Alpha-Fetoprotein, Identified as a Novel Marker for the Antioxidant Effect of Placental Extract, Exhibits Synergistic Antioxidant Activity in the Presence of Estradiol
| العنوان: | Alpha-Fetoprotein, Identified as a Novel Marker for the Antioxidant Effect of Placental Extract, Exhibits Synergistic Antioxidant Activity in the Presence of Estradiol |
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| المؤلفون: | Sol Kim, Young Bong Kim, Bong-Woo Kim, Hye Yeon Choi, Sujeong Kim, Ssang-Goo Cho, Hae Na Lee, Jungho Kim, Minjung Song, Seung Woo Kim, Jin-Hoi Kim |
| المصدر: | PLoS ONE PLoS ONE, Vol 9, Iss 6, p e99421 (2014) |
| بيانات النشر: | Public Library of Science (PLoS), 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Proteomics, Keratinocytes, Hot Temperature, Antioxidant, Cell Survival, Science, medicine.medical_treatment, Sus scrofa, Apoptosis, Biology, Biochemistry, Antioxidants, Cell Line, Biomaterials, Necrosis, Placenta, Molecular Cell Biology, Placental Extracts, medicine, Animals, Humans, Viability assay, Cell Proliferation, Multidisciplinary, Estradiol, Cell growth, Biology and Life Sciences, Cell Biology, Hydrogen Peroxide, Chromatography, Ion Exchange, Molecular biology, Oxidative Stress, HaCaT, medicine.anatomical_structure, Chromatography, Gel, Medicine, Female, alpha-Fetoproteins, Alpha-fetoprotein, Biomarkers, Research Article, Biotechnology |
| الوصف: | Placenta, as a reservoir of nutrients, has been widely used in medical and cosmetic materials. Here, we focused on the antioxidant properties of placental extract and attempted to isolate and identify the main antioxidant factors. Porcine placental extracts were prepared through homogenization or acid hydrolysis, and their antioxidant activity was investigated in the human keratinocyte HaCaT cell line. Treatment with homogenized placental extract (H-PE) increased the cell viability of H2O2-treated HaCaT cells more than two-fold. H-PE treatment suppressed H2O2-induced apoptotic and necrotic cell death and decreased intracellular ROS levels in H2O2-treated HaCaT cells. The antioxidant factors in H-PE were found to be thermo-unstable and were thus expected to include proteins. The candidate antioxidant proteins were fractionated with cation-exchange, anion-exchange, and size-exclusion chromatography, and the antioxidant properties of the chromatographic fractions were investigated. We obtained specific antioxidant fractions that suppressed ROS generation and ROS-induced DNA strand breaks. From silver staining and MALDI-TOF analyses, alpha-fetoprotein (AFP) precursor was identified as a main marker for the antioxidant effect of H-PE. Purified AFP or ectopically expressed AFP exhibited synergistic antioxidant activity in the presence of estradiol. Taken together, our data suggest that AFP, a serum glycoprotein produced at high levels during fetal development, is a novel marker protein for the antioxidant effect of the placenta that exhibits synergistic antioxidant activity in the presence of estradiol. |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a6b53c2b080b7018b4aa6a28f0875778Test https://doi.org/10.1371/journal.pone.0099421Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....a6b53c2b080b7018b4aa6a28f0875778 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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