Investigations into a putative role for the novel BRASSIKIN pseudokinases in compatible pollen-stigma interactions in Arabidopsis thaliana
| العنوان: | Investigations into a putative role for the novel BRASSIKIN pseudokinases in compatible pollen-stigma interactions in Arabidopsis thaliana |
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| المؤلفون: | Nethangi Udugama, Jianfeng Xu, Baoxiu Qi, Daphne R. Goring, Hyun Kyung Lee, Jennifer Doucet |
| المصدر: | BMC Plant Biology BMC Plant Biology, Vol 19, Iss 1, Pp 1-18 (2019) |
| بيانات النشر: | Springer Science and Business Media LLC, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, RM, Arabidopsis, RV, Germination, Pollen Tube, Plant Science, Biology, 01 natural sciences, Genome, 03 medical and health sciences, Receptor-like cytoplasmic kinase, Palmitoylation, lcsh:Botany, Pollen adhesion, Gene, Phylogeny, Genetics, Pseudokinase, biology.organism_classification, Signaling, Stop codon, lcsh:QK1-989, Stigma, 030104 developmental biology, Brassicaceae, Pollen, Pollen tube, Compatible pollen, Lipid modification, Research Article, 010606 plant biology & botany |
| الوصف: | BackgroundIn the Brassicaceae, the early stages of compatible pollen-stigma interactions are tightly controlled with early checkpoints regulating pollen adhesion, hydration and germination, and pollen tube entry into the stigmatic surface. However, the early signalling events in the stigma which trigger these compatible interactions remain unknown.ResultsA set of stigma-expressed pseudokinase genes, termedBRASSIKINs(BKNs), were identified and found to be present in only core Brassicaceae genomes. InArabidopsis thalianaCol-0,BKN1displayed stigma-specific expression while theBKN2gene was expressed in other tissues as well. CRISPR deletion mutations were generated for the two tandemly linkedBKNs, and very mild hydration defects were observed for wild-type Col-0 pollen when placed on thebkn1/2mutant stigmas. In further analyses, the predominant transcript for the stigma-specificBKN1was found to have a premature stop codon in the Col-0 ecotype, but a survey of the 1001Arabidopsisgenomes uncovered three ecotypes that encoded a full-length BKN1 protein. Furthermore, phylogenetic analyses identified intact BKN1 orthologues in the closely related outcrossingArabidopsisspecies,A. lyrataandA. halleri. Finally, the BKN pseudokinases were found to be plasma-membrane localized through the dual lipid modification of myristoylation and palmitoylation, and this localization would be consistent with a role in signaling complexes.ConclusionIn this study, we have characterized the novel Brassicaceae-specific family ofBKNpseudokinase genes, and examined the function ofBKN1andBKN2in the context of pollen-stigma interactions inA. thalianaCol-0. Additionally, premature stop codons were identified in the predicted stigma specificBKN1gene in a number of the 1001A. thalianaecotype genomes, and this was in contrast to the out-crossingArabidopsisspecies which carried intact copies ofBKN1. Thus, understanding the function ofBKN1in other Brassicaceae species will be a key direction for future studies. |
| وصف الملف: | application/pdf |
| تدمد: | 1471-2229 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4b0556a3b8456ba170865d7c3a85ac5fTest https://doi.org/10.1186/s12870-019-2160-9Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....4b0556a3b8456ba170865d7c3a85ac5f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14712229 |
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