المؤلفون: Farrag, Mariam, Ait Eldjoudi, Djedjiga, González-Rodríguez, María, Cordero-Barreal, Alfonso, Ruiz-Fernández, Clara, Capuozzo, Maurizio, González-Gay Mantecón, Miguel Ángel, Mera, Antonio, Lago, Francisca, Soffar, Ahmed, Essawy, Amina, Pino, Jesus, Farrag, Yousof, Gualillo, Oreste

المساهمون: Universidad de Cantabria

المصدر: Frontiers in endocrinology, 2023, 13, 1101091

مصطلحات موضوعية: Asprosin, Adipokines, Metabolic diseases, Obesity, Diabetes, PCOS

الوصف: Adipose tissue malfunction leads to altered adipokine secretion which might consequently contribute to an array of metabolic diseases spectrum including obesity, diabetes mellitus, and cardiovascular disorders. Asprosin is a novel diabetogenic adipokine classified as a caudamin hormone protein. This adipokine is released from white adipose tissue during fasting and elicits glucogenic and orexigenic effects. Although white adipose tissue is the dominant source for this multitask adipokine, other tissues also may produce asprosin such as salivary glands, pancreatic B-cells, and cartilage. Significantly, plasma asprosin levels link to glucose metabolism, lipid profile, insulin resistance (IR), and b-cell function. Indeed, asprosin exhibits a potent role in the metabolic process, induces hepatic glucose production, and influences appetite behavior. Clinical and preclinical research showed dysregulated levels of circulating asprosin in several metabolic diseases including obesity, type 2 diabetes mellitus (T2DM), polycystic ovarian syndrome (PCOS), non-alcoholic fatty liver (NAFLD), and several types of cancer. This review provides a comprehensive overview of the asprosin role in the etiology and pathophysiological manifestations of these conditions. Asprosin could be a promising candidate for both novel pharmacological treatment strategies and diagnostic tools, although developing a better understanding of its function and signaling pathways is still needed. ; Funding: OG and FL are Staff Personnel (I3SNS Stable Researcher) of Xunta de Galicia (Servizo Galego de Saude (SERGAS)) through a research-staff contract (ISCIII)/SERGAS). OG and MG-G are members of RICORS Program, RD21/0002/0025 via ISCIII and FEDER. FL is a member of Centro de Investigación Biomédica en Red de Enfermedades Cardiovasculares (CIBERCV). The work of OG and JP (PI20/00902), and FL (PI21/01145 and CB16/11/00226) is funded by ISCIII and FEDER. OG is a beneficiary of a project funded by the Research Executive Agency of the European Union in the ...

العلاقة: https://hdl.handle.net/10902/29839Test

الإتاحة: https://doi.org/10.3389/fendo.2022.1101091Test
https://hdl.handle.net/10902/29839Test

المؤلفون: Schol, Jordy, Ambrosio, Luca, Tamagawa, Shota, Joyce, Kieran, Ruiz-Fernández, Clara, Nomura, Akira, Sakai, Daisuke

المصدر: Scientific Reports; 6/4/2024, Vol. 14 Issue 1, p1-16, 16p

مصطلحات موضوعية: HERNIA, LACTATE dehydrogenase, WEB databases, PAIN measurement, SCIENCE databases

