دورية أكاديمية
Immunoexpression of DNA fragmentation factor 40, DNA fragmentation factor 45, and B-cell lymphoma 2 protein in normal human endometrium and uterine myometrium depends on menstrual cycle phase and menopausal status
| العنوان: | Immunoexpression of DNA fragmentation factor 40, DNA fragmentation factor 45, and B-cell lymphoma 2 protein in normal human endometrium and uterine myometrium depends on menstrual cycle phase and menopausal status |
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| المؤلفون: | Banaś, Tomasz, Pityński, Kazimierz, Okoń, Krzysztof, Mikos, Marcin, Czerw, Aleksandra I., Deptała, Andrzej, Ludwin, Artur |
| سنة النشر: | 2018 |
| المجموعة: | Jagiellonian University Repository |
| مصطلحات موضوعية: | endometrium, Bcl-2, menstrual cycle, DNA fragmentation factors 40, DFF40, DNA fragmentation factor 45, DFF45, B-cell lymphoma 2, uterine myometrium |
| الوصف: | Introduction: DNA fragmentation factors 40 and 45 (DFF40 and DFF45) are final executors of apoptosis, and B-cell lymphoma 2 (Bcl-2) is a well-recognized apoptosis inhibitor. We aimed to evaluate DFF40, DFF45 and Bcl-2 immunoexpression in the normal human endometrium with respect to the glandular and stromal layer and in uterine myometrium. Material and methods: DFF40, DFF45, and Bcl-2 expression was assessed via immunohistochemistry in the endometrium and myometrium collected postmenopausally and premenopausally during the proliferative and secretory phases of the menstrual cycle. Results: Compared to the myometrium and stroma, endometrial glands showed the highest DFF40 and DFF45 expression in pre- and postmenopausal specimens. DFF45, but not DFF40, glandular expression dependent on menstrual cycle phase and DFF40 and DFF45 scoring was significantly lower in postmenopausal specimens. Significantly higher Bcl-2 expression was observed in proliferative glandular endometrium compared to secretory and postmenopausal specimens. No cycle- or menopause-dependent changes were reported for stromal or myometrial DFF40, DFF45 or Bcl-2 expression. DFF40, DFF45 and Bcl-2 expression was independent of age, age at menarche and menopause, BMI, menstrual cycle and menses lengths, parity and gravidity. Conclusions: The study provides important evidence regarding menstrual cycle-dependent changes in the expression of DFF40, DFF45 and Bcl-2 in the normal human endometrium, especially in the glandular layer, and shows that their levels are stable in the normal uterine myometrium. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1734-1922 1896-9151 |
| العلاقة: | Archives of Medical Science, T. 14, nr 6, s. 1254-1262; https://ruj.uj.edu.pl/xmlui/handle/item/141118Test |
| DOI: | 10.5114/aoms.2017.69383 |
| الإتاحة: | https://doi.org/10.5114/aoms.2017.69383Test https://ruj.uj.edu.pl/xmlui/handle/item/141118Test |
| حقوق: | Udzielam licencji. Uznanie autorstwa - Użycie niekomercyjne - Na tych samych warunkach 4.0 Międzynarodowa ; http://creativecommons.org/licenses/by-nc-sa/4.0/legalcode.plTest |
| رقم الانضمام: | edsbas.9CC6C7EB |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 17341922 18969151 |
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| DOI: | 10.5114/aoms.2017.69383 |