Adipocyte ADRB3 Down-Regulated in Chinese Overweight Individuals Adipocyte ADRB3 in Overweight
| العنوان: | Adipocyte ADRB3 Down-Regulated in Chinese Overweight Individuals Adipocyte ADRB3 in Overweight |
|---|---|
| المؤلفون: | Wen-Yue Cao, Xiao Yin, Jing Yu, Zhao Liu, Han Li, Feng Guo |
| المصدر: | Obesity Facts, Vol 11, Iss 6, Pp 524-533 (2018) |
| بيانات النشر: | S. Karger AG, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Health (social science), Adipose tissue, Overweight, chemistry.chemical_compound, Adipocyte, Brown adipose tissue, Adipocytes, β3-adrenoceptor, lcsh:RC620-627, Cells, Cultured, Uncoupling Protein 1, Middle Aged, lcsh:Nutritional diseases. Deficiency diseases, medicine.anatomical_structure, Adipose Tissue, Female, medicine.symptom, lcsh:Nutrition. Foods and food supply, Research Article, Human, Adult, medicine.medical_specialty, Stromal cell, Primary Cell Culture, Down-Regulation, lcsh:TX341-641, Young Adult, 03 medical and health sciences, Asian People, Physiology (medical), Internal medicine, medicine, Humans, Obesity, Inverse correlation, Aged, Messenger RNA, business.industry, medicine.disease, 030104 developmental biology, Endocrinology, chemistry, Receptors, Adrenergic, beta-3, Insulin Resistance, business |
| الوصف: | Objectives: Activation of β3-adrenoceptor (ADRB3) is essential in the process of human adipose tissue browning, but obese subjects suffered from reduced ability of brown adipose tissue activation. The present study aims to detect the adipocyte ADRB3 expression in overweight individuals and the relationship between adipocyte ADRB3 expression and adiposity in adults. Methods: Visceral adipose tissue samples were obtained from 85 subjects who underwent abdominal surgery. ADRB3 mRNA and protein expression levels in mature adipocytes and adipose tissue stromal vascular cells were examined by quantitative real-time PCR and Western blot assay, respectively. UCP-1mRNA expression levels in mature adipocytes were examined by quantitative real-time PCR. Results: The data revealed that ADRB3 mRNA (p = 0.021) and protein (p = 0.025) expression levels in mature adipocytes were significantly higher in the normal-weight than in the overweight group. Similar results were also found for ADRB3 mRNA (p = 0.041) and protein (p = 0.025) expressions of stromal vascular cells. An inverse correlation was verified between mature adipocyte ADRB3 mRNA expression and BMI (r = –0.362, p = 0.012). UCP-1 mRNA expression levels in mature adipocytes were higher in the normal-weight group compared with the overweight group (p = 0.045). Conclusion: Adipocyte ADRB3 expression levels were down-regulated before the onset of obesity, which indicated that the reduction of ADRB3 expression might be the cause of compromised adipose tissue browning and obesity rather than the result. Thus, the interference of the ADRB3 pathway in adipocytes may provide a potential treatment target for obesity. |
| تدمد: | 1662-4033 1662-4025 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7839a912db34b8d13b1375ffbe15dcb9Test https://doi.org/10.1159/000495116Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....7839a912db34b8d13b1375ffbe15dcb9 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16624033 16624025 |
|---|