مستخلص: Lumbar disc herniation (LDH) is often managed surgically. Enzymatic chemonucleolysis emerged as a non-surgical alternative. This systematic review and meta-analysis aims to assess the efficacy and safety of chemonucleolytic enzymes for LDH. The primary objective is to evaluate efficacy through "treatment success" (i.e., pain reduction) and severe adverse events (SAEs) rates. Additionally, differences in efficacy and safety trends among chemonucleolytic enzymes are explored. Following our PROSPERO registered protocol (CRD42023451546) and PRISMA guidelines, a systematic search of PubMed and Web of Science databases was conducted up to July 18, 2023. Inclusion criteria involved human LDH treatment with enzymatic chemonucleolysis reagents, assessing pain alleviation, imaging changes, and reporting on SAEs, with focus on allergic reactions. Quality assessment employed the Cochrane Source of Bias and MINORS tools. Meta-analysis utilized odds ratios (OR) with 95% confidence intervals (CI). Among 62 included studies (12,368 patients), chemonucleolysis demonstrated an 79% treatment success rate and significantly outperformed placebo controls (OR 3.35, 95% CI 2.41–4.65) and scored similar to surgical interventions (OR 0.65, 95% CI 0.20–2.10). SAEs occurred in 1.4% of cases, with slightly higher rates in chymopapain cohorts. No significant differences in "proceeding to surgery" rates were observed between chemonucleolysis and control cohorts. Limitations include dated and heterogeneous studies, emphasizing the need for higher-quality trials. Further optimization through careful patient selection and advances in therapy implementation may further enhance outcomes. The observed benefits call for wider clinical exploration and adoption. No funding was received for this review. [ABSTRACT FROM AUTHOR]

: Copyright of Scientific Reports is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Ambrosio, Luca, Schol, Jordy, Ruiz-Fernandez, Clara, Tamagawa, Shota, Soma, Hazuki, Tilotta, Veronica, Di Giacomo, Giuseppina, Cicione, Claudia, Nakayama, Shunya, Kamiya, Kosuke, Papalia, Rocco, Sato, Masato, Vadalà, Gianluca, Watanabe, Masahiko, Denaro, Vincenzo, Sakai, Daisuke

المصدر: Eur Spine J ; ISSN:1432-0932 ; Volume:33 ; Issue:5

مصطلحات موضوعية: Disc degeneration, Exosomes, Extracellular vesicles, In vivo, Intervertebral disc, Tie2

الوصف: To investigate the therapeutic potential of extracellular vesicles (EVs) derived from human nucleus pulposus cells (NPCs), with a specific emphasis on Tie2-enhanced NPCs, compared to EVs derived from human bone marrow-derived mesenchymal stromal cells (BM-MSCs) in a coccygeal intervertebral disc degeneration (IDD) rat model.

العلاقة: https://doi.org/10.1007/s00586-024-08163-3Test; https://pubmed.ncbi.nlm.nih.gov/38416190Test

الإتاحة: https://doi.org/10.1007/s00586-024-08163-3Test
https://pubmed.ncbi.nlm.nih.gov/38416190Test

المؤلفون: Ait Eldjoudi, Djedjiga, Cordero Barreal, Alfonso, González-Rodriguez, María, Ruiz-Fernández, Clara, Farrag, Yousof, Lago, Francisca, Capuozzo, Maurizio, González-Gay Mantecón, Miguel Ángel, Mera Varela, Antonio, Pino, Jesús, Gualillo, Oreste

المساهمون: Universidad de Cantabria

المصدر: Int J Mol Sci. 2022, 23(5), 2859

مصطلحات موضوعية: Leptin, Leptin receptor, Rheumatoid arthritis, Osteoarthritis, Inflammation

