP) and anaplastic thyroid carcinomas (CAL-62 and 8305C). Moreover, in 16 differentiated human thyroid cancer tissues TACC3 mRNA levels were found, with respect to normal matched tissues, reduced in 56% of cases by 0.50±0.07 fold (p<0.01) and increased in 44% of cases by 1.96±0.35 fold (p<0.05). In the same tissues, a correlation between the expression of the TACC3 and Aurora-A mRNAs could be demonstrated (r=0.717, p<0.01). In conclusions, we demonstrated that TACC3 gene is expressed in human thyroid cells in a cell cycle dependent manner, and that the protein is localized onto the spindle microtubules where it interacts with Aurora-A. Along with the observation that TACC3 and Aurora-A expression varied together in human thyroid cancer tissues, we suggest that these proteins may serve similar functions and that deregulation of one or both gene ...

وصف الملف: STAMPA

العلاقة: XXXII National Congress of the Italian Society of Endocrinology; volume:30, Suppl. to No. 4; firstpage:15; lastpage:15; numberofpages:1; journal:JOURNAL OF ENDOCRINOLOGICAL INVESTIGATION. SUPPLEMENT; http://hdl.handle.net/11573/204090Test

الإتاحة: http://hdl.handle.net/11573/204090Test

المؤلفون: ULISSE, SALVATORE, BALDINI, ENKE, DE ANTONI, Enrico, GIACOMELLI, Laura, D'ARMIENTO, Massimino, Toller, M, Gueho, A, Curcio, F, AMBESI IMPIOMBATO, Fs, ARLOT BONNEMAINS, Y

المساهمون: Ulisse, Salvatore, Baldini, Enke, Toller, M, Gueho, A, Curcio, F, DE ANTONI, Enrico, Giacomelli, Laura, AMBESI IMPIOMBATO, F, ARLOT BONNEMAINS, Y, D'Armiento, Massimino

الوصف: Recentemente è stata dimostrata un’alterata espressione della chinasi mitotica Aurora-A nei tumori differenziati della tiroide. Fra i substrati identificati dell’Aurora-A, TACC3, un membro della famiglia di proteine contenenti il motivo “Transforming Acidic Coiled-Coil”, riveste un ruolo nella progressione del ciclo cellulare e la sua espressione è modificata in differenti tipi di tumore. Nel presente studio abbiamo dimostrato, tramite esperimenti di RT-PCR quantitativa e western blot, l’espressione della TACC3 in tireociti umani normali (HTU5). Inoltre, la deprivazione del siero dal mezzo di coltura, in grado di ridurre la proliferazione cellulare di oltre il 75%, determinava una riduzione dell’espressione della TACC3, mentre l’aggiunta del siero al mezzo di coltura per 24 ore era in grado di ristabilire la proliferazione e l’espressione della TACC3 ai livelli di controllo. Rispetto alle cellule HTU5, l’espressione della TACC3 è risultata invariata in cellule derivate da un adenoma follicolare (HTU42), mentre era significativamente ridotta in linee cellulari derivate da carcinoma follicolare (FTC-133), papillare (B-CPAP) e anaplastico (CAL-62 e 8305C) della tiroide. Inoltre, l’analisi di 16 tumori differenziati della tiroide ha rivelato che i livelli di mRNA per la TACC3 erano, rispetto al tessuto tiroideo normale dello stesso paziente, ridotti nel 56% dei casi di 0.50±0.07 volte (p<0.01) e aumentati nel 44% dei casi di1.96±0.35 volte (p<0.05). Inoltre, l’analisi dei livelli di mRNA per la chinasi Aurora-A negli stessi tessuti ha rivelato la presenza di una correlazione positiva fra l’espressione della TACC3 e dell’Aurora-A (r=0.717, p<0.01). Infine, esperimenti di immunoprecipitazione hanno altresì dimostrato che TACC3 e Aurora-A interagiscono in vivo nelle cellule tiroidee umane ed esperimenti di immunofluorescenza evidenziavano la co-localizzazione delle due proteine sul fuso mitotico. In conclusione, i risultati dimostrano per la prima volta che TACC3 è espressa nei tireociti umani normali e che ...

