المؤلفون: Jaisoo Kim, Dhiraj Kumar Chaudhary, Ram Hari Dahal

المصدر: Scientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
Scientific Reports

مصطلحات موضوعية: 0106 biological sciences, 0301 basic medicine, Science, medicine.disease_cause, Microbiology, 010603 evolutionary biology, 01 natural sciences, Genome, Article, 03 medical and health sciences, medicine, Genome size, Gene, Escherichia coli, Multidisciplinary, biology, Phylogenetic tree, Strain (chemistry), Drug discovery, Biological techniques, biology.organism_classification, 16S ribosomal RNA, 030104 developmental biology, Medicine, Bacteria

الوصف: An ivory-coloured, motile, Gram-stain-negative bacterium, designated TW-1T was isolated from oil-contaminated experimental soil in Kyonggi University. The phylogenetic analysis based on 16S rRNA gene sequence revealed, strain TW-1T formed a lineage within the family Oxalobacteraceae and clustered as members of the genus Massilia. The closest members were M. pinisoli T33T (98.8% sequence similarity), M. putida 6NM-7T (98.6%), M. arvi THG-RS2OT (98.5%), M. phosphatilytica 12-OD1T (98.3%) and M. niastensis 5516S-1T (98.2%). The sole respiratory quinone is ubiquinone-8. The major cellular fatty acids are hexadeconic acid, cis-9, methylenehexadeconic acid, summed feature 3 and summed feature 8. The major polar lipids are phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The DNA G + C content of the type strain is 66.3%. The average nucleotide identity (ANI) and in silico DNA–DNA hybridization (dDDH) relatedness values between strain TW-1T and closest members were below the threshold value for species demarcation. The genome size is 7,051,197 bp along with 46 contigs and 5,977 protein-coding genes. The genome showed 5 putative biosynthetic gene clusters (BGCs) that are responsible for different secondary metabolites. Cluster 2 showed thiopeptide BGC with no known cluster blast, indicating TW-1T might produce novel antimicrobial agent. The antimicrobial assessment also showed that strain TW-1T possessed inhibitory activity against Gram-negative pathogens (Escherichia coli and Pseudomonas aeruginosa). This is the first report of the species in the genus Massilia which produces antimicrobial compounds. Based on the polyphasic study, strain TW-1T represents novel species in the genus Massilia, for which the name Massilia antibiotica sp. nov. is proposed. The type strain is TW-1T (= KACC 21627T = NBRC 114363T).

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8bd9ce4b52b8ddd67dcaa244b0e0b9dfTest
https://doi.org/10.1038/s41598-021-86232-zTest

المؤلفون: Dong-Uk Kim, Dhiraj Kumar Chaudhary, Jaisoo Kim, Dockyu Kim

المصدر: Scientific Reports
Scientific Reports, Vol 9, Iss 1, Pp 1-9 (2019)

مصطلحات موضوعية: 0301 basic medicine, lcsh:Medicine, Flavobacterium, Article, 03 medical and health sciences, 0302 clinical medicine, Sequence Homology, Nucleic Acid, Botany, Soil Pollutants, Flavobacterium psychrolimnae, lcsh:Science, Psychrophile, Biotransformation, Phylogeny, Soil Microbiology, Multidisciplinary, biology, Phylogenetic tree, Arctic Regions, Strain (biology), lcsh:R, biology.organism_classification, 16S ribosomal RNA, 030104 developmental biology, Arctic, lcsh:Q, Gasoline, Genome, Bacterial, 030217 neurology & neurosurgery, Bacteria

الوصف: This study presents taxonomic description of two novel diesel-degrading, psychrophilic strains: Kopri-42T and Kopri-43, isolated during screening of oil-degrading psychrotrophs from oil-contaminated Arctic soil. A preliminary 16S rRNA gene sequence and phylogenetic tree analysis indicated that these Arctic strains belonged to the genus Flavobacterium, with the nearest relative being Flavobacterium psychrolimnae LMG 22018T (98.9% sequence similarity). The pairwise 16S rRNA gene sequence identity between strains Kopri-42T and Kopri-43 was 99.7%. The DNA-DNA hybridization value between strain Kopri-42T and Kopri-43 was 88.6 ± 2.1% indicating that Kopri-42T and Kopri-43 represents two strains of the same genomospecies. The average nucleotide identity and in silico DNA-DNA hybridization values between strain Kopri-42T and nearest relative F. psychrolimnae LMG 22018T were 92.4% and 47.9%, respectively. These values support the authenticity of the novel species and confirmed the strain Kopri-42T belonged to the genus Flavobacterium as a new member. The morphological, physiological, biochemical and chemotaxonomic data also distinguished strain Kopri-42T from its closest phylogenetic neighbors. Based on the polyphasic data, strains Kopri-42T and Kopri-43 represents a single novel species of the genus Flavobacterium, for which the name Flavobacterium petrolei sp. nov. is proposed. The type strain is Kopri-42T (=KEMB 9005-710T = KACC 19625T = NBRC 113374T).

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9d2bc4edd3c658dfcf04b05ca6a37e25Test
https://doi.org/10.1038/s41598-019-40667-7Test