الوصف: White adipose tissue (WAT) is a specialized tissue whose main function is lipid synthesis and triglyceride storage. It is now considered as an active organ secreting a plethora of hormones and cytokines namely adipokines. Discovered in 1994, leptin has emerged as a key molecule with pleiotropic functions. It is primarily recognized for its role in regulating energy homeostasis and food intake. Currently, further evidence suggests its potent role in reproduction, glucose metabolism, hematopoiesis, and interaction with the immune system. It is implicated in both innate and adaptive immunity, and it is reported to contribute, with other adipokines, in the cross-talking networks involved in the pathogenesis of chronic inflammation and immune-related diseases of the musculoskeletal system such as osteoarthritis (OA) and rheumatoid arthritis (RA). In this review, we summarize the most recent findings concerning the involvement of leptin in immunity and inflammatory responses in OA and RA. ; O.G. and F.L. are staff personnel (I3SNS stable researchers) of Xunta de Galicia (Servizo Galego de Saude, SERGAS) through a research-staff contract (ISCIII/SERGAS). C.R.F. is a predoctoral research scholar funded by ISCIII and FEDER (Exp.18/00188). M.G.R. is a recipient of a pre-doctoral contract funded by Xunta de Galicia (IN606A-2020/010). A.C.B. is a recipient of a pre-doctoral contract funded by Secretaría de Estado de Universidades, Investigación, Desarrollo e Innovación, Ministerio de Universidades (FPU2018-04165). O.G. and MAGG are members of the RICORS Programme, RD21/0002/0025 via ISCIII and FEDER. F.L. is a member of Centro de Investigación Biomédica en Red de Enfermedades Cardiovasculares (CIBERCV). The work of O.G. and J.P. (PI17/00409 and PI20/00902), and F.L. (PI18/00821, PI21/01145) is funded by ISCIII and FEDER. O.G. is a beneficiary of a project funded by the Research Executive Agency of the European Union in the framework of MSCA-RISE Action of the H2020 Programme (project number 734899). O.G. is beneficiary of a ...

العلاقة: https://doi.org/10.3390Test/ ijms23052859; https://hdl.handle.net/10902/28633Test

الإتاحة: https://doi.org/10.3390/ijms23052859Test
https://hdl.handle.net/10902/28633Test

المؤلفون: González-Rodríguez, María, Edjoudi, Djedjiga Ait, Cordero Barreal, Alfonso, Ruiz-Fernández, Clara, Farrag, Mariam, González-Rodríguez, Beatriz, Lago, Francisca, Capuozzo, Maurizio, González-Gay Mantecón, Miguel Ángel, Mera Varela, Antonio, Pino, Jesús, Farrag, Yousof, Oreste, Gualillo

المساهمون: Universidad de Cantabria

المصدر: Pharmaceuticals 2022, 15, 1544

مصطلحات موضوعية: Progranulin, Inflammatory diseases, Rheumatoid arthritis, Lupus, Intervertebral disc disease

الوصف: Progranulin (PGRN) is a glycoprotein formed by 593 amino acids encoded by the GRN gene. It has an important role in immunity and inflammatory response, as well as in tissue recovery. Its role in musculoskeletal inflammatory diseases such as rheumatoid arthritis (RA), systemic lupus erythematosus (SLE) and intervertebral disc degeneration disease (IVDD), is, nowadays, an important target to investigate. The objective of this review is to systematically sum up all the recent findings concerning PGRN as a target in the development and resolution of the inflammatory diseases. PubMed was examined with the terms combinations (Progranulin) AND (Lupus Erythematosus, Systemic), (Progranulin) AND (Arthritis, Rheumatoid), and (Progranulin) AND (Intervertebral Disc Degeneration). PubMed was examined with the terms combinations (Atsttrin) AND (Lupus Erythematosus, Systemic), (Atsttrin) AND (Arthritis, Rheumatoid), and (Atsttrin) AND (Intervertebral Disc Degeneration). Moreover, research through Web of Science was performed searching the same items. The inclusion criteria were: studies whose main topic were progranulin, or atsttrin, with emphasis on the three selected diseases. On the other hand, the exclusion criteria were studies that only focused on diseases not related to RA, lupus or IVDD, in addition to the previous published literature reviews. Since few results were obtained, we did not filter by year. The records assessed for eligibility were 23, including all the studies with the information in state of art of progranulin and its capability to be a potential target or treatment for each one of the selected diseases. As these results are descriptive and not clinical trials, we did not perform risk of bias methods. Within these results, many studies have shown an anti-inflammatory activity of PGRN in RA. PGRN levels in serum and synovial fluids in RA patients were reported higher than controls. On the other hand, serum levels were directly correlated with SLE disease activity index, suggesting an important role of ...

