دورية أكاديمية
Aktivnosti izoenzima laktat dehidrogenaze i učinci na slezenu štakora tretiranih tomilom ; Increase in Lactate Dehydrogenase Isoenzyme-4 and Splenocyte Toxicity in Methomyl-Treated Rats
| العنوان: | Aktivnosti izoenzima laktat dehidrogenaze i učinci na slezenu štakora tretiranih tomilom ; Increase in Lactate Dehydrogenase Isoenzyme-4 and Splenocyte Toxicity in Methomyl-Treated Rats |
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| المؤلفون: | Lohitnavy, Ornrat, Sinhaseni, Palarp |
| المصدر: | Archives of Industrial Hygiene and Toxicology (arhiv@imi.hr); Vol.49 No.3 |
| بيانات النشر: | Institute for Medical Research and Occupational Health |
| سنة النشر: | 1999 |
| المجموعة: | Hrčak - Portal of scientific journals of Croatia / Portal znanstvenih časopisa Republike Hrvatske |
| مصطلحات موضوعية: | insekticidi, karbamati, kolinesteraza, laktat dehidrogenaze, metomil, slezena, štakori, carbamates, cholinesterases, insecticides, lactate dehydrogenase, methomyl, rats, spelnocyte, splenotoxicity |
| الوصف: | Istraživani su učinci karbamatnog insekticida metomila u štakora. Nakon jednokratne peroralne doze od 3, 5 ili 7 mg/kg nađeno je značajno povećanje sveukupne aktivnosti laktat dehidrogenaze (LDH) nakon prvog dana tretmana. Najveća je aktivnost utvrđena trećeg dana nakon tretmana metomilom u dozi od 7 mg/kg. Enzimska aktivnost postepeno se smanjivala i sedmoga se dana normalizirala. Posebno je zapaženo povećanje aktivnosti izoenzima LDH-3 i LDH-4. U posebnim je pokusima utvrđeno značajno smanjenje težine slezene i vijabilnosti splenocita u štakora tretiranih sa 6 mg/kg i 8 mg/kg metomila i to nakon prvog i nakon trećeg dana tretmana. Toksični učinci na slezenu mogli su se spriječiti pretretiranjem sa 60 mg/kg N-acetilcisteina. Prema rezultatima ovih pokusa čini se da bi toksični učinak metomila na slezenu mogao biti izravan učinak metomila na stanice slezene a ne putem kolinergičnog mehanizma. Važnost ovakvog citotoksičnog učinka i mehanizme citotoksičnosti i njihove povezanosti s reaktivnim oksidativnim procesima pri staničnom oštećenju valja još istraživati. ; The toxic effect of methomyl was studied in rats after a single or repeated oral administration. Rats treated with a single dose of methomyl (3, 5, or 7 mg/kg) showed significant increase (P<0.05) in total lactate dehydrogenase activity on day 1. The highest level of LDH activity was observed on day 3 in rats receiving 7 mg/kg of methomyl. The total LDH activity returned to normal on day 7 after dosing. Specific increases in LDH-3 and LDH-4 isoenzyme activities were observed. In rats treated with a single dose of 6 and 8 mg/kg of methomyl, spleen weight and splenocyte viability significantly dropped (P<0.05) on days 1 and 3, respectively. Splenotoxicity was prevented by pretreatment with 60 mg/kg of N-acetylcysteine. The results suggest that the splenotoxic effect of methomyl is more likely directly related to oxidative cell injury than to cholinesterase inhibition. The significance of cytotoxic effects and the nature of cytotoxicity in relation to ... |
| نوع الوثيقة: | text |
| وصف الملف: | |
| اللغة: | English |
| العلاقة: | http://hrcak.srce.hr/3280Test; http://hrcak.srce.hr/file/5519Test |
| الإتاحة: | http://hrcak.srce.hr/3280Test http://hrcak.srce.hr/file/5519Test |
| حقوق: | Copyright: Institute for Medical Research and Occupational Health, Zagreb, Croatia Parts of the contents such as figures and tables may be reproduced without prior permission, provided reference is made to the source. Annual subscription to the printed version (postage included): Europe Eur 70.00; overseas countries Eur 85,00 Slovenian Society of Toxicology Eur 30.00 Full text articles online available for free. |
| رقم الانضمام: | edsbas.69379DC7 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |