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1دورية أكاديمية
المؤلفون: Govindaraju, Gayathri, Kadumuri, Rajashekar Varma, Sethumadhavan, Devadathan Valiyamangalath, Jabeena, C. A., Chavali, Sreenivas, Rajavelu, Arumugam
مصطلحات موضوعية: Genetics, FOS Biological sciences
الوصف: Background Plasmodium falciparum exhibits high translational plasticity during its development in RBCs, yet the regulation at the post-transcriptional level is not well understood. The N6-methyl adenosine (m6A) is an important epigenetic modification primarily present on mRNA that controls the levels of transcripts and efficiency of translation in eukaryotes. Recently, the dynamics of m6A on mRNAs at all three developmental stages of P. falciparum in RBCs have been profiled; however, the proteins that regulate the m6A containing mRNAs in the parasites are unknown. Results Using sequence analysis, we computationally identified that the P. falciparum genome encodes two putative YTH (YT521-B Homology) domain-containing proteins, which could potentially bind to m6A containing mRNA. We developed a modified methylated RNA immunoprecipitation (MeRIP) assay using PfYTH2 and find that it binds selectively to m6A containing transcripts. The PfYTH2 has a conserved aromatic amino acid cage that forms the ...
الإتاحة: https://doi.org/10.6084/m9.figshare.c.5108327Test
https://springernature.figshare.com/collections/N6-Adenosine_methylation_on_mRNA_is_recognized_by_YTH2_domain_protein_of_human_malaria_parasite_Plasmodium_falciparum/5108327Test -
2دورية أكاديمية
المؤلفون: Govindaraju, Gayathri, Kadumuri, Rajashekar Varma, Sethumadhavan, Devadathan Valiyamangalath, Jabeena, C. A., Chavali, Sreenivas, Rajavelu, Arumugam
مصطلحات موضوعية: Genetics, FOS Biological sciences
الوصف: Background Plasmodium falciparum exhibits high translational plasticity during its development in RBCs, yet the regulation at the post-transcriptional level is not well understood. The N6-methyl adenosine (m6A) is an important epigenetic modification primarily present on mRNA that controls the levels of transcripts and efficiency of translation in eukaryotes. Recently, the dynamics of m6A on mRNAs at all three developmental stages of P. falciparum in RBCs have been profiled; however, the proteins that regulate the m6A containing mRNAs in the parasites are unknown. Results Using sequence analysis, we computationally identified that the P. falciparum genome encodes two putative YTH (YT521-B Homology) domain-containing proteins, which could potentially bind to m6A containing mRNA. We developed a modified methylated RNA immunoprecipitation (MeRIP) assay using PfYTH2 and find that it binds selectively to m6A containing transcripts. The PfYTH2 has a conserved aromatic amino acid cage that forms the ...
الإتاحة: https://doi.org/10.6084/m9.figshare.c.5108327.v110.6084/m9.figshare.c.5108327Test
https://springernature.figshare.com/collections/N6-Adenosine_methylation_on_mRNA_is_recognized_by_YTH2_domain_protein_of_human_malaria_parasite_Plasmodium_falciparum/5108327/1Test -
3دورية أكاديمية
المؤلفون: Govindaraju, Gayathri, Kadumuri, Rajashekar Varma, Sethumadhavan, Devadathan Valiyamangalath, Jabeena, C. A., Chavali, Sreenivas, Rajavelu, Arumugam
المصدر: Epigenetics & Chromatin ; volume 13, issue 1 ; ISSN 1756-8935
مصطلحات موضوعية: Genetics, Molecular Biology
الوصف: An amendment to this paper has been published and can be accessed via the original article.
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4دورية أكاديمية
المؤلفون: Govindaraju, Gayathri, Kadumuri, Rajashekar Varma, Sethumadhavan, Devadathan Valiyamangalath, Jabeena, C. A., Chavali, Sreenivas, Rajavelu, Arumugam
المساهمون: Department of Biotechnology , Ministry of Science and Technology
المصدر: Epigenetics & Chromatin ; volume 13, issue 1 ; ISSN 1756-8935
مصطلحات موضوعية: Genetics, Molecular Biology
الوصف: Background Plasmodium falciparum exhibits high translational plasticity during its development in RBCs, yet the regulation at the post-transcriptional level is not well understood. The N 6 -methyl adenosine (m6A) is an important epigenetic modification primarily present on mRNA that controls the levels of transcripts and efficiency of translation in eukaryotes. Recently, the dynamics of m6A on mRNAs at all three developmental stages of P. falciparum in RBCs have been profiled; however, the proteins that regulate the m6A containing mRNAs in the parasites are unknown. Results Using sequence analysis, we computationally identified that the P. falciparum genome encodes two putative YTH (YT521-B Homology) domain-containing proteins, which could potentially bind to m6A containing mRNA. We developed a modified methylated RNA immunoprecipitation (MeRIP) assay using PfYTH2 and find that it binds selectively to m6A containing transcripts. The PfYTH2 has a conserved aromatic amino acid cage that forms the methyl-binding pocket. Through site-directed mutagenesis experiments and molecular dynamics simulations, we show that F98 residue is important for m6A binding on mRNA. Fluorescence depolarization assay confirmed that PfYTH2 binds to methylated RNA oligos with high affinity. Further, MeRIP sequencing data revealed that PfYTH2 has more permissive sequence specificity on target m6A containing mRNA than other known eukaryotic YTH proteins. Taken together, here we identify and characterize PfYTH2 as the major protein that could regulate m6A containing transcripts in P. falciparum . Conclusion Plasmodium spp. lost the canonical m6A-specific demethylases in their genomes, however, the YTH domain-containing proteins seem to be retained. This study presents a possibility that the YTH proteins are involved in post-transcriptional control in P. falciparum , and might orchestrate the translation of mRNA in various developmental stages of P. falciparum . This is perhaps the first characterization of the methyl-reading ...
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5دورية أكاديمية
المؤلفون: Kadumuri, Rajashekar Varma, Vadrevu, Ramakrishna
المصدر: Journal of Computational Biology ; volume 24, issue 10, page 1043-1049 ; ISSN 1557-8666
مصطلحات موضوعية: Computational Theory and Mathematics, Computational Mathematics, Genetics, Molecular Biology, Modeling and Simulation