وصف الملف: STAMPA

العلاقة: ispartofbook:Libro degli Abstract - XXIV Giornate Italiane Della Tiroide; XXIV Giornate Italiane Della Tiroide; firstpage:41; lastpage:41; numberofpages:1; http://hdl.handle.net/11573/419022Test

الإتاحة: http://hdl.handle.net/11573/419022Test

المؤلفون: ULISSE, SALVATORE, BALDINI, ENKE, GIACOMELLI, Laura, D'ARMIENTO, Massimino, DELCROS J. G, TOLLER M., CURCIO F., AMBESI IMPIOMBATO F. S., ARLOT BONNEMAINS Y.

المساهمون: Ulisse, Salvatore, DELCROS J., G, Baldini, Enke, Toller, M., Curcio, F., Giacomelli, Laura, AMBESI IMPIOMBATO, F. S., ARLOT BONNEMAINS, Y., D'Armiento, Massimino

مصطلحات موضوعية: Aurora kinase, cell malignant transormation, cancer

الوصف: The Aurora kinases are involved in the regulation of cell cycle progression and alterations in their expression have been shown to associate with cell malignant transformation. In the present study we demonstrated that human thyrocytes express all three Aurora kinases (A, B and C) at both protein and mRNA level and this expression is cell cycle-regulated. An increase in the protein level of the three kinases was found, with respect to normal human thyrocytes (HTU5), in the human cell lines derived from follicular (FTC-133), papillary (B-CPAP) and anaplastic (8305C) thyroid carcinomas, but not in cells derived from a follicular adenoma (HTU42). These observations were mirrored in RT-PCR experiments for Aurora-A and B. In contrast, Aurora-C mRNA levels were not significantly different among the different cell types analyzed, suggesting that post-transcriptional mechanism(s) modulate its expression. The expression at the protein level of all three Aurora kinases was significantly higher in 3 thyroid papillary carcinomas with respect to normal matched tissues obtained from the same patients. Similar modifications, at the mRNA level, could be observed in 7 papillary carcinoma tissues for Aurora-A and B, but not for Aurora-C. In conclusion, we demonstrated that normal human thyrocytes express all three members of the Aurora kinase family and their expression is amplified in malignant thyroid cell lines and tissues. These results suggest that the Aurora kinases may play a relevant role in malignant thyroid cancers, and may represent a putative therapeutic target for thyroid neoplasms.

وصف الملف: STAMPA

العلاقة: ispartofbook:31st Annual Meeting of the European Thyroid Association - ABSTRACT BOOK; 31st Annual Meeting of the European Thyroid Association; firstpage:26; lastpage:26; numberofpages:1; http://hdl.handle.net/11573/419163Test

الإتاحة: http://hdl.handle.net/11573/419163Test

المؤلفون: ULISSE, SALVATORE, Delcros J. G, Toller M, BALDINI, ENKE, Cavaliere R, Attanasio D, Curcio F, Ambesi Impiombato FS, Arlot Bonnemains Y, D'ARMIENTO, Massimino, COLETTA, IOLANDA

المساهمون: Ulisse, Salvatore, Delcros, J. G., Toller, M, Baldini, Enke, Cavaliere, R, Attanasio, D, Curcio, F, Ambesi Impiombato, F, Arlot Bonnemains, Y, D'Armiento, Massimino, Coletta, Iolanda

