Mechanical Antiallodynic Effect of Intrathecal Nefopam in a Rat Neuropathic Pain Model
| العنوان: | Mechanical Antiallodynic Effect of Intrathecal Nefopam in a Rat Neuropathic Pain Model |
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| المؤلفون: | Gyeong-Jo Byeon, Sungtae Koo, Sang Wook Shin, Kyung-Hoon Kim, Hyun-Chul Kim, Seung-Hoon Baek |
| المصدر: | Journal of Korean Medical Science |
| بيانات النشر: | The Korean Academy of Medical Sciences, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Mechanical Allodynia, Male, Pharmacology, CREB, Spinal Nerve Ligation, Rats, Sprague-Dawley, chemistry.chemical_compound, Nefopam, medicine, Animals, Cyclic adenosine monophosphate, Injections, Spinal, Neuropathic Pain, Pain Measurement, biology, Glial fibrillary acidic protein, Dose-Response Relationship, Drug, business.industry, Anesthesiology & Pain, Pain Perception, General Medicine, Analgesics, Non-Narcotic, medicine.disease, Spinal cord, Rats, medicine.anatomical_structure, Treatment Outcome, chemistry, Hyperalgesia, Anesthesia, Neuropathic pain, biology.protein, Neuralgia, Original Article, medicine.symptom, business, medicine.drug |
| الوصف: | Nefopam has a pharmacologic profile distinct from that of opioids or other anti-inflammatory drugs. Several recent studies demonstrate that nefopam has a mechanism of action similar to those of anti-depressants and anticonvulsants for treating neuropathic pain. The present study investigates the mechanical antiallodynic effect of nefopam using immunohistochemical study and western blot analysis in a rat neuropathic pain model. Twenty-eight male Sprague-Dawley rats were subjected to left fifth lumbar (L5) spinal nerve ligation and intrathecal catheter implantation, procedures which were not performed on the 7 male Sprague-Dawley rats in the sham surgery group (group S). Nefopam, either 10 or 100 µg/kg (group N10 or N100, respectively), and normal saline (group C) were intrathecally administered into the catheter every day for 14 days. The mechanical allodynic threshold of intrathecal nefopam was measured using a dynamic plantar aesthesiometer. Immunohistochemistry targeting cluster of differentiation molecule 11b (CD11b) and glial fibrillary acidic protein (GFAP) was performed on the harvested spinal cord at the level of L5. Extracellular signal-regulated kinase 1/2 (ERK 1/2) and cyclic adenosine monophosphate response element binding protein (CREB) were measured using western blot analysis. The N10 and N100 groups showed improved mechanical allodynic threshold, reduced CD11b and GFAP expression, and attenuated ERK 1/2 and CREB in the affected L5 spinal cord. In conclusion, intrathecal nefopam reduced mechanical allodynia in a rat neuropathic pain model. Its mechanical antiallodynic effect is associated with inhibition of glial activation and suppression of the transcription factors' mitogen-activated protein kinases in the spinal cord. Graphical Abstract |
| اللغة: | English |
| تدمد: | 1598-6357 1011-8934 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::26273c6b1b946742e6d75717fece8093Test http://europepmc.org/articles/PMC4520952Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....26273c6b1b946742e6d75717fece8093 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15986357 10118934 |
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