المؤلفون: Jung-Hyun Kim, Ssang-Goo Cho, Yong-Jin Kang, Eung-Ryoung Lee

المصدر: Biological and Pharmaceutical Bulletin. 30:32-37

مصطلحات موضوعية: Keratinocytes, Cell type, Programmed cell death, Time Factors, Cell Survival, Ultraviolet Rays, Pharmaceutical Science, Apoptosis, Biology, Antioxidants, Cell Line, Structure-Activity Relationship, chemistry.chemical_compound, Humans, Cell Proliferation, Flavonoids, Pharmacology, chemistry.chemical_classification, Reactive oxygen species, Molecular Structure, Caspase 3, Cell growth, Dose-Response Relationship, Radiation, General Medicine, Eriodictyol, Enzyme Activation, HaCaT, chemistry, Biochemistry, Cell culture, Flavanones, Poly(ADP-ribose) Polymerases, Reactive Oxygen Species, Sunscreening Agents

الوصف: Recently, considerable scientific and therapeutic interest has focused on the structure and functions of the flavonoids. In a previous study, we suggested that hydroxyl (OH) substitutions on specific carbons in the skeleton of the flavonoids might significantly affect their apoptosis-modulating properties. Here, to investigate the effect of various OH substitutions on their diphenylpropane (C6C3C6) skeleton carbons, we selected 10 different flavonoids and assessed their role on UV-induced apoptosis of human keratinocytes, the principal cell type of epidermis. The results showed that 5,7,3',4'-tetrahydroxylflavanone (eriodictyol) and 3,4'-dihydroxy flavone (3,4'-DHF) had a positive effect on cell proliferation of human HaCaT keratinocytes. Treatment with eriodictyol in particular resulted in significant suppression of cell death induced by ultraviolet (UV) light, a major skin-damaging agent. We found that eriodictyol treatment apparently reduced the percentage of apoptotic cells and the cleavage of poly(ADP-ribose) polymerase, concomitant with the repression of caspase-3 activation and reactive oxygen species (ROS) generation. The anti-apoptotic and anti-oxidant effects of eriodictyol were also confirmed in UV-induced cell death of normal human epidermal keratinocyte (NHEK) cells. Taken together, these findings suggest that eriodictyol can be used to protect keratinocytes from UV-induced damage, implying the presence of a complex structure-activity relationship (SAR) in the differential apoptosis-modulating activities of various flavonoids.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a1affab94f1fcc67eb3e917776e1e68eTest
https://doi.org/10.1248/bpb.30.32Test

المؤلفون: Sol Kim, Young Bong Kim, Bong-Woo Kim, Hye Yeon Choi, Sujeong Kim, Ssang-Goo Cho, Hae Na Lee, Jungho Kim, Minjung Song, Seung Woo Kim, Jin-Hoi Kim

المصدر: PLoS ONE
PLoS ONE, Vol 9, Iss 6, p e99421 (2014)

مصطلحات موضوعية: Proteomics, Keratinocytes, Hot Temperature, Antioxidant, Cell Survival, Science, medicine.medical_treatment, Sus scrofa, Apoptosis, Biology, Biochemistry, Antioxidants, Cell Line, Biomaterials, Necrosis, Placenta, Molecular Cell Biology, Placental Extracts, medicine, Animals, Humans, Viability assay, Cell Proliferation, Multidisciplinary, Estradiol, Cell growth, Biology and Life Sciences, Cell Biology, Hydrogen Peroxide, Chromatography, Ion Exchange, Molecular biology, Oxidative Stress, HaCaT, medicine.anatomical_structure, Chromatography, Gel, Medicine, Female, alpha-Fetoproteins, Alpha-fetoprotein, Biomarkers, Research Article, Biotechnology

الوصف: Placenta, as a reservoir of nutrients, has been widely used in medical and cosmetic materials. Here, we focused on the antioxidant properties of placental extract and attempted to isolate and identify the main antioxidant factors. Porcine placental extracts were prepared through homogenization or acid hydrolysis, and their antioxidant activity was investigated in the human keratinocyte HaCaT cell line. Treatment with homogenized placental extract (H-PE) increased the cell viability of H2O2-treated HaCaT cells more than two-fold. H-PE treatment suppressed H2O2-induced apoptotic and necrotic cell death and decreased intracellular ROS levels in H2O2-treated HaCaT cells. The antioxidant factors in H-PE were found to be thermo-unstable and were thus expected to include proteins. The candidate antioxidant proteins were fractionated with cation-exchange, anion-exchange, and size-exclusion chromatography, and the antioxidant properties of the chromatographic fractions were investigated. We obtained specific antioxidant fractions that suppressed ROS generation and ROS-induced DNA strand breaks. From silver staining and MALDI-TOF analyses, alpha-fetoprotein (AFP) precursor was identified as a main marker for the antioxidant effect of H-PE. Purified AFP or ectopically expressed AFP exhibited synergistic antioxidant activity in the presence of estradiol. Taken together, our data suggest that AFP, a serum glycoprotein produced at high levels during fetal development, is a novel marker protein for the antioxidant effect of the placenta that exhibits synergistic antioxidant activity in the presence of estradiol.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a6b53c2b080b7018b4aa6a28f0875778Test
https://doi.org/10.1371/journal.pone.0099421Test