مصطلحات موضوعية: Aurora kinase, Thyroid, Tumor

الوصف: Members of the Aurora kinase family are involved in the regulation of mitosis, and alterations in their expression have been associated with malignant transformation. Despite that, few information are available regarding the expression of these proteins in normal or transformed human thyrocytes. Thus, we evaluated, by means of western blot (WB), real-time PCR (RT-PCR) and immunofluorescence (IF), the expression and cellular localization of all members of the Aurora family (A, B and C) in human cell lines derived from normal thyrocytes (HTU5), follicular adenoma (HTU42), follicular (FTC-133), papillary (B-CPAP) and anaplastic (8305C) carcinomas. The different cell types, cultured in the appropriate culture media, were used to prepare total RNA or protein cell extracts or fixed for IF experiments. The WB and RT-PCR analysis demonstrated a low level of expression of all kinases in normal thyrocytes. Similarly, a low expression was present in the HTU42 cells. On the contrary, WB experiments showed a remarkable increase (P<0.01) of the three kinases in all the thyroid carcinoma cell lines. This feature was confirmed by the RT-PCR analysis, showing an increased expression (P<0.01) of Aurora A and B mRNAs, ranging from 3 to 7 fold with respect to the HTU5 cells, in the thyroid carcinoma cell lines. Interestingly, for Aurora C no significant changes in the mRNA levels were observed, suggesting the presence of post-transcriptional mechanism(s) controlling its expression. The IF experiments showed that Aurora A localizes at the centrosome of mitotic cells while Aurora B is located in the centromere of metaphase chromosomes, in the spindle midzone during anaphase and in the midbody during cytokinesis. In conclusions, we demonstrated the expression of all members of the Aurora family in normal human thyrocytes, and proved that their overexpression characterize malignant, but not benign, thyroid tumor derived cell lines.

وصف الملف: STAMPA

العلاقة: XXXI Congresso Nazionale della Società Italiana di Endocrinologia; volume:28 (Suppl.to No. 4); firstpage:8; lastpage:8; numberofpages:1; journal:JOURNAL OF ENDOCRINOLOGICAL INVESTIGATION. SUPPLEMENT; http://hdl.handle.net/11573/488356Test

الإتاحة: http://hdl.handle.net/11573/488356Test

المؤلفون: CASALINO, Giuseppe, CURCIO F., MEMOLA CAPECE MINUTOLO F.

المساهمون: Casalino, Giuseppe, Curcio, F., MEMOLA CAPECE MINUTOLO, F.

العلاقة: ICPR 18th. International Conference on Production Research.; http://hdl.handle.net/11589/21913Test

الإتاحة: http://hdl.handle.net/11589/21913Test

المؤلفون: BALDINI, ENKE, ULISSE, SALVATORE, D'ARMIENTO, Massimino, Toller M, Marchioni E, Trimboli P, Graziano FM, Curcio F, Ambesi Impiombato FS

المساهمون: Baldini, Enke, Toller, M, Marchioni, E, Trimboli, P, Graziano, Fm, Curcio, F, Ulisse, Salvatore, Ambesi Impiombato, F, D'Armiento, Massimino

مصطلحات موضوعية: Urokinase plasminogen activator, Human, Thyroid, Tumor, Cell lines

الوصف: The plasmin activating system has been shown to play a key role in growth and metastasis formation of many malignant tumors. However, informations regarding the expression of the different components of this system in thyroid tumors are limited. In the present study, we investigated the expression of the urokinase and tissue-type plasminogen activators (uPA and tPA), the uPA receptor (uPAR) and the two PAs inhibitors, PAI-1 and PAI-2 in normal (HTU5), follicular adenoma (HTU42), follicular carcinoma (FTC-133), papillary carcinoma (B-CPAP) and two anaplastic carcinoma (CAL-62 and 8305C) derived cell lines. To evaluate PAs activity, the different cell lines have been cultured for 24 h in absence of serum, after which surnatants have been analyzed by zymography, while total RNA was extracted from the different cell types to analyze the expression of the different genes by RT-PCR. The zymography results showed that the HTU5 and HTU42 cell lines secrete low levels of uPA but not tPA. The uPA activity was augmented in the surnatants of all carcinoma derived cell lines. In addition, the B-CPAP and the CAL62 cells also showed a low molecular weight uPA activity. However, RT-PCR analysis revealed the presence of uPA mRNA in all the cells analyzed without significant changes between the different lines, suggesting the occurrence of post-transcriptional mechanism(s) responsible for the higher uPA activity observed in all malignant thyroid tumors derived cell lines. The uPAR mRNA was also detected in all cell lines without significant modifications between them. Of the two PAIs, PAI-1 mRNA was equally expressed in all cell lines, while PAI-2 mRNA was absent in the CAL62 and reduced in the B-CPAP and FTC-133 cell lines. In conclusion, the data reported demonstrated a strong increase of uPA activity in all malignant thyroid tumor cell lines with respect to normal human thyrocytes and to benign follicular adenoma cells, and that post-transcriptional mechanism(s) are responsible for the increased enzyme activity. Moreover, our ...

