The Anti-apoptotic and Anti-oxidant Effect of Eriodictyol on UV-Induced Apoptosis in Keratinocytes
| العنوان: | The Anti-apoptotic and Anti-oxidant Effect of Eriodictyol on UV-Induced Apoptosis in Keratinocytes |
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| المؤلفون: | Jung-Hyun Kim, Ssang-Goo Cho, Yong-Jin Kang, Eung-Ryoung Lee |
| المصدر: | Biological and Pharmaceutical Bulletin. 30:32-37 |
| بيانات النشر: | Pharmaceutical Society of Japan, 2007. |
| سنة النشر: | 2007 |
| مصطلحات موضوعية: | Keratinocytes, Cell type, Programmed cell death, Time Factors, Cell Survival, Ultraviolet Rays, Pharmaceutical Science, Apoptosis, Biology, Antioxidants, Cell Line, Structure-Activity Relationship, chemistry.chemical_compound, Humans, Cell Proliferation, Flavonoids, Pharmacology, chemistry.chemical_classification, Reactive oxygen species, Molecular Structure, Caspase 3, Cell growth, Dose-Response Relationship, Radiation, General Medicine, Eriodictyol, Enzyme Activation, HaCaT, chemistry, Biochemistry, Cell culture, Flavanones, Poly(ADP-ribose) Polymerases, Reactive Oxygen Species, Sunscreening Agents |
| الوصف: | Recently, considerable scientific and therapeutic interest has focused on the structure and functions of the flavonoids. In a previous study, we suggested that hydroxyl (OH) substitutions on specific carbons in the skeleton of the flavonoids might significantly affect their apoptosis-modulating properties. Here, to investigate the effect of various OH substitutions on their diphenylpropane (C6C3C6) skeleton carbons, we selected 10 different flavonoids and assessed their role on UV-induced apoptosis of human keratinocytes, the principal cell type of epidermis. The results showed that 5,7,3',4'-tetrahydroxylflavanone (eriodictyol) and 3,4'-dihydroxy flavone (3,4'-DHF) had a positive effect on cell proliferation of human HaCaT keratinocytes. Treatment with eriodictyol in particular resulted in significant suppression of cell death induced by ultraviolet (UV) light, a major skin-damaging agent. We found that eriodictyol treatment apparently reduced the percentage of apoptotic cells and the cleavage of poly(ADP-ribose) polymerase, concomitant with the repression of caspase-3 activation and reactive oxygen species (ROS) generation. The anti-apoptotic and anti-oxidant effects of eriodictyol were also confirmed in UV-induced cell death of normal human epidermal keratinocyte (NHEK) cells. Taken together, these findings suggest that eriodictyol can be used to protect keratinocytes from UV-induced damage, implying the presence of a complex structure-activity relationship (SAR) in the differential apoptosis-modulating activities of various flavonoids. |
| تدمد: | 1347-5215 0918-6158 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a1affab94f1fcc67eb3e917776e1e68eTest https://doi.org/10.1248/bpb.30.32Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....a1affab94f1fcc67eb3e917776e1e68e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13475215 09186158 |
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