دورية
CTCF organizes inter-A compartment interactions through RYBP-dependent phase separation
| العنوان: | CTCF organizes inter-A compartment interactions through RYBP-dependent phase separation |
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| المؤلفون: | Wei, Chao, Jia, Lumeng, Huang, Xiaona, Tan, Jin, Wang, Mulan, Niu, Jing, Hou, Yingping, Sun, Jun, Zeng, Pengguihang, Wang, Jia, Qing, Li, Ma, Lin, Liu, Xinyi, Tang, Xiuxiao, Li, Fenjie, Jiang, Shaoshuai, Liu, Jingxin, Li, Tingting, Fan, Lili, Sun, Yujie, Gao, Juntao, Li, Cheng, Ding, Junjun |
| المصدر: | Cell Research; August 2022, Vol. 32 Issue: 8 p744-760, 17p |
| مستخلص: | Chromatin is spatially organized into three-dimensional structures at different levels including A/B compartments, topologically associating domains and loops. The canonical CTCF-mediated loop extrusion model can explain the formation of loops. However, the organization mechanisms underlying long-range chromatin interactions such as interactions between A–A compartments are still poorly understood. Here we show that different from the canonical loop extrusion model, RYBP-mediated phase separation of CTCF organizes inter-A compartment interactions. Based on this model, we designed and verified an induced CTCF phase separation system in embryonic stem cells (ESCs), which facilitated inter-A compartment interactions, improved self-renewal of ESCs and inhibited their differentiation toward neural progenitor cells. These findings support a novel and non-canonical role of CTCF in organizing long-range chromatin interactions via phase separation. |
| قاعدة البيانات: | Supplemental Index |
Full Text Finder | تدمد: | 10010602 17487838 |
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| DOI: | 10.1038/s41422-022-00676-0 |