التفاصيل البيبلوغرافية
| العنوان: |
Recognition of dileucine-based sorting signals from HIV-1 Nef and LIMP-Il by the AP-1 γ-σ1 and AP-3 Δ-sigma;3 hemicomplexes. |
| المؤلفون: |
Janvier, Katy1, Kato, Yukio1, Boehm, Markus1, Rose, Jeremy R.2, Martina, José A.1, Bong-Yoon Kim, José A.1, Venkatesan, Sundararajan2, Bonifitcino, Juan S.1 juan@helix.nih.gov |
| المصدر: |
Journal of Cell Biology. 12/22/2003, Vol. 163 Issue 6, p1281-1290. 10p. |
| مصطلحات موضوعية: |
*ENDOCYTOSIS, *ABSORPTION (Physiology), *CELL physiology, *PROTEINS, *BIOMOLECULES, *ORGANIC compounds |
| مستخلص: |
The sorting of transmembrane proteins to endosomes and lysosomes is mediated by signals present in the cytosolic tails of the proteins. A subset of these signals conform to the [DE]XXXL[LI] consensus motif and mediate sorting via interactions with heterotetrameric adaptor protein (AP) complexes. However, the identity of the AP subunits that recognize these signals remains controversial. We have used a yeast three-hybrid assay to demonstrate that [DE]XXXL[Ll]-type signals from the human immunodeficiency virus negative factor protein and the lysosomal integral membrane protein II interact with combinations of the γ and σ subunits of AP-1 and the δ and σ3 subunits of AP-3, but not the analogous combinations of AP-2 and AP-4 subunits. The sequence requirements for these interactions are similar to those for binding to the whole AP complexes in vitro and for function of the signals in vivo. These observations reveal a novel mode of recognition of sorting signals involving the γ/δ and σ subunits of AP-1 and AP-3. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
Academic Search Index |
Full Text Finder