Identification of Three Novel Splicing Variants and Expression Analysis of Chicken GPR1 Gene
| العنوان: | Identification of Three Novel Splicing Variants and Expression Analysis of Chicken GPR1 Gene |
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| المؤلفون: | Yan Wang, Xiaoling Zhao, Qihai Xiao, Huadong Yin, Diyan Li, Zhang Xueyou, Qing Zhu, Kai Tian |
| المصدر: | BioMed Research International, Vol 2017 (2017) BioMed Research International |
| بيانات النشر: | Hindawi Limited, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Article Subject, lcsh:Medicine, Biology, General Biochemistry, Genetics and Molecular Biology, Receptors, G-Protein-Coupled, 03 medical and health sciences, Ovarian Follicle, Complementary DNA, Coding region, Animals, Protein Isoforms, Tissue Distribution, Gene, Regulation of gene expression, Messenger RNA, General Immunology and Microbiology, Alternative splicing, lcsh:R, Gene Expression Regulation, Developmental, General Medicine, Molecular biology, Gene expression profiling, Alternative Splicing, 030104 developmental biology, Adipose Tissue, RNA splicing, Female, Chickens, Research Article |
| الوصف: | GPR1is a G protein-coupled receptor that plays critical roles in eukaryotic cells: typically, response to glucose stimulation, lipid accumulation, and transmitting nutrition signals to cAMP pathway. However, the alternative splicing of theGPR1gene and its expression pattern in chicken tissues and ovarian follicles were unknown. In our current study, we used RACE-PCR to identify threeGPR1variants, including the full-length variant (GPR1-va1) and two alternatively spliced variants (GPR1-va2,GPR1-vb). Quantitative real-time PCR examined the expression pattern ofGPR1mRNA in chicken tissues and ovarian follicles. The result reveals that the coding sequence of the three variants cDNA is 1053, 1053, and 627 bp in length, encoding 350, 350, and 208 amino acids, respectively. The three variants ofGPR1show similar tissue distributions;GPR1expression was abundant in the abdominal fat, lung, and heart. With the follicular development, the expression ofGPR1gene gradually increased, andGPR1-va1andGPR1-va2spliced variants expression in F2 were significantly higher than in F5, F4, and prehierarchical follicles (P<0.05). Taken together, we found three novel variants ofGPR1, and the results ofGPR1expression profiling in adipose tissues and ovarian follicles suggest thatGPR1may play a significant role in the lipid accumulation and progression of follicular development. |
| وصف الملف: | text/xhtml |
| اللغة: | English |
| تدمد: | 2314-6141 2314-6133 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d37d85c09b585b928daa9b53c11e0530Test https://doaj.org/article/ee42dd9efdb2461cb71488d5f6c48514Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....d37d85c09b585b928daa9b53c11e0530 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 23146141 23146133 |
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