Transformation of a partial nopaline synthase gene into tobacco suppresses the expression of a resident wild-type gene
| العنوان: | Transformation of a partial nopaline synthase gene into tobacco suppresses the expression of a resident wild-type gene |
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| المؤلفون: | Daphne R. Goring, Steven J. Rothstein, Lisa Thomson |
| المصدر: | Proceedings of the National Academy of Sciences. 88:1770-1774 |
| بيانات النشر: | Proceedings of the National Academy of Sciences, 1991. |
| سنة النشر: | 1991 |
| مصطلحات موضوعية: | Genetics, Multidisciplinary, Nicotiana tabacum, Wild type, Agrobacterium tumefaciens, Biology, biology.organism_classification, Molecular biology, Transformation (genetics), DNA methylation, Gene expression, Cauliflower mosaic virus, Gene, Research Article |
| الوصف: | A portion of the nopaline synthase gene under the control of the cauliflower mosaic virus 35S promoter was used to transform a tobacco plant that had previously been transformed with a wild-type nopaline synthase (nos) gene. Unexpectedly, in all nine primary transformants tested the wild-type nos expression was virtually completely suppressed. In contrast, plants transformed with the control vector DNA, which differed only in the absence of the partial nos gene, did not show any inhibition of nos expression. Progeny plants were analyzed for the stability of the gene-silencing phenotype. All of the progeny that carried both the wild-type and partial nos genes had no detectable nopaline synthase activity. In addition, wild-type nos mRNA could not be detected in these plants. In most plants in which the wild-type gene was segregated away from the partial nos gene, wild-type levels of activity were detected. Although DNA methylation has been shown to be correlated with a decrease in promoter activity in plants, none of the progeny appeared to carry a methylated nos promoter. The underlying mechanism causing this gene suppression phenomenon is unclear at this time. |
| تدمد: | 1091-6490 0027-8424 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b129474f22d9fe3ef694a7ec991f0252Test https://doi.org/10.1073/pnas.88.5.1770Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....b129474f22d9fe3ef694a7ec991f0252 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10916490 00278424 |
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