IL-33/ST2 Axis Plays a Protective Effect in Streptococcus pyogenes Infection through Strengthening of the Innate Immunity
| العنوان: | IL-33/ST2 Axis Plays a Protective Effect in Streptococcus pyogenes Infection through Strengthening of the Innate Immunity |
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| المؤلفون: | Yu-Hsuan Tsai, Wei-Yu Chen, Chih-Feng Kuo, Ya-Chu Chang, Nina Tsao, Chih Peng Chang, Hai-Han Yu |
| المصدر: | International Journal of Molecular Sciences, Vol 22, Iss 10566, p 10566 (2021) International Journal of Molecular Sciences Volume 22 Issue 19 |
| بيانات النشر: | MDPI AG, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Neutrophils, Streptococcus pyogenes, the innate immunity, QH301-705.5, Biology, medicine.disease_cause, Article, Catalysis, Microbiology, Proinflammatory cytokine, Inorganic Chemistry, Cell Movement, Streptococcal Infections, medicine, Animals, Humans, Viability assay, Physical and Theoretical Chemistry, Biology (General), Molecular Biology, QD1-999, Spectroscopy, Gene knockout, Inflammation, Mice, Knockout, Innate immune system, Streptococcus, Effector, Organic Chemistry, General Medicine, Interleukin-33, medicine.disease, Group A Streptococcus (GAS), Interleukin-1 Receptor-Like 1 Protein, suppression of tumorigenicity 2 (ST2), Immunity, Innate, Computer Science Applications, Mice, Inbred C57BL, Interleukin 33, Disease Models, Animal, interleukin-33 (IL-33), Chemistry, Cytokines, Cytokine storm, Signal Transduction |
| الوصف: | Group A Streptococcus (GAS) causes invasive human diseases with the cytokine storm. Interleukin-33 (IL-33)/suppression of tumorigenicity 2 (ST2) axis is known to drive TH2 response, while its effect on GAS infection is unclear. We used an air pouch model to examine the effect of the IL-33/ST2 axis on GAS-induced necrotizing fasciitis. GAS infection induced IL-33 expression in wild-type (WT) C57BL/6 mice, whereas the IL-33- and ST2-knockout mice had higher mortality rates, more severe skin lesions and higher bacterial loads in the air pouches than those of WT mice after infection. Surveys of infiltrating cells in the air pouch of GAS-infected mice at the early stage found that the number and cell viability of infiltrating cells in both gene knockout mice were lower than those of WT mice. The predominant effector cells in GAS-infected air pouches were neutrophils. Absence of the IL-33/ST2 axis enhanced the expression of inflammatory cytokines, but not TH1 or TH2 cytokines, in the air pouch after infection. Using in vitro assays, we found that the IL-33/ST2 axis not only enhanced neutrophil migration but also strengthened the bactericidal activity of both sera and neutrophils. These results suggest that the IL-33/ST2 axis provided the protective effect on GAS infection through enhancing the innate immunity. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1661-6596 1422-0067 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::66bea046e4b2a93f401776f16f3c3875Test https://www.mdpi.com/1422-0067/22/19/10566Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....66bea046e4b2a93f401776f16f3c3875 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16616596 14220067 |
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