العلاقة: https://hdl.handle.net/10902/28346Test

الإتاحة: https://doi.org/10.3390/ph15121544Test
https://hdl.handle.net/10902/28346Test

المؤلفون: Ruiz-Fernández, Clara, González-Rodríguez, María, Abella, Vanessa, Francisco, Vera, Cordero-Barreal, Alfonso, Ait Eldjoudi, Djedjiga, Farrag, Yousof, Pino, Jesús, Conde-Aranda, Javier, González-Gay, Miguel Ángel, Mera, Antonio, Mobasheri, Ali, García-Caballero, Lucía, Gándara-Cortés, Marina, Lago, Francisca, Scotece, Morena, Gualillo, Oreste

المساهمون: Ministerio de Educación, Cultura y Deporte, Lietuvos Mokslo Taryba, Ministry of Economy and Competitiveness | Instituto de Salud Carlos III, Consellería de Economía, Emprego e Industria, Xunta de Galicia

المصدر: Laboratory Investigation ; volume 102, issue 9, page 989-999 ; ISSN 0023-6837

مصطلحات موضوعية: Cell Biology, Molecular Biology, Pathology and Forensic Medicine

الإتاحة: https://doi.org/10.1038/s41374-022-00793-9Test
https://www.nature.com/articles/s41374-022-00793-9.pdfTest
https://www.nature.com/articles/s41374-022-00793-9Test
https://api.elsevier.com/content/article/PII:S0023683722002756?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0023683722002756?httpAccept=text/plainTest

المؤلفون: Farrag, Yousof, Ait Eldjoudi, Djedjiga, Farrag, Mariam, González-Rodríguez, María, Ruiz-Fernández, Clara, Cordero, Alfonso, Varela-García, María, Torrijos Pulpón, Carlos, Bouza, Rebeca, Lago, Francisca, Pino, Jesus, Alvarez-Lorenzo, Carmen, Gualillo, Oreste

المصدر: Polymers (20734360); Dec2023, Vol. 15 Issue 24, p4635, 16p

مصطلحات موضوعية: HYDROGELS, ETHYLENE glycol, METHACRYLATES, METHYL ether, NUCLEAR magnetic resonance spectroscopy, METHYL methacrylate, NITRIC-oxide synthases

مستخلص: Here, we present the synthesis of a series of chemical homopolymeric and copolymeric injectable hydrogels based on polyethylene glycol methyl ether methacrylate (PEGMEM) alone or with 2-dimethylamino ethyl methacrylate (DMAEM). The objective of this study was to investigate how the modification of hydrogel components influences the swelling, rheological attributes, and in vitro biocompatibility of the hydrogels. The hydrogels' networks were formed via free radical polymerization, as assured by ¹H nuclear magnetic resonance spectroscopy (¹H NMR). The swelling of the hydrogels directly correlated with the monomer and the catalyst amounts, in addition to the molecular weight of the monomer. Rheological analysis revealed that most of the synthesized hydrogels had viscoelastic and shear-thinning properties. The storage modulus and the viscosity increased by increasing the monomer and the crosslinker fraction but decreased by increasing the catalyst. MTT analysis showed no potential toxicity of the homopolymeric hydrogels, whereas the copolymeric hydrogels were toxic only at high DMEAM concentrations. The crosslinker polyethylene glycol dimethacrylate (PEGDMA) induced inflammation in ATDC5 cells, as detected by the significant increase in nitric oxide synthase type II activity. The results suggest a range of highly tunable homopolymeric and copolymeric hydrogels as candidates for cartilage regeneration. [ABSTRACT FROM AUTHOR]

: Copyright of Polymers (20734360) is the property of MDPI and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: González-Rodríguez, María, Ait Edjoudi, Djedjiga, Cordero-Barreal, Alfonso, Farrag, Mariam, Varela-García, María, Torrijos-Pulpón, Carlos, Ruiz-Fernández, Clara, Capuozzo, Maurizio, Ottaiano, Alessandro, Lago, Francisca, Pino, Jesús, Farrag, Yousof, Gualillo, Oreste

