التفاصيل البيبلوغرافية
| العنوان: |
Characterization and recruitment of plasmacytoid dendritic cells in synovial fluid and tissue of patients with chronic inflammatory arthritis |
| المؤلفون: |
Lande R., Giacomini E., Serafini B., Rosicarelli B., Sebastiani G. D., Minisola G., Riccieri V., Valesini G., Coccia E. M., TARANTINO, UMBERTO |
| المساهمون: |
Lande, R, Giacomini, E, Serafini, B, Rosicarelli, B, Sebastiani, Gd, Minisola, G, Tarantino, U, Riccieri, V, Valesini, G, Coccia, Em |
| بيانات النشر: |
American Association of Immunologists |
| سنة النشر: |
2004 |
| المجموعة: |
Universitá degli Studi di Roma "Tor Vergata": ART - Archivio Istituzionale della Ricerca |
| مصطلحات موضوعية: |
alpha interferon, CD123 antigen, chemokine, chemokine receptor CXCR3, chemokine receptor CXCR4, CXCL11 chemokine, gamma interferon inducible protein 10, Myxovirus resistance protein A, stromal cell derived factor 1, unclassified drug, adult, aged, article, cell isolation, cell maturation, chemotaxi, chronic arthriti, clinical article, controlled study, dendritic cell, female, flow cytometry, human, human cell, immune response, immunohistochemistry, immunopathogenesi, immunophenotyping, immunoregulation, interferon production |
| الوصف: |
Dendritic cells (DCs) are thought to play a key role in driving the immunopathogenic response underlying chronic inflammatory arthritis. In this study, we have examined the presence and phenotype of plasmacytoid DCs (pDCs) in the synovial fluids (SF) of patients with rheumatoid arthritis (RA),,psoriatic arthritis (PA), and osteoarthritis (OA) and determined the chemotactic properties of SF from these patients toward pDCs. Flow cytometry analysis showed that the percentage of pDCs, identified as a population of Lin(-)CD123(++) cells, is 4- to 5-fold higher in RA SF and PA SIT than in OA SF. The morphological and immunophenotypic characterization of pDCs isolated from PA and RA SF indicates that they are in an immature state, most likely due to inhibitory factors present in RA SF, but are still able to undergo maturation when exposed ex vivo to viral agent or unmethylated DNA. CD123(+) and BDCA2(+) pDCs were detected by immunohistochemistry in RA synovial tissue in which expression of the IFN-alpha-inducible protein MxA was also found, suggesting production of type I IFN by maturing pDCs. We also show that CXCR3 and CXCR4 are expressed by both blood-derived pDCs and pDCs isolated from RA and PA SF and that CXCL-10, CXCL-11, and CXCL-12 present in RA and PA SIT stimulate chemotaxis of blood-derived pDCs. Altogether, these findings suggest that chemokine-driven recruitment of pDCs from the blood to the inflamed synovium could be important in the regulation of the immune response in chronic inflammatory arthritis. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
Italian |
| العلاقة: |
volume:173; issue:4; firstpage:2815; lastpage:2824; journal:JOURNAL OF IMMUNOLOGY; http://hdl.handle.net/2108/36473Test; PMID: 15295000 |
| الإتاحة: |
http://hdl.handle.net/2108/36473Test |
| رقم الانضمام: |
edsbas.3A7EE127 |
| قاعدة البيانات: |
BASE |
