دورية
Multicenter Comparative Evaluation of Six Commercial Systems and the National Committee for Clinical Laboratory Standards M27-A Broth Microdilution Method for Fluconazole Susceptibility Testing of CandidaSpecies
| العنوان: | Multicenter Comparative Evaluation of Six Commercial Systems and the National Committee for Clinical Laboratory Standards M27-A Broth Microdilution Method for Fluconazole Susceptibility Testing of CandidaSpecies |
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| المؤلفون: | Morace, G., Amato, G., Bistoni, F., Fadda, G., Marone, P., Montagna, M. T., Oliveri, S., Polonelli, L., Rigoli, R., Mancuso, I., La Face, S., Masucci, L., Romano, L., Napoli, C., Tato`, D., Buscema, M. G., Belli, C. M. C., Piccirillo, M. M., Conti, S., Covan, S., Fanti, F., Cavanna, C., D'Alo`, F., Pitzurra, L. |
| المصدر: | Journal of Clinical Microbiology; August 2002, Vol. 40 Issue: 8 p2953-2958, 6p |
| مستخلص: | ABSTRACTFluconazole susceptibility among 800 clinical Candidaisolates (60% C. albicans) and two control strains (C. kruseiATCC 6258 and C. parapsilosisATCC 22019) was tested with the NCCLS M27-A method (gold standard) and six commercial products (Candifast, disk, Etest, Fungitest, Integral System Yeasts, and Sensititre YeastOne). Results were classified as susceptible, susceptible-dose dependent, or resistant using M27-A breakpoints or, for Fungitest, Integral System Yeasts, and Candifast, as susceptible, intermediate, or resistant, according to the manufacturers' instructions. Concordance with NCCLS M27-A results was analyzed with the ?2test. Intra- and interlaboratory reproducibility was also evaluated. NCCLS M27-A (90.1%), Etest (93.1%), Sensititre YeastOne (93.1%), disk (96.7%), Fungitest (92.6%), Integral System Yeasts (40.6%), and Candifast (6.0%) classified the indicated percentages of C. albicansisolates as susceptible. Among non-C. albicansstrains, the percentages of susceptible isolates were as follows: NCCLS M27-A, 74.0%; Etest, 83.8%; Sensititre YeastOne, 64.1%; disk, 60.6%; Fungitest, 76.6%; Integral System Yeasts, 28.3%; and Candifast, 27.4%. All methods except Candifast and Integral System Yeasts showed good agreement with NCCLS M27-A results for both C albicansand non-C. albicansisolates. Intralaboratory reproducibility was excellent for NCCLS M27-A, Etest, Sensititre YeastOne, disk, and Fungitest (88 to 91%). Similar results emerged from the interlaboratory reproducibility evaluation. Our findings indicate that some commercial methods can be useful for fluconazole susceptibility testing of clinical Candidaisolates. Those characterized by a lack of medium standardization and/or objective interpretative criteria should be avoided. Particular caution is necessary when testing is being done for clinical and epidemiological purposes. |
| قاعدة البيانات: | Supplemental Index |
Full Text Finder | تدمد: | 00951137 1098660X |
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| DOI: | 10.1128/JCM.40.8.2953-2958.2002 |