المصدر: Antioxidants; Dec2023, Vol. 12 Issue 12, p2112, 16p

مصطلحات موضوعية: OLIVE oil, PHENOLS, MEDITERRANEAN diet, RHEUMATISM, INFLAMMATION

مستخلص: Background: The Mediterranean diet is linked to various health benefits, especially the consumption of olive oil as a key component. Multiple studies highlight its advantages, particularly due to its fatty acid composition and additional components like phenolic compounds. A significant antioxidant compound, oleocanthal, known for its antioxidant properties, has gained attention in the pharmaceutical industry for its anti-inflammatory and antiproliferative effects. It shows promise in addressing cardiovascular diseases, metabolic syndrome, and neuroprotection. This systematic review aims to evaluate the existing literature on oleocanthal, examining its role in biological processes and potential impact on conditions like inflammation and cancer. Methods: We performed several searches in PubMed (MEDLINE), Web of Science (WOS), and Cochrane based on the terms "Oleocanthal", "Cancer", and "Inflammation". The inclusion criteria were as follows: studies whose main topics were oleocanthal and cancer or inflammation. On the other hand, the exclusion criteria were studies that were not focused on oleocanthal, reviews, or editorial material. Given that these findings are explanatory rather than derived from clinical trials, we refrained from employing methods to assess potential bias. This systematic review did not receive any external funding. Results: We found 174 records from these searches, where we discarded reviews and editorial material, duplicated articles, and 1 retracted article. Finally, we had 53 reports assessed for eligibility that were included in this review. Discussion: OC exhibits promising therapeutic potential against both inflammation and cancer. We addressed its ability to target inflammatory genes and pathways, offering potential treatments for conditions like rheumatic diseases by regulating pathways such as NF-kB and MAPK. Additionally, OC's anticancer properties, particularly its notable inhibition of c-Met signaling across various cancers, highlight its efficacy, showcasing promise as a potential treatment. [ABSTRACT FROM AUTHOR]

: Copyright of Antioxidants is the property of MDPI and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Conde, Javier, Ruiz-Fernandez, Clara, Francisco, Vera, Scotece, Morena, Gómez, Rodolfo, Lago, Francisca, González-Gay Mantecón, Miguel Ángel, Pino, Jesús, Mobasheri, Ali, Gualillo, Oreste

المساهمون: Universidad de Cantabria

المصدر: Cartilage . 2021 Dec;13(2_suppl):925S-934S

مصطلحات موضوعية: Chondrocyte, Inflammation, Metalloprotease

الوصف: Objective: Osteoarthritis (OA) is an age-related biomechanical and low-grade inflammometabolic disease of the joints and one of the costliest and disabling forms of arthritis. Studies on matrix-degrading enzymes such as metalloproteases, which are implicated in the increased catabolism of extracellular matrix, are of paramount relevance. DKK3 is a member of DKK family and is best known for its role in cancer. Although there is some information about the participation of DKK3 in cartilage pathophysiology and on metalloproteases regulation, in particular, little is known about DKK3 signaling mechanisms. Thus, the aim of this study is to explore how DKK3 regulates matrix metalloproteinase-13 (MMP-13) expression. Design: Gene, protein expression and protein phosphorylation in primary human chondrocytes and ATDC5 mouse cells were assessed by RT-qPCR and Western blot analysis. Further studies on DKK3 activity were performed by targeting DKK3 gene with a specific siRNA. Results: DKK3 expression was found to be higher in OA human chondrocytes than healthy cells, being its expression decreased in interleukin-1? (IL-1?)-stimulated cells. DKK3 knockdown increased the induction of MMP-13 elicited by IL-1? in human and mouse chondrocytes and after the analysis of different signalling pathways, we observed that NF-?B pathway was involved in the regulation of MMP-13 expression by DKK3. Conclusions: Herein we have demonstrated, for the first time, that DKK3 gene silencing exacerbated NF-?B activation, resulting in an increased IL-1?-driven induction of MMP-13. Our results further confirm that DKK3 may play a protective role in OA by attenuating NF-?B activation and the subsequent production of metalloproteases. ; Funding: OG and FL are Staff Personnel of Xunta de Galicia (Servizo Galego de Saude, SERGAS) through a research-staff stabilization contract (ISCIII/SERGAS). JC is “Miguel Servet” Researcher “CP19/00172 (ISCIII/FEDER), MS and VF are currently “Sara Borrell” Researchers funded by ISCIII and FEDER (CD16/00111). RG is a ...

