التفاصيل البيبلوغرافية
| العنوان: |
Mitochondrial Respiratory Chain Dysfunction in Dorsal Root Ganglia of Streptozotocin-Induced Diabetic Rats and Its Correction by Insulin Treatment. |
| المؤلفون: |
Chowdhury, Subir K. Roy, Zherebitskaya, Elena, Smith, Darrell R., Akude, Eli, Chattopadhyay, Sharmila, Jolivalt, Corinne G., Calcutt, Nigel A., Fernyhough, Paul |
| المصدر: |
Diabetes; Apr2010, Vol. 59 Issue 4, p1082-1091, 10p, 2 Charts, 6 Graphs |
| مصطلحات موضوعية: |
MITOCHONDRIAL pathology, GANGLIA, STREPTOZOTOCIN, PEOPLE with diabetes, INSULIN therapy |
| مستخلص: |
OBJECTIVE--Impairments in mitochondrial physiology may play a role in diabetic sensory neuropathy. We tested the hypothesis that mitochondrial dysfunction in sensory neurons is due to abnormal mitochondrial respiratory function. RESEARCH DESIGN AND METHODS--Rates of oxygen consumption were measured in mitochondria from dorsal root ganglia (DRG) of 12- to- 22-week streptozotocin (STZ)-induced diabetic rats, diabetic rats treated with insulin, and age-matched controls. Activities and expression of components of mitochondrial complexes and reactive oxygen species (ROS) were analyzed. RESULTS--Rates of coupled respiration with pyruvate + malate (P + M) and with ascorbate + TMPD (Asc + TMPD) in DRG were unchanged after 12 weeks of diabetes. By 22 weeks of diabetes, respiration with P + M was significantly decreased by 31-44% and with Asc + TMPD by 29-39% compared with control. Attenuated mitochondrial respiratory activity of STZ-diabetic rats was significantly improved by insulin that did not correct other indices of diabetes. Activities of mitochondrial complexes I and IV and the Krebs cycle enzyme, citrate synthase, were decreased in mitochondria from DRG of 22-week STZ-diabetic rats compared with control. ROS levels in perikarya of DRG neurons were not altered by diabetes, but ROS generation from mitochondria treated with antimycin A was diminished compared with control. Reduced mitochondrial respiratory function was associated with downregulation of expression of mitochondrial proteins. CONCLUSIONS--Mitochondrial dysfunction in sensory neurons from type I diabetic rats is associated with impaired rates of respiratory activity and occurs without a significant rise in perikaryal ROS. Diabetes 59:1082-1091, 2010 [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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