Sequence and structure of defective interfering RNAs associated with cucumber necrosis virus infections
| العنوان: | Sequence and structure of defective interfering RNAs associated with cucumber necrosis virus infections |
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| المؤلفون: | D'Ann Rochon, Renée L. Finnen |
| المصدر: | Journal of General Virology. 74:1715-1720 |
| بيانات النشر: | Microbiology Society, 1993. |
| سنة النشر: | 1993 |
| مصطلحات موضوعية: | Genetics, Tombusvirus, Base Sequence, genetic structures, biology, Molecular Sequence Data, Nucleic acid sequence, Defective Viruses, RNA, biology.organism_classification, Virology, Molecular biology, eye diseases, Long non-coding RNA, Virus, Plant Viruses, Complementary DNA, Mutation, RNA, Viral, Coding region, sense organs, Small nucleolar RNA |
| الوصف: | The presence of symptom-attenuating defective interfering (DI) RNAs in a laboratory culture of cucumber necrosis tombusvirus (CNV) was confirmed. Sequencing of cDNA clones of these DI RNAs revealed that CNV DI RNAs retained sequences from the CNV 5'-untranslated and 3'-terminal regions as well as a portion of the coding region for the 92K protein. Similar sequence arrangements were also observed in symptom-attenuating DI RNAs generated de novo from synthetic wild-type CNV transcripts. A comparison of the sequences and biological activities of these CNV DI RNAs is presented. In co-infections of synthetic wild-type CNV and CNV DI RNAs, prominent RNA species of a higher M(r) than the DI RNA used in the co-infection were found. The possible nature of these RNA species is discussed. |
| تدمد: | 1465-2099 0022-1317 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d41ca99f0f6766588cdf9008be0ff0a8Test https://doi.org/10.1099/0022-1317-74-8-1715Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....d41ca99f0f6766588cdf9008be0ff0a8 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14652099 00221317 |
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