التفاصيل البيبلوغرافية
| العنوان: |
A ZTF-7/RPS-2 complex mediates the cold-warm response in C. elegans. |
| المؤلفون: |
Xu, Ting, Liao, Shimiao, Huang, Meng, Zhu, Chengming, Huang, Xiaona, Jin, Qile, Xu, Demin, Fu, Chuanhai, Chen, Xiangyang, Feng, Xuezhu, Guang, Shouhong |
| المصدر: |
PLoS Genetics; 2/10/2023, Vol. 18 Issue 3, p1-21, 21p |
| مصطلحات موضوعية: |
CAENORHABDITIS elegans, HEAT shock proteins, COLD-blooded animals, ZINC-finger proteins, RIBOSOMAL proteins, WARM-blooded animals, BODY temperature |
| مستخلص: |
Temperature greatly affects numerous biological processes in all organisms. How multicellular organisms respond to and are impacted by hypothermic stress remains elusive. Here, we found that cold-warm stimuli induced depletion of the RNA exosome complex in the nucleoli but enriched it in the nucleoplasm. To further understand the function and mechanism of cold-warm stimuli, we conducted forward genetic screening and identified ZTF-7, which is required for RNA exosome depletion from nucleoli upon transient cold-warm exposure in C. elegans. ZTF-7 is a putative ortholog of human ZNF277 that may contribute to language impairments. Immunoprecipitation followed by mass spectrometry (IP-MS) found that ZTF-7 interacted with RPS-2, which is a ribosomal protein of the small subunit and participates in pre-rRNA processing. A partial depletion of RPS-2 and other proteins of the small ribosomal subunit blocked the cold-warm stimuli-induced reduction of exosome subunits from the nucleoli. These results established a novel mechanism by which C. elegans responds to environmental cold-warm exposure. Author summary: Temperature is a very common stimuli for all organisms and widely affects biological processes. Physiological responses to sustain temperature changes constitute a critical adaptive mechanism for living organisms. Warm-blooded animals can maintain a fairly stable body temperature, but cold-blooded animals experience drastic shifts in body temperature. Many studies are focused on heat shock response, but how organisms respond to cold shock stimuli is largely unclear. Here, we observed that the RNA exosome, a highly conserved RNA processing machinery, responds to the hypothermic stress by altering its subcellular localization in C. elegans. The RNA exosome complex localized in nucleoli when the worms were grown at 20°C. A cold-warm treatment at 4°C for 3 hours followed by recovery at 20°C could deplete the RNA exosome from the nucleoli to nucleoplasm. A conserved zinc finger protein ZTF-7 is required for the cold-warm shock-induced exosome translocation. ZTF-7 is an ortholog of human ZNF277 that is likely involved in language impairment. ZTF-7 interacts with a small ribosomal subunit protein RPS-2. Additionally, other small ribosomal subunit proteins, for example RPS-0 and RPS-12, were also involved in the cold-warm shock-induced exosome translocation. We concluded that the ZTF-7/RPS-2 complex mediates a new cold-warm response pathway in C. elegans. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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