دورية أكاديمية
Age-Related Oxidative Changes in Primary Porcine Fibroblasts Expressing Mutated Huntingtin
| العنوان: | Age-Related Oxidative Changes in Primary Porcine Fibroblasts Expressing Mutated Huntingtin |
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| المؤلفون: | Smatlikova, Petra, Askeland, Georgina, Vaskovicova, Michaela, Klima, Jiri, Motlik, Jan, Eide, Lars, Ellederova, Zdenka |
| المصدر: | 1660-2854. |
| بيانات النشر: | S. Karger AG |
| سنة النشر: | 2019 |
| المجموعة: | Universitet i Oslo: Digitale utgivelser ved UiO (DUO) |
| الوصف: | Background: Huntington’s disease (HD) is a devastating neurodegenerative disorder caused by CAG triplet expansions in the huntingtin gene. Oxidative stress is linked to HD pathology, although it is not clear whether this is an effect or a mediator of disease. The transgenic (TgHD) minipig expresses the N-terminal part of human-mutated huntingtin and represents a unique model to investigate therapeutic strategies towards HD. A more detailed characterization of this model is needed to fully utilize its potential. Methods: In this study, we focused on the molecular and cellular features of fibroblasts isolated from TgHD minipigs and the wild-type (WT) siblings at different ages, pre-symptomatic at the age of 24–36 months and with the onset of behavioural symptoms at the age of 48 months. We measured oxidative stress, the expression of oxidative stress-related genes, proliferation capacity along with the expression of cyclin B1 and D1 proteins, cellular permeability, and the integrity of the nuclear DNA (nDNA) and mitochondrial DNA in these cells. Results: TgHD fibroblasts isolated from 48-month-old animals showed increased oxidative stress, which correlated with the overexpression of SOD2 encoding mitochondrial superoxide dismutase 2, and the NEIL3 gene encoding DNA glycosylase involved in replication-associated repair of oxidized DNA. TgHD cells displayed an abnormal proliferation capacity and permeability. We further demonstrated increased nDNA damage in pre-symptomatic TgHD fibroblasts (isolated from animals aged 24–36 months). Conclusions: Our results unravel phenotypic alterations in primary fibroblasts isolated from the TgHD minipig model at the age of 48 months. Importantly, nDNA damage appears to precede these phenotypic alterations. Our results highlight the impact of fibroblasts from TgHD minipigs in studying the molecular mechanisms of HD pathophysiology that gradually occur with age. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | http://urn.nb.no/URN:NBN:no-77978Test; Smatlikova, Petra Askeland, Georgina Vaskovicova, Michaela Klima, Jiri Motlik, Jan Eide, Lars Ellederova, Zdenka . Age-Related Oxidative Changes in Primary Porcine Fibroblasts Expressing Mutated Huntingtin. Neurodegenerative Diseases. 2019, 19(1), 22-34; http://hdl.handle.net/10852/74875Test; 1716190; info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Neurodegenerative Diseases&rft.volume=19&rft.spage=22&rft.date=2019; Neurodegenerative Diseases; 19; 22; 34; https://doi.org/10.1159/000500091Test; URN:NBN:no-77978; Fulltext https://www.duo.uio.no/bitstream/handle/10852/74875/2/Postprint-tversion_Smatlikova_Eide_Cristin-post%2B1716190.pdfTest |
| DOI: | 10.1159/000500091 |
| الإتاحة: | https://doi.org/10.1159/000500091Test http://hdl.handle.net/10852/74875Test http://urn.nb.no/URN:NBN:no-77978Test |
| رقم الانضمام: | edsbas.D1CEAA91 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1159/000500091 |
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