N-hexacosanol prevents diabetes-induced rat ileal dysfunction without qualitative alteration of the muscarinic receptor system
| العنوان: | N-hexacosanol prevents diabetes-induced rat ileal dysfunction without qualitative alteration of the muscarinic receptor system |
|---|---|
| المؤلفون: | Keisuke Satoh, Yukako Kinoshita, Naho Narimatsu, Motoaki Saito, Hiroto Suzuki, Itaru Satoh, Yoshie Hisadome, Emi Kazuyama, Masashi Yamada, Shinichi Okada |
| المصدر: | Biomedical Research. 28:267-273 |
| بيانات النشر: | Biomedical Research Press, 2007. |
| سنة النشر: | 2007 |
| مصطلحات موضوعية: | Male, medicine.medical_specialty, Carbachol, Ileum, Muscarinic Antagonists, General Biochemistry, Genetics and Molecular Biology, Diabetes Mellitus, Experimental, Rats, Sprague-Dawley, chemistry.chemical_compound, Internal medicine, Muscarinic acetylcholine receptor, medicine, Methoctramine, Animals, Ileal Diseases, Muscarinic acetylcholine receptor M3, General Medicine, Muscarinic acetylcholine receptor M1, Receptors, Muscarinic, Pirenzepine, Rats, Atropine, Endocrinology, medicine.anatomical_structure, chemistry, Fatty Alcohols, Gastrointestinal Motility, medicine.drug |
| الوصف: | We evaluated the effects of N-hexacosanol, a cyclohexenonic long-chain fatty alcohol, on muscarinic receptors in diabetic rat ileal dysfunction. Eight-week-old male SD rats were divided into four groups. After induction of diabetes (streptozotocin 50 mg/kg, i.p.), three groups were maintained for eight weeks with treatment by N-hexacosanol (0, 2 or 8 mg/kg, s.c. every day). Ileum function was investigated by organ bath studies using carbachol and KCl, and the expression levels of muscarinic M(2) and M(3) receptors were investigated by real-time polymerase chain reaction. Various concentrations of subtype-selective muscarinic antagonists, i.e., atropine (non-selective), pirenzepine (M(1) selective), methoctramine (M(2) selective), and 4-diphenylacetoxy-N-methylpiperidine methiodide (4-DAMP, M(1)/M(3) selective), were used in this study. In the presence and absence of these antagonists, contractile response curves to increasing concentrations of carbachol were investigated. Treatment with N-hexacosanol did not alter the diabetic status of the rats, but did significantly prevent the carbachol-induced hypercontractility in diabetic rat ileum. Estimation of the pA(2) values for atropine, pirenzepine, methoctramine, and 4-DAMP indicated that the carbacholinduced contractile response in the ileum is mainly mediated through the muscarinic M(3) receptor subtype in all groups. Furthermore, N-hexacosanol significantly prevented the diabetes-induced up-regulation of intestinal muscarinic M(2) and M(3) receptor mRNAs in streptozotocin-diabetic rats. Our data indicated that N-hexacosanol exerts preventive effects with respect to carbachol-induced hypercontractility in the diabetic rat ileum without qualitative alteration of the muscarinic receptor system. |
| تدمد: | 1880-313X 0388-6107 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0e0cead209ce8366c825f799ba393a74Test https://doi.org/10.2220/biomedres.28.267Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....0e0cead209ce8366c825f799ba393a74 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1880313X 03886107 |
|---|