PRC2 and EHMT1 regulate H3K27me2 and H3K27me3 establishment across the zygote genome
| العنوان: | PRC2 and EHMT1 regulate H3K27me2 and H3K27me3 establishment across the zygote genome |
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| المؤلفون: | Yi Hou, Xiaoyu Liu, Honglin Liu, Ying-Chun Ouyang, Qing-Yuan Sun, Zheng-Hui Zhao, Xue-Shan Ma, Xiang-Hong Ou, Wen-Long Lei, Shaorong Gao, Tie-Gang Meng, Zhen-Bo Wang, Xian-Ju Huang, Qing-Ren Meng, Qian Zhou, Heide Schatten |
| المصدر: | Nature Communications, Vol 11, Iss 1, Pp 1-12 (2020) Nature Communications |
| بيانات النشر: | Nature Portfolio, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Epigenomics, Male, 0301 basic medicine, Zygote, Heterochromatin, Science, General Physics and Astronomy, macromolecular substances, Biology, Methylation, Article, General Biochemistry, Genetics and Molecular Biology, Histones, Mice, 03 medical and health sciences, Oogenesis, 0302 clinical medicine, Histone post-translational modifications, Animals, Micrococcal Nuclease, Enhancer of Zeste Homolog 2 Protein, Epigenetics, Mice, Knockout, Mice, Inbred ICR, Genome, Multidisciplinary, EZH2, Polycomb Repressive Complex 2, Histone-Lysine N-Methyltransferase, General Chemistry, Male pronucleus, Cell biology, 030104 developmental biology, 030220 oncology & carcinogenesis, biology.protein, PRC2, Protein Processing, Post-Translational, Micrococcal nuclease |
| الوصف: | The formation of zygote is the beginning of mammalian life, and dynamic epigenetic modifications are essential for mammalian normal development. H3K27 di-methylation (H3K27me2) and H3K27 tri-methylation (H3K27me3) are marks of facultative heterochromatin which maintains transcriptional repression established during early development in many eukaryotes. However, the mechanism underlying establishment and regulation of epigenetic asymmetry in the zygote remains obscure. Here we show that maternal EZH2 is required for the establishment of H3K27me3 in mouse zygotes. However, combined immunostaining with ULI-NChIP-seq (ultra-low-input micrococcal nuclease-based native ChIP-seq) shows that EZH1 could partially safeguard the role of EZH2 in the formation of H3K27me2. Meanwhile, we identify that EHMT1 is involved in the establishment of H3K27me2, and that H3K27me2 might be an essential prerequisite for the following de novo H3K27me3 modification on the male pronucleus. In this work, we clarify the establishment and regulatory mechanisms of H3K27me2 and H3K27me3 in mouse zygotes. Dynamic arrangement of epigenetic modifications such as repressive H3K27 methylation is essential for zygote development. Here the authors show that establishment of genome-wide H3K27me3 in zygotes requires EZH2, that EZH1 partially compensates for EZH2 loss, and that EHMT1 is involved in H3K27me2 establishment. |
| اللغة: | English |
| تدمد: | 2041-1723 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bfe28d0a6a7dd5b17e4678594191521bTest https://doaj.org/article/880e838cf60f4e349e02ecc7127b2eb6Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....bfe28d0a6a7dd5b17e4678594191521b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20411723 |
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