دورية أكاديمية
Platelet-Activating Factor Antagonists Decrease Follicular Dendritic-Cell Stimulation of Human B Lymphocytes
| العنوان: | Platelet-Activating Factor Antagonists Decrease Follicular Dendritic-Cell Stimulation of Human B Lymphocytes |
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| المؤلفون: | Halickman Isaac, Bastien Yolande, Zhuang Qianli, Mazer Monty B, Toledano Baruch, Mazer Bruce D |
| المصدر: | Allergy, Asthma & Clinical Immunology, Vol 1, Iss 2, Pp 49-57 (2005) |
| بيانات النشر: | BMC, 2005. |
| سنة النشر: | 2005 |
| المجموعة: | LCC:Immunologic diseases. Allergy |
| مصطلحات موضوعية: | Immunologic diseases. Allergy, RC581-607 |
| الوصف: | Abstract Both B-lymphoblastoid cell lines and tonsillar B lymphocytes express receptors for platelet-activating factor (PAF). In lymph node germinal centres, B lymphocytes interact with follicular dendritic cells (FDCs), which present antigen-containing immune complexes to B lymphocytes. FDCs have phenotypic features that are similar to those of stromal cells and monocytes and may therefore be a source of lipid mediators. In this study, we evaluated the effects of the PAF antagonist WEB 2170 on the activation of tonsillar B lymphocytes by FDCs. FDCs were isolated from tonsils by Bovine Serum Albumin (BSA) gradient centrifugation. After being cultured for 6 to 10 days, they were incubated with freshly isolated B cells in the presence or absence of the specific PAF receptor antagonist WEB 2170. B-lymphocyte proliferation was assessed by [3H]-thymidine incorporation, and immunoglobulin (Ig) G and IgM secretion was assessed by enzyme-linked immunosorbent assay (ELISA). WEB 2170 (10-6 to 10-8 M) inhibited [3H]-thymidine incorporation by up to 35% ± 3%. Moreover, the secretion of IgG and IgM was inhibited by up to 50% by WEB 2170 concentrations ranging from 10-6 to 10-8 M. There was no evidence of toxicity by trypan blue staining, and the addition of WEB 2170 to B cells in the absence of FDCs did not inhibit the spontaneous production of IgG or IgM. The effect of the PAF antagonist is primarily on B lymphocytes, as reverse transcription polymerase chain reaction detected little PAF receptor messenger ribonucleic acid (mRNA) from FDCs. These data suggest that endogenous production of PAF may be important in the interaction of B lymphocytes with FDCs. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1710-1492 1710-1484 |
| العلاقة: | http://www.aacijournal.com/content/1/2/49Test; https://doaj.org/toc/1710-1484Test; https://doaj.org/toc/1710-1492Test |
| DOI: | 10.1186/1710-1492-1-2-49 |
| الوصول الحر: | https://doaj.org/article/c8bc922649f34b41a2327f00dcf581faTest |
| رقم الانضمام: | edsdoj.8bc922649f34b41a2327f00dcf581fa |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 17101492 17101484 |
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| DOI: | 10.1186/1710-1492-1-2-49 |