التفاصيل البيبلوغرافية
| العنوان: |
A Comparative Transcriptome Analysis of Avocado Embryogenic Lines Susceptible or Resistant to Rosellinia necatrix Exudate. |
| المؤلفون: |
Moreno-Pérez, Ana, Zumaquero, Adela, Martínez-Ferri, Elsa, López-Herrera, Carlos, Pliego-Alfaro, Fernando, Palomo-Ríos, Elena, Pliego, Clara |
| المصدر: |
Agronomy; May2023, Vol. 13 Issue 5, p1354, 18p |
| مصطلحات موضوعية: |
AVOCADO, ROOT rots, EXUDATES & transudates, LIGNINS, MYCOSES, PROTEINASES, RNA sequencing, TRANSCRIPTION factors |
| مستخلص: |
Avocado embryogenic cultures were selected for resistance to the culture filtrate (CF) of Rosellinia necatrix, the causal agent of White Root Rot disease. A resistant callus line was obtained through recurrent selections in progressively increasing concentrations of fungal CF (from 60% to 80%). RNA sequencing (RNA-Seq) technology was used to compare the transcriptomic profiles of the avocado embryogenic-callus-resistant line L3 (capable to survive in the presence of 80% CF) and control line AN-9 (not exposed to CF), after 24 h of growth in a medium containing 40% CF. A total of 25,211 transcripts were obtained, of which 4,918 and 5,716 were differentially expressed in the resistant and control line, respectively. Interestingly, exposure of embryogenic callus lines to 40% of R. necatrix exudates induced genes previously reported to be related to avocado defense against fungal diseases (lignin biosynthesis, Pathogenesis Related (PR) proteins, WRKY (WRKYGQK) Transcription Factor (TF), NAC (NAM, ATAF1/2, and CUC2) TF, proteinase inhibitors and Ethylene Response Transcription Factor (ERF), among others), which were accumulated in greater amounts in the resistant line in comparison to the susceptible one. This research will contribute to the understanding of avocado defense against this pathogen, thereby aiding in the selection of resistant avocado rootstocks. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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