Genome insight and description of antibiotic producing Massilia antibiotica sp. nov., isolated from oil-contaminated soil
| العنوان: | Genome insight and description of antibiotic producing Massilia antibiotica sp. nov., isolated from oil-contaminated soil |
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| المؤلفون: | Jaisoo Kim, Dhiraj Kumar Chaudhary, Ram Hari Dahal |
| المصدر: | Scientific Reports, Vol 11, Iss 1, Pp 1-11 (2021) Scientific Reports |
| بيانات النشر: | Springer Science and Business Media LLC, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, Science, medicine.disease_cause, Microbiology, 010603 evolutionary biology, 01 natural sciences, Genome, Article, 03 medical and health sciences, medicine, Genome size, Gene, Escherichia coli, Multidisciplinary, biology, Phylogenetic tree, Strain (chemistry), Drug discovery, Biological techniques, biology.organism_classification, 16S ribosomal RNA, 030104 developmental biology, Medicine, Bacteria |
| الوصف: | An ivory-coloured, motile, Gram-stain-negative bacterium, designated TW-1T was isolated from oil-contaminated experimental soil in Kyonggi University. The phylogenetic analysis based on 16S rRNA gene sequence revealed, strain TW-1T formed a lineage within the family Oxalobacteraceae and clustered as members of the genus Massilia. The closest members were M. pinisoli T33T (98.8% sequence similarity), M. putida 6NM-7T (98.6%), M. arvi THG-RS2OT (98.5%), M. phosphatilytica 12-OD1T (98.3%) and M. niastensis 5516S-1T (98.2%). The sole respiratory quinone is ubiquinone-8. The major cellular fatty acids are hexadeconic acid, cis-9, methylenehexadeconic acid, summed feature 3 and summed feature 8. The major polar lipids are phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The DNA G + C content of the type strain is 66.3%. The average nucleotide identity (ANI) and in silico DNA–DNA hybridization (dDDH) relatedness values between strain TW-1T and closest members were below the threshold value for species demarcation. The genome size is 7,051,197 bp along with 46 contigs and 5,977 protein-coding genes. The genome showed 5 putative biosynthetic gene clusters (BGCs) that are responsible for different secondary metabolites. Cluster 2 showed thiopeptide BGC with no known cluster blast, indicating TW-1T might produce novel antimicrobial agent. The antimicrobial assessment also showed that strain TW-1T possessed inhibitory activity against Gram-negative pathogens (Escherichia coli and Pseudomonas aeruginosa). This is the first report of the species in the genus Massilia which produces antimicrobial compounds. Based on the polyphasic study, strain TW-1T represents novel species in the genus Massilia, for which the name Massilia antibiotica sp. nov. is proposed. The type strain is TW-1T (= KACC 21627T = NBRC 114363T). |
| تدمد: | 2045-2322 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8bd9ce4b52b8ddd67dcaa244b0e0b9dfTest https://doi.org/10.1038/s41598-021-86232-zTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....8bd9ce4b52b8ddd67dcaa244b0e0b9df |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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