العلاقة: https://doi.org/10.1177/1947603520933328Test; http://hdl.handle.net/10902/24455Test

الإتاحة: https://doi.org/10.1177/1947603520933328Test
http://hdl.handle.net/10902/24455Test

المؤلفون: Cordero-Barreal, Alfonso, González-Rodríguez, María, Ruiz-Fernández, Clara, Eldjoudi, Djedjiga Ait, AbdElHafez, Yousof Ramadan Farrag, Lago, Francisca, Conde, Javier, Gómez, Rodolfo, González-Gay Mantecón, Miguel Ángel, Mobasheri, Ali, Pino, Jesus, Gualillo, Oreste

المساهمون: Universidad de Cantabria

المصدر: Int J Mol Sci . 2021 Feb 27;22(5):2411

مصطلحات موضوعية: Leptin, Articular cartilage, Chondrocyte, LEPR (ObR), Osteoarthritis (OA), Rheumatoid arthritis (RA)

الوصف: Since its discovery in 1994, leptin has been considered as an adipokine with pleiotropic effects. In this review, we summarize the actual information about the impact of this hormone on cartilage metabolism and pathology. Leptin signalling depends on the interaction with leptin receptor LEPR, being the long isoform of the receptor (LEPRb) the one with more efficient intracellular signalling. Chondrocytes express the long isoform of the leptin receptor and in these cells, leptin signalling, alone or in combination with other molecules, induces the expression of pro-inflammatory molecules and cartilage degenerative enzymes. Leptin has been shown to increase the proliferation and activation of immune cells, increasing the severity of immune degenerative cartilage diseases. Leptin expression in serum and synovial fluid are related to degenerative diseases such as osteoarthritis (OA), rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). Inhibition of leptin signalling showed to have protective effects in these diseases showing the key role of leptin in cartilage degeneration. ; Acknowledgments: This work was supported by Xunta de Galicia (Servizo Galego de Saude, SERGAS), through a research-staff contract (ISCIII/SERGAS) to OG and FL, which are Staff Personnel (I3SNS stable Researcher), by Instituto de Salud Carlos III (ISCIII) and by FEDER through a predoctoral research scholar to CR-F (Exp.18/00188), and “Miguel Servet” Researcher contract to RG and JC. MG is a recipient of pre-doctoral contract funded by Xunta de Galicia (IN606A-2020/010). AC is a recipient of a pre-doctoral contract funded by Secretaría de Estado de Universidades, Investigación, Desarrollo e Innovación, Ministerio de Universidades (FPU2018-04165). OG is member of RETICS Programme, [RD16/0012/0014] (RIER: Red de Investigación en Inflamación y Enfermedades Reumáticas) via ISCIII and FEDER. FL is a member of CIBERCV (Centro de Investigación Biomédica en Red de Enfermedades Cardiovasculares). ISCIII and FEDER also support OG and JP ...

العلاقة: https://doi.org/10.3390Test/ ijms22052411; http://hdl.handle.net/10902/24454Test

الإتاحة: https://doi.org/10.3390/ijms22052411Test
http://hdl.handle.net/10902/24454Test