دورية أكاديمية
Binding of bromocresol green and bromocresol purple to albumin in hemodialysis patients
| العنوان: | Binding of bromocresol green and bromocresol purple to albumin in hemodialysis patients |
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| المؤلفون: | Delanghe, Sigurd, Van Biesen, Wim, Van de Velde, Nadeige, Eloot, Sunny, Pletinck, Anneleen, Schepers, Eva, Glorieux, Griet, Delanghe, Joris, Speeckaert, Marijn |
| المصدر: | CLINICAL CHEMISTRY AND LABORATORY MEDICINE ; ISSN: 1434-6621 |
| سنة النشر: | 2018 |
| المجموعة: | Ghent University Academic Bibliography |
| مصطلحات موضوعية: | Medicine and Health Sciences, albumin, bromocresol green, bromocresol purple, carbamylation, para-cresyl sulfate, uremic toxins, CHRONIC KIDNEY-DISEASE, BOUND UREMIC SOLUTES, P-CRESYL SULFATE, SERUM-ALBUMIN, INDOXYL SULFATE, ALPHA-1-ACID GLYCOPROTEIN, PROTEIN CARBAMYLATION, ENDOGENOUS LIGANDS, RENAL-FAILURE, TOXINS |
| الوصف: | BACKGROUND: Colorimetric albumin assays based on binding to bromocresol purple (BCP) and bromocresol green (BCG) yield different results in chronic kidney disease. Altered dye binding of carbamylated albumin has been suggested as a cause. In the present study, a detailed analysis was carried out in which uremic toxins, acute phase proteins and Kt/V, a parameter describing hemodialysis efficiency, were compared with colorimetrically assayed (BCP and BCG) serum albumin. METHODS: Albumin was assayed using immunonephelometry on a BN II nephelometer and colorimetrically based on, respectively, BCP and BCG on a Modular P analyzer. Uremic toxins were assessed using high-performance liquid chromatography. Acute phase proteins (C-reactive protein and α1-acid glycoprotein) and plasma protein α2-macroglobulin were assayed nephelometrically. In parallel, Kt/V was calculated. RESULTS: Sixty-two serum specimens originating from hemodialysis patients were analyzed. Among the uremic toxins investigated, total para-cresyl sulfate (PCS) showed a significant positive correlation with the BCP/BCG ratio. The serum α1-acid glycoprotein concentration correlated negatively with the BCP/BCG ratio. The BCP/BCG ratio showed also a negative correlation with Kt/V. CONCLUSIONS: In renal insufficiency, the BCP/BCG ratio of serum albumin is affected by multiple factors: next to carbamylation, uremic toxins (total PCS) and α1-acid glycoprotein also play a role. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | https://biblio.ugent.be/publication/8542865Test; http://hdl.handle.net/1854/LU-8542865Test; http://dx.doi.org/10.1515/cclm-2017-0444Test; https://biblio.ugent.be/publication/8542865/file/8542866Test |
| DOI: | 10.1515/cclm-2017-0444 |
| الإتاحة: | https://doi.org/10.1515/cclm-2017-0444Test https://biblio.ugent.be/publication/8542865Test http://hdl.handle.net/1854/LU-8542865Test https://biblio.ugent.be/publication/8542865/file/8542866Test |
| حقوق: | No license (in copyright) ; info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.44EEE30E |
| قاعدة البيانات: | BASE |
| DOI: | 10.1515/cclm-2017-0444 |
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