دورية أكاديمية
Simultaneous production of 3-hydroxypropionic acid and 1,3-propanediol from glycerol using resting cells of the lactate dehydrogenase-deficient recombinant Klebsiella pneumoniae overexpressing an aldehyde dehydrogenase
| العنوان: | Simultaneous production of 3-hydroxypropionic acid and 1,3-propanediol from glycerol using resting cells of the lactate dehydrogenase-deficient recombinant Klebsiella pneumoniae overexpressing an aldehyde dehydrogenase |
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| المؤلفون: | Kumar, Vinod, Sankaranarayanan, Mugesh, Durgapal, Meetu, Zhou, Shengfang, Ko, Yeounjoo, Ashok, Somasundar, Sarkar, Ritam, Park, Sunghoon |
| بيانات النشر: | ELSEVIER SCI LTD |
| سنة النشر: | 2017 |
| المجموعة: | ScholarWorks@UNIST (Ulsan National Institute of Science and Technology) |
| مصطلحات موضوعية: | K. pneumoniae J2B KGSADH, K. pneumoniae J2B ??ldhA KGSADH, 3-Hydroxypropionic acid, 1,3-Propanediol, Lactate dehydrogenase |
| الوصف: | In the present study, the lactate dehydrogenase-deficient (ldhA(-)) recombinant Klebsiella pneumoniae overexpressing an ALDH (KGSADH) was developed and the co-production of 3-HP and PDO from glycerol by this recombinant under resting cell conditions was examined. The new recombinant did not produce any appreciable lactate, which seriously inhibits the production of 3-HP and PDO. The final titers of 3-HP and PDO by the ldhA(-) recombinant strain at 60 h were 252.2 mM and 308.7 mM, respectively, which were improved by approximately 30% and 50%, respectively, compared to those by the counterpart recombinant strain, which was the wild type for ldhA. In addition, after deleting ldhA, the cumulative yield on glycerol and specific production rate of these two metabolites (3-HP and PDO) were enhanced by 41.4% and 52%, respectively. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0960-8524 |
| العلاقة: | BIORESOURCE TECHNOLOGY, v.135, no., pp.555 - 563; http://www.sciencedirect.com/science/article/pii/S0960852412016914Test; https://scholarworks.unist.ac.kr/handle/201301/25328Test; 3392; 27927; 2-s2.0-84876490011; 000319181000076 |
| DOI: | 10.1016/j.biortech.2012.11.018 |
| الإتاحة: | https://doi.org/10.1016/j.biortech.2012.11.018Test https://scholarworks.unist.ac.kr/handle/201301/25328Test http://www.sciencedirect.com/science/article/pii/S0960852412016914Test |
| رقم الانضمام: | edsbas.E1808BE |
| قاعدة البيانات: | BASE |
| تدمد: | 09608524 |
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| DOI: | 10.1016/j.biortech.2012.11.018 |