وصف الملف: STAMPA

العلاقة: XXIV Giornate Endocrinologiche Pisane; volume:27 (Suppl.to No. 5); firstpage:2; lastpage:2; numberofpages:1; journal:JOURNAL OF ENDOCRINOLOGICAL INVESTIGATION. SUPPLEMENT; http://hdl.handle.net/11573/488333Test

الإتاحة: http://hdl.handle.net/11573/488333Test

المؤلفون: ULISSE, SALVATORE, BALDINI, ENKE, D'ARMIENTO, Massimino, Arlot Bonnemains Y, Delcros Y. G, Toller M, Trimboli P, Graziano FM, Curcio F, Ambesi Impiombato FS

المساهمون: Ulisse, Salvatore, Arlot Bonnemains, Y, Delcros Y., G, Baldini, Enke, Toller, M, Trimboli, P, Graziano, Fm, Curcio, F, Ambesi Impiombato, F, D'Armiento, Massimino

مصطلحات موضوعية: Aurora chinasi, Tiroide, Tumori, Trasformazione maligna

الوصف: I tre membri della famiglia delle chinasi Aurora (A, B e C) sono fisiologicamente coinvolti nella regolazione della fase mitotica del ciclo cellulare, in cui controllano la segregazione cromosomica e la citocinesi. Inoltre, numerose evidenze sperimentali ne suggeriscono un ruolo nella progressione tumorale e sono attualmente oggetto di studio quali possibili bersagli terapeutici per la cura di differenti tipi di tumore. Nel presente studio abbiamo analizzato l’espressione delle tre chinasi Aurora in una linea cellulare derivata da tireociti umani normali (HTU5) e in linee cellulari ottenute da differenti istotipi di tumore tiroideo umano, fra cui un adenoma follicolare (HTU42), un carcinoma follicolare (FTC-133), un carcinoma papillare (B-CPAP) ed uno anaplastico (8305C). I diversi tipi cellulari sono stati coltivati in appropriati terreni di coltura e quindi utilizzati per la preparazione di RNA totale con il metodo del guanidinio tiocianato o lisati in un opportuno tampone, in presenza di inibitori delle proteasi, per la preparazione degli estratti proteici. L’espressione delle tre chinasi è stata valutata tramite RT-PCR semiquantitativa e “real-time”, e tramite esperimenti di “Western blot” utilizzando anticorpi specifici per le tre chinasi e per la -actina come controllo interno. I risultati ottenuti hanno dimostrato una bassa espressione delle Aurora A, B e C nei tireociti umani normali sia a livello di proteina che di mRNA. L’espressione delle tre chinasi non si modificava sostanzialmente nelle cellule HTU42, mentre i livelli delle proteine erano aumentati (p0.01) in tutte le linee cellulari derivate dai differenti carcinomi tiroidei. Tale aumento era confermato a livello di mRNA, con incrementi variabili dalle 3 alle 10 volte rispetto alle cellule HTU5 per l’Aurora A e B. I livelli di mRNA per l’Aurora C non risultavano aumentati nelle cellule derivate dai differenti istotitpi di carcinoma tiroideo, suggerendo per essa un controllo post-trascrizionale dell’espressione. In conclusione, i nostri risultati ...

وصف الملف: STAMPA

العلاقة: ispartofbook:Atti XXII Giornate Italiane della Tiroide; XXII Giornate Italiane della Tiroide; firstpage:44; lastpage:44; numberofpages:1; http://hdl.handle.net/11573/488346Test

الإتاحة: http://hdl.handle.net